Designing a multiepitope vaccine against toxoplasma gondii

Toxoplasmosis is a significant, life-threatening disease with medical, veterinary, and economic importance caused by Toxoplasma gondii. T. gondii infects about 25-30% of human population globally. There have been noteworthy efforts to control and limit the disease incidence. At present, the only app...

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Main Author:	Folasade, Hammed-Akanmu Mutiat
Format:	Thesis
Language:	English
Published:	2021
Subjects:	QL360-599.82 Invertebrates
Online Access:	http://eprints.usm.my/58212/1/Hammed-Akanmu-24%20pages.pdf
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id	my-usm-ep.58212
record_format	uketd_dc
spelling	my-usm-ep.582122023-04-27T07:01:29Z Designing a multiepitope vaccine against toxoplasma gondii 2021-10 Folasade, Hammed-Akanmu Mutiat QL360-599.82 Invertebrates Toxoplasmosis is a significant, life-threatening disease with medical, veterinary, and economic importance caused by Toxoplasma gondii. T. gondii infects about 25-30% of human population globally. There have been noteworthy efforts to control and limit the disease incidence. At present, the only approved vaccine for use in veterinary is attenuated tachyzoites of strain S48 that control congenital infection of ewe known as Toxovax, which has significantly reduced the rate of abortion in sheep. However, the vaccine is expensive and has the probability of changing into a pathogenic form thus, it is inappropriate for human use. At the moment, there is no effective vaccine for preventing the formation of chronic tissue cysts in an infected host. Therefore, this research aims to design a multiepitope vaccine against T. gondii using in silico method to predict and analyze B-cell and T-cell epitopes of ROP2, MIC3, and GRA7. The result showed that the selected epitopes were antigenic, non-allergen, non-toxic, and non-human homology which makes them appropriate for the construction of vaccines. The secondary and tertiary structure, as well as the physiochemical properties of the vaccine construct, were determined and validation experiments such as molecular docking and immune simulation were conducted. This validation process predicted that the candidate vaccine is stable and soluble in the biological environment. In conclusion, in silico method can be used to design a good vaccine for T. gondii. 2021-10 Thesis http://eprints.usm.my/58212/ http://eprints.usm.my/58212/1/Hammed-Akanmu-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QL360-599.82 Invertebrates
spellingShingle	QL360-599.82 Invertebrates Folasade, Hammed-Akanmu Mutiat Designing a multiepitope vaccine against toxoplasma gondii
description	Toxoplasmosis is a significant, life-threatening disease with medical, veterinary, and economic importance caused by Toxoplasma gondii. T. gondii infects about 25-30% of human population globally. There have been noteworthy efforts to control and limit the disease incidence. At present, the only approved vaccine for use in veterinary is attenuated tachyzoites of strain S48 that control congenital infection of ewe known as Toxovax, which has significantly reduced the rate of abortion in sheep. However, the vaccine is expensive and has the probability of changing into a pathogenic form thus, it is inappropriate for human use. At the moment, there is no effective vaccine for preventing the formation of chronic tissue cysts in an infected host. Therefore, this research aims to design a multiepitope vaccine against T. gondii using in silico method to predict and analyze B-cell and T-cell epitopes of ROP2, MIC3, and GRA7. The result showed that the selected epitopes were antigenic, non-allergen, non-toxic, and non-human homology which makes them appropriate for the construction of vaccines. The secondary and tertiary structure, as well as the physiochemical properties of the vaccine construct, were determined and validation experiments such as molecular docking and immune simulation were conducted. This validation process predicted that the candidate vaccine is stable and soluble in the biological environment. In conclusion, in silico method can be used to design a good vaccine for T. gondii.
format	Thesis
qualification_level	Master's degree
author	Folasade, Hammed-Akanmu Mutiat
author_facet	Folasade, Hammed-Akanmu Mutiat
author_sort	Folasade, Hammed-Akanmu Mutiat
title	Designing a multiepitope vaccine against toxoplasma gondii
title_short	Designing a multiepitope vaccine against toxoplasma gondii
title_full	Designing a multiepitope vaccine against toxoplasma gondii
title_fullStr	Designing a multiepitope vaccine against toxoplasma gondii
title_full_unstemmed	Designing a multiepitope vaccine against toxoplasma gondii
title_sort	designing a multiepitope vaccine against toxoplasma gondii
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kesihatan
publishDate	2021
url	http://eprints.usm.my/58212/1/Hammed-Akanmu-24%20pages.pdf
_version_	1776101212944334848

Designing a multiepitope vaccine against toxoplasma gondii

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