Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney

The present study investigated the effect of left ventricular hypertrophy (LVH) on the responsiveness of (II-adrenergic receptor subtypes to adrenergic stimuli in the rat. The role of hydrogen sulphide (H2S) and nitric oxide (NO) systems and their interaction in the progression of LVH was studied...

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Main Author: Ahmad, Ashfaq
Format: Thesis
Language:English
Published: 2016
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Online Access:http://eprints.usm.my/57701/1/00001808094%20Ashfaq%20Ahmad24.pdf
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spelling my-usm-ep.577012023-03-31T18:33:46Z Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney 2016-06 Ahmad, Ashfaq RS1-441 Pharmacy and materia medica The present study investigated the effect of left ventricular hypertrophy (LVH) on the responsiveness of (II-adrenergic receptor subtypes to adrenergic stimuli in the rat. The role of hydrogen sulphide (H2S) and nitric oxide (NO) systems and their interaction in the progression of LVH was studied by examining the effect of altered expression of cystathione y lyase (CSE mRNA) and endothelial nitric oxide synthase (eNOS mRN A) in the heart during LVH. Cardiovascular parameters such as cardiac geometry, oxidative stress, arterial stiffness and vascular responsiveness to vasoactive stimuli were studied. In addition, this study examined renal excretory functions, haemodynamics and histopathological changes after exogenous administration of NaHS, an H2S donor, L-arginine, an NO donor and a combination of NaHS and L-arginine. Wistar-Kyoto (WKY) rats were divided into two major groups of Control and LVH. These groups were then subdivided into 8 groups based on treatment. NaHS (56JlM/kg I.P. for 5 weeks) or L-arginine (1.25g1L for 5 weeks in drinking water) treated control or LVH groups. LVH was induced using isoprenaline (Smg/kg, S.c. every 72 hours for 2 weeks) and caffeine (62mglL in drinking water for 2 weeks). The uj-adrenergic receptors subtypes was studied by examining the responsiveness to noradrenaline (NA), phenylephrine (PE) and methoxamine (ME) in the presence of a background intrarenal infusion of selective uj-adrenergic receptors blockers (5-methylurapidil (5-MeU), chloroethy1clonidine (CEC) and BMY 7378). Real-time quantitative peR data (normalized to 2016-06 Thesis http://eprints.usm.my/57701/ http://eprints.usm.my/57701/1/00001808094%20Ashfaq%20Ahmad24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia. Pusat Pengajian Sains Farmasi
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic RS1-441 Pharmacy and materia medica
spellingShingle RS1-441 Pharmacy and materia medica
Ahmad, Ashfaq
Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney
description The present study investigated the effect of left ventricular hypertrophy (LVH) on the responsiveness of (II-adrenergic receptor subtypes to adrenergic stimuli in the rat. The role of hydrogen sulphide (H2S) and nitric oxide (NO) systems and their interaction in the progression of LVH was studied by examining the effect of altered expression of cystathione y lyase (CSE mRNA) and endothelial nitric oxide synthase (eNOS mRN A) in the heart during LVH. Cardiovascular parameters such as cardiac geometry, oxidative stress, arterial stiffness and vascular responsiveness to vasoactive stimuli were studied. In addition, this study examined renal excretory functions, haemodynamics and histopathological changes after exogenous administration of NaHS, an H2S donor, L-arginine, an NO donor and a combination of NaHS and L-arginine. Wistar-Kyoto (WKY) rats were divided into two major groups of Control and LVH. These groups were then subdivided into 8 groups based on treatment. NaHS (56JlM/kg I.P. for 5 weeks) or L-arginine (1.25g1L for 5 weeks in drinking water) treated control or LVH groups. LVH was induced using isoprenaline (Smg/kg, S.c. every 72 hours for 2 weeks) and caffeine (62mglL in drinking water for 2 weeks). The uj-adrenergic receptors subtypes was studied by examining the responsiveness to noradrenaline (NA), phenylephrine (PE) and methoxamine (ME) in the presence of a background intrarenal infusion of selective uj-adrenergic receptors blockers (5-methylurapidil (5-MeU), chloroethy1clonidine (CEC) and BMY 7378). Real-time quantitative peR data (normalized to
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Ahmad, Ashfaq
author_facet Ahmad, Ashfaq
author_sort Ahmad, Ashfaq
title Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney
title_short Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney
title_full Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney
title_fullStr Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney
title_full_unstemmed Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney
title_sort interaction between hydrogen sulphide (h2s) and nitric oxide (no) in left ventricular hypertrophy and their effect on responsiveness of alpha i-adrenergic receptors subtypes in the rat kidney
granting_institution Universiti Sains Malaysia.
granting_department Pusat Pengajian Sains Farmasi
publishDate 2016
url http://eprints.usm.my/57701/1/00001808094%20Ashfaq%20Ahmad24.pdf
_version_ 1776101207196041216