Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma

The growing interest of anthocyanins in roselle has brought upon the importance of the compound’s analysis to investigate its pharmacological properties. Sensitive and specific analytical methods are required to accurately analyse the anthocyanin present in the sample. In this study, rapid resolutio...

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Main Author:	Sauji, Nadiratul Asyikin
Format:	Thesis
Language:	English
Published:	2021
Subjects:	QD71-142 Analytical chemistry
Online Access:	http://eprints.usm.my/58282/1/Nadiratul-24%20pages.pdf
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spelling	my-usm-ep.582822023-05-15T01:10:45Z Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma 2021-08 Sauji, Nadiratul Asyikin QD71-142 Analytical chemistry The growing interest of anthocyanins in roselle has brought upon the importance of the compound’s analysis to investigate its pharmacological properties. Sensitive and specific analytical methods are required to accurately analyse the anthocyanin present in the sample. In this study, rapid resolution liquid chromatography (RRLC) was used to analyse cyanidin-3-O-glucoside chloride, one of the anthocyanins found in roselle. Spectrophotometric analysis and optimization of chromatographic conditions were performed to develop a suitable and precise RRLC method for the determination of the selected anthocyanin. Both liquid-liquid extraction (LLE) and solid-phase extraction (SPE) method were performed to evaluate the best extraction method of anthocyanin in the rat plasma sample. The optimised chromatographic method demonstrated that the mobile phase composition of 0.1% trifluoroacetic acid aqueous solution and acetonitrile in the ratio of 81:19, respectively with a flow rate of 0.5 mL/min, at 30°C column temperature and detection wavelength of 525 nm were suitable for RRLC analysis of cyanidin-3-O-glucoside chloride. SPE was chosen as the final extraction method in the study as it produced better peaks in the chromatogram compared to LLE. In conclusion, the developed RRLC method in this study can be used to determine anthocyanins in the future pharmacokinetic study of roselle. 2021-08 Thesis http://eprints.usm.my/58282/ http://eprints.usm.my/58282/1/Nadiratul-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QD71-142 Analytical chemistry
spellingShingle	QD71-142 Analytical chemistry Sauji, Nadiratul Asyikin Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma
description	The growing interest of anthocyanins in roselle has brought upon the importance of the compound’s analysis to investigate its pharmacological properties. Sensitive and specific analytical methods are required to accurately analyse the anthocyanin present in the sample. In this study, rapid resolution liquid chromatography (RRLC) was used to analyse cyanidin-3-O-glucoside chloride, one of the anthocyanins found in roselle. Spectrophotometric analysis and optimization of chromatographic conditions were performed to develop a suitable and precise RRLC method for the determination of the selected anthocyanin. Both liquid-liquid extraction (LLE) and solid-phase extraction (SPE) method were performed to evaluate the best extraction method of anthocyanin in the rat plasma sample. The optimised chromatographic method demonstrated that the mobile phase composition of 0.1% trifluoroacetic acid aqueous solution and acetonitrile in the ratio of 81:19, respectively with a flow rate of 0.5 mL/min, at 30°C column temperature and detection wavelength of 525 nm were suitable for RRLC analysis of cyanidin-3-O-glucoside chloride. SPE was chosen as the final extraction method in the study as it produced better peaks in the chromatogram compared to LLE. In conclusion, the developed RRLC method in this study can be used to determine anthocyanins in the future pharmacokinetic study of roselle.
format	Thesis
qualification_level	Master's degree
author	Sauji, Nadiratul Asyikin
author_facet	Sauji, Nadiratul Asyikin
author_sort	Sauji, Nadiratul Asyikin
title	Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma
title_short	Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma
title_full	Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma
title_fullStr	Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma
title_full_unstemmed	Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma
title_sort	development and optimization of rapid resolution liquid chromatography (rrlc) method following solid-phase extraction for determination of anthocyanin in rat plasma
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kesihatan
publishDate	2021
url	http://eprints.usm.my/58282/1/Nadiratul-24%20pages.pdf
_version_	1776101215338233856

Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma

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