PCR screening of genetically modified maize in biscuit products using TNOS gene

Recently, Genetically Modified (GM) plants are employed extensively in a variety of commercial goods. The use of advanced methods of genetic engineering in agriculture has led to an increase in the production of genetically modified crops as well as food products. However, the use of complex molecul...

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Main Author:	George, Sabrina
Format:	Thesis
Language:	English
Published:	2022
Subjects:	SB Plant culture
Online Access:	http://eprints.usm.my/58502/1/SABRINA%20GEORGE-24%20pages.pdf
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id	my-usm-ep.58502
record_format	uketd_dc
spelling	my-usm-ep.585022023-05-18T01:12:07Z PCR screening of genetically modified maize in biscuit products using TNOS gene 2022-09 George, Sabrina SB Plant culture Recently, Genetically Modified (GM) plants are employed extensively in a variety of commercial goods. The use of advanced methods of genetic engineering in agriculture has led to an increase in the production of genetically modified crops as well as food products. However, the use of complex molecular genetics technologies for their creation through nanobiotechnology manners to obtain genetically modified organisms, are not yet perfect, which causes a number of risks arising from the creation of such organisms. Maize is among the common genetically modified plants and has been integrated into the food and feed supply chains. In this study, the genetically modified TNOS gene was used to screen the biscuit samples bought from the market around Kota Bharu, Kelantan. DNeasy Mericon Food Kit was used to extract the biscuit samples, fresh maize, and GM maize positive control. PCR amplification was performed using zein and TNOS genes and results show all biscuit samples were absent of zein and TNOS genes. This could be caused by several reasons such as very low DNA concentration, the extracted DNA might be degraded, a different gene used for GM maize, the efficiency of conventional PCR and the sensitivity of agarose gel. Although the quantification reading for almost all samples showed an acceptable DNA concentration and good value for the first purity. Positive amplification for both genes was seen in the GM maize positive control. 2022-09 Thesis http://eprints.usm.my/58502/ http://eprints.usm.my/58502/1/SABRINA%20GEORGE-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	SB Plant culture
spellingShingle	SB Plant culture George, Sabrina PCR screening of genetically modified maize in biscuit products using TNOS gene
description	Recently, Genetically Modified (GM) plants are employed extensively in a variety of commercial goods. The use of advanced methods of genetic engineering in agriculture has led to an increase in the production of genetically modified crops as well as food products. However, the use of complex molecular genetics technologies for their creation through nanobiotechnology manners to obtain genetically modified organisms, are not yet perfect, which causes a number of risks arising from the creation of such organisms. Maize is among the common genetically modified plants and has been integrated into the food and feed supply chains. In this study, the genetically modified TNOS gene was used to screen the biscuit samples bought from the market around Kota Bharu, Kelantan. DNeasy Mericon Food Kit was used to extract the biscuit samples, fresh maize, and GM maize positive control. PCR amplification was performed using zein and TNOS genes and results show all biscuit samples were absent of zein and TNOS genes. This could be caused by several reasons such as very low DNA concentration, the extracted DNA might be degraded, a different gene used for GM maize, the efficiency of conventional PCR and the sensitivity of agarose gel. Although the quantification reading for almost all samples showed an acceptable DNA concentration and good value for the first purity. Positive amplification for both genes was seen in the GM maize positive control.
format	Thesis
qualification_level	Master's degree
author	George, Sabrina
author_facet	George, Sabrina
author_sort	George, Sabrina
title	PCR screening of genetically modified maize in biscuit products using TNOS gene
title_short	PCR screening of genetically modified maize in biscuit products using TNOS gene
title_full	PCR screening of genetically modified maize in biscuit products using TNOS gene
title_fullStr	PCR screening of genetically modified maize in biscuit products using TNOS gene
title_full_unstemmed	PCR screening of genetically modified maize in biscuit products using TNOS gene
title_sort	pcr screening of genetically modified maize in biscuit products using tnos gene
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kesihatan
publishDate	2022
url	http://eprints.usm.my/58502/1/SABRINA%20GEORGE-24%20pages.pdf
_version_	1776101225672998912

PCR screening of genetically modified maize in biscuit products using TNOS gene

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