Elucidation of the vasorelaxation mechanisms induced by syzygium polyanthum leaves aqueous extract
Previous studies have demonstrated that aqueous extract of Syzygium polyanthum leaves (AESP) has vasorelaxation activity. However, there is still very little information on its mechanism of action on direct vasorelaxation activity. Therefore, the present study was designed to determine endotheliu...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2021
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/58289/1/Nuriana%20Munirah-24%20pages.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
my-usm-ep.58289 |
|---|---|
| record_format |
uketd_dc |
| spelling |
my-usm-ep.582892023-05-15T01:24:16Z Elucidation of the vasorelaxation mechanisms induced by syzygium polyanthum leaves aqueous extract 2021-08 Hairul, Nuriana Munirah QK495 Angiosperms Previous studies have demonstrated that aqueous extract of Syzygium polyanthum leaves (AESP) has vasorelaxation activity. However, there is still very little information on its mechanism of action on direct vasorelaxation activity. Therefore, the present study was designed to determine endothelium-dependant relaxation of the AESP and investigated the mechanism of vasorelaxation. The in vitro method was utilised, mounting the aortic ring into myograph, precontracted with phenylephrine (1 μM) and studied based on the agonist-antagonist inhibition protocols. The endothelium-dependant was studied on endothelium intact and denuded thoracic arterial ring isolated from Wistar-Kyoto (WKY) rats. The vasorelaxation mechanisms of AESP were evaluated on the endothelial-intact aortic rings. This study shown that the AESP relaxation was endothelium-dependant. The vasorelaxant effect of AESP was attenuated by L-NAME (endothelial nitric oxide synthase/eNOS inhibitor, 100 μM) and ODQ (soluble guanylate cyclase/sGC inhibitor, 10 μM). However, methylene blue (cyclic guanosine monophosphate/cGMP lowering agent, 10 μM) and indomethacin (non-selective COX inhibitor, 10 μM) did not produce significant inhibition on the AESP relaxation. Taken together, the findings indicated that vasorelaxation of AESP was endothelium-dependent, through NO/eNOS and sGC pathways. 2021-08 Thesis http://eprints.usm.my/58289/ http://eprints.usm.my/58289/1/Nuriana%20Munirah-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan |
| institution |
Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
QK495 Angiosperms |
| spellingShingle |
QK495 Angiosperms Hairul, Nuriana Munirah Elucidation of the vasorelaxation mechanisms induced by syzygium polyanthum leaves aqueous extract |
| description |
Previous studies have demonstrated that aqueous extract of Syzygium polyanthum
leaves (AESP) has vasorelaxation activity. However, there is still very little
information on its mechanism of action on direct vasorelaxation activity. Therefore,
the present study was designed to determine endothelium-dependant relaxation of the
AESP and investigated the mechanism of vasorelaxation. The in vitro method was
utilised, mounting the aortic ring into myograph, precontracted with phenylephrine (1
μM) and studied based on the agonist-antagonist inhibition protocols. The
endothelium-dependant was studied on endothelium intact and denuded thoracic
arterial ring isolated from Wistar-Kyoto (WKY) rats. The vasorelaxation mechanisms
of AESP were evaluated on the endothelial-intact aortic rings. This study shown that
the AESP relaxation was endothelium-dependant. The vasorelaxant effect of AESP
was attenuated by L-NAME (endothelial nitric oxide synthase/eNOS inhibitor, 100
μM) and ODQ (soluble guanylate cyclase/sGC inhibitor, 10 μM). However, methylene
blue (cyclic guanosine monophosphate/cGMP lowering agent, 10 μM) and
indomethacin (non-selective COX inhibitor, 10 μM) did not produce significant
inhibition on the AESP relaxation. Taken together, the findings indicated that
vasorelaxation of AESP was endothelium-dependent, through NO/eNOS and sGC
pathways. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Hairul, Nuriana Munirah |
| author_facet |
Hairul, Nuriana Munirah |
| author_sort |
Hairul, Nuriana Munirah |
| title |
Elucidation of the vasorelaxation
mechanisms induced by
syzygium polyanthum leaves aqueous extract |
| title_short |
Elucidation of the vasorelaxation
mechanisms induced by
syzygium polyanthum leaves aqueous extract |
| title_full |
Elucidation of the vasorelaxation
mechanisms induced by
syzygium polyanthum leaves aqueous extract |
| title_fullStr |
Elucidation of the vasorelaxation
mechanisms induced by
syzygium polyanthum leaves aqueous extract |
| title_full_unstemmed |
Elucidation of the vasorelaxation
mechanisms induced by
syzygium polyanthum leaves aqueous extract |
| title_sort |
elucidation of the vasorelaxation
mechanisms induced by
syzygium polyanthum leaves aqueous extract |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Pusat Pengajian Sains Kesihatan |
| publishDate |
2021 |
| url |
http://eprints.usm.my/58289/1/Nuriana%20Munirah-24%20pages.pdf |
| _version_ |
1776101215814287360 |