Molecular discrimination of bovine origin from processed canned products using PCR amplification of cytochrome B gene

The molecular method become the preferred technique used for the identification of animal species from processed meat products. PCR-based species-specific primers offer an advantage by targeting a specific species using a certain gene. Therefore, this study is aimed to identify beef meat content fro...

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Main Author:	Yu-Chen, Ng
Format:	Thesis
Language:	English
Published:	2022
Subjects:	QP501-801 Animal biochemistry
Online Access:	http://eprints.usm.my/58496/1/NG%20YU%20CHEN-24%20pages.pdf
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id	my-usm-ep.58496
record_format	uketd_dc
spelling	my-usm-ep.584962023-05-18T00:29:52Z Molecular discrimination of bovine origin from processed canned products using PCR amplification of cytochrome B gene 2022-09 Yu-Chen, Ng QP501-801 Animal biochemistry The molecular method become the preferred technique used for the identification of animal species from processed meat products. PCR-based species-specific primers offer an advantage by targeting a specific species using a certain gene. Therefore, this study is aimed to identify beef meat content from processed canned products around Kubang Kerian, Kelantan. DNA extraction of 12 canned products and one fresh raw beef meat was subjected to a DNeasy mericon food kit from Qiagen and quantification was performed using a Nanodrop spectrophotometer. PCR amplification using species-specific primer by targeting a mitochondrial DNA cytochrome b gene was amplified 120 bp of the target region. Success amplification was seen in the positive control sample (raw beef meat) and two canned products. Out of 12 processed canned products, 83.33% failed to detect or no DNA band was seen at 120 bp. This could be influenced by the manufacturing process for the canned products which involves high temperature and pressure treatment, hence affecting the genomic DNA to become degraded and/or possibly a very low amount of DNA was obtained from DNA extraction. In summary, the use of mitochondrial DNA markers specifically the cytochrome b gene has increased the possibility of the amplification of the targeted gene from processed canned products due to high copy number. 2022-09 Thesis http://eprints.usm.my/58496/ http://eprints.usm.my/58496/1/NG%20YU%20CHEN-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QP501-801 Animal biochemistry
spellingShingle	QP501-801 Animal biochemistry Yu-Chen, Ng Molecular discrimination of bovine origin from processed canned products using PCR amplification of cytochrome B gene
description	The molecular method become the preferred technique used for the identification of animal species from processed meat products. PCR-based species-specific primers offer an advantage by targeting a specific species using a certain gene. Therefore, this study is aimed to identify beef meat content from processed canned products around Kubang Kerian, Kelantan. DNA extraction of 12 canned products and one fresh raw beef meat was subjected to a DNeasy mericon food kit from Qiagen and quantification was performed using a Nanodrop spectrophotometer. PCR amplification using species-specific primer by targeting a mitochondrial DNA cytochrome b gene was amplified 120 bp of the target region. Success amplification was seen in the positive control sample (raw beef meat) and two canned products. Out of 12 processed canned products, 83.33% failed to detect or no DNA band was seen at 120 bp. This could be influenced by the manufacturing process for the canned products which involves high temperature and pressure treatment, hence affecting the genomic DNA to become degraded and/or possibly a very low amount of DNA was obtained from DNA extraction. In summary, the use of mitochondrial DNA markers specifically the cytochrome b gene has increased the possibility of the amplification of the targeted gene from processed canned products due to high copy number.
format	Thesis
qualification_level	Master's degree
author	Yu-Chen, Ng
author_facet	Yu-Chen, Ng
author_sort	Yu-Chen, Ng
title	Molecular discrimination of bovine origin from processed canned products using PCR amplification of cytochrome B gene
title_short	Molecular discrimination of bovine origin from processed canned products using PCR amplification of cytochrome B gene
title_full	Molecular discrimination of bovine origin from processed canned products using PCR amplification of cytochrome B gene
title_fullStr	Molecular discrimination of bovine origin from processed canned products using PCR amplification of cytochrome B gene
title_full_unstemmed	Molecular discrimination of bovine origin from processed canned products using PCR amplification of cytochrome B gene
title_sort	molecular discrimination of bovine origin from processed canned products using pcr amplification of cytochrome b gene
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kesihatan
publishDate	2022
url	http://eprints.usm.my/58496/1/NG%20YU%20CHEN-24%20pages.pdf
_version_	1776101224463990784

Molecular discrimination of bovine origin from processed canned products using PCR amplification of cytochrome B gene

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