The effect of CRX-527 on pro-inflammatory cytokine production against mycobacterium bovis BCG clone expressing the c-terminus of merozoite surface protein-1 of plasmodium falciparum

Malaria infection is a leading cause of mortality and morbidity in African and Southeast Asian countries. TLR-4 has been shown to be important in malaria immunity and can be possibly use as an adjuvant to enhance the long-lasting immune response against BCG-MSP1C. This research was conducted to dete...

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主要作者:	Yusoff, Wan Raihan Wan
格式:	Thesis
語言:	English
出版:	2021
主題:	QR75-99.5 Bacteria
在線閱讀:	http://eprints.usm.my/58306/1/Wan%20Raihan-24%20pages.pdf
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id	my-usm-ep.58306
record_format	uketd_dc
spelling	my-usm-ep.583062023-05-15T01:38:35Z The effect of CRX-527 on pro-inflammatory cytokine production against mycobacterium bovis BCG clone expressing the c-terminus of merozoite surface protein-1 of plasmodium falciparum 2021-10 Yusoff, Wan Raihan Wan QR75-99.5 Bacteria Malaria infection is a leading cause of mortality and morbidity in African and Southeast Asian countries. TLR-4 has been shown to be important in malaria immunity and can be possibly use as an adjuvant to enhance the long-lasting immune response against BCG-MSP1C. This research was conducted to determine the effects of toll-like receptor 4 (TLR-4) agonist, CRX527, on cellular and humoral immune response against Mycobacterium bovis bacille Calmette-Guérin (BCG) expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum (BCG-MSP1C). In this study six groups of mice (n=6 per group) were injected with 200 μl intraperitoneal phosphate buffered saline T80 (PBS-T80), 200 μl of PBS-T80 containing 2 x 106 cfu/ml of BCG or rBCG in the presence or absence of CRX527. Blood was collected from the tail veins of the mice before the start of the immunisation and 4 weeks after each immunisation. Enzyme linked immunosorbent assay (ELISA) was carried out to measure the production of TNF-α and IL-12 in the sera of the immunized mice. The findings showed that the sera of rBCG without CRX immunised group yielding the most increase in IL-12 followed by the mice injected with rBCG with CRX. In contrast, the entire mice immunised groups showed decrease production for TNF-α after the immunisation. Therefore, this combination of rBCG with TLR4 agonist can be used to create a vaccine that capable of inducing a strong and long-lasting immunity to allow the immune system to be prepared for the emergence of multidrug-resistant strains of malaria diseases. 2021-10 Thesis http://eprints.usm.my/58306/ http://eprints.usm.my/58306/1/Wan%20Raihan-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QR75-99.5 Bacteria
spellingShingle	QR75-99.5 Bacteria Yusoff, Wan Raihan Wan The effect of CRX-527 on pro-inflammatory cytokine production against mycobacterium bovis BCG clone expressing the c-terminus of merozoite surface protein-1 of plasmodium falciparum
description	Malaria infection is a leading cause of mortality and morbidity in African and Southeast Asian countries. TLR-4 has been shown to be important in malaria immunity and can be possibly use as an adjuvant to enhance the long-lasting immune response against BCG-MSP1C. This research was conducted to determine the effects of toll-like receptor 4 (TLR-4) agonist, CRX527, on cellular and humoral immune response against Mycobacterium bovis bacille Calmette-Guérin (BCG) expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum (BCG-MSP1C). In this study six groups of mice (n=6 per group) were injected with 200 μl intraperitoneal phosphate buffered saline T80 (PBS-T80), 200 μl of PBS-T80 containing 2 x 106 cfu/ml of BCG or rBCG in the presence or absence of CRX527. Blood was collected from the tail veins of the mice before the start of the immunisation and 4 weeks after each immunisation. Enzyme linked immunosorbent assay (ELISA) was carried out to measure the production of TNF-α and IL-12 in the sera of the immunized mice. The findings showed that the sera of rBCG without CRX immunised group yielding the most increase in IL-12 followed by the mice injected with rBCG with CRX. In contrast, the entire mice immunised groups showed decrease production for TNF-α after the immunisation. Therefore, this combination of rBCG with TLR4 agonist can be used to create a vaccine that capable of inducing a strong and long-lasting immunity to allow the immune system to be prepared for the emergence of multidrug-resistant strains of malaria diseases.
format	Thesis
qualification_level	Master's degree
author	Yusoff, Wan Raihan Wan
author_facet	Yusoff, Wan Raihan Wan
author_sort	Yusoff, Wan Raihan Wan
title	The effect of CRX-527 on pro-inflammatory cytokine production against mycobacterium bovis BCG clone expressing the c-terminus of merozoite surface protein-1 of plasmodium falciparum
title_short	The effect of CRX-527 on pro-inflammatory cytokine production against mycobacterium bovis BCG clone expressing the c-terminus of merozoite surface protein-1 of plasmodium falciparum
title_full	The effect of CRX-527 on pro-inflammatory cytokine production against mycobacterium bovis BCG clone expressing the c-terminus of merozoite surface protein-1 of plasmodium falciparum
title_fullStr	The effect of CRX-527 on pro-inflammatory cytokine production against mycobacterium bovis BCG clone expressing the c-terminus of merozoite surface protein-1 of plasmodium falciparum
title_full_unstemmed	The effect of CRX-527 on pro-inflammatory cytokine production against mycobacterium bovis BCG clone expressing the c-terminus of merozoite surface protein-1 of plasmodium falciparum
title_sort	effect of crx-527 on pro-inflammatory cytokine production against mycobacterium bovis bcg clone expressing the c-terminus of merozoite surface protein-1 of plasmodium falciparum
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kesihatan
publishDate	2021
url	http://eprints.usm.my/58306/1/Wan%20Raihan-24%20pages.pdf
_version_	1776101216053362688

The effect of CRX-527 on pro-inflammatory cytokine production against mycobacterium bovis BCG clone expressing the c-terminus of merozoite surface protein-1 of plasmodium falciparum

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