Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation

Ludisia discolor is a jewel orchid that is well known for the striking leaves than its flowers. Other than being used as an ornamental plant, this orchid is also being exploited for its medicinal properties. Hence, L. discolor facing possible depletion in its natural habitat lately due to over-harve...

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Main Author:	Rajan, Kirutika Selva
Format:	Thesis
Language:	English
Published:	2022
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/59218/1/24%20Pages%20from%20KIRUTIKA%20AP%20SELVA%20RAJAN%20-%20TESIS.pdf
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id	my-usm-ep.59218
record_format	uketd_dc
spelling	my-usm-ep.592182023-08-21T08:00:09Z Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation 2022-03 Rajan, Kirutika Selva QH1-278.5 Natural history (General) Ludisia discolor is a jewel orchid that is well known for the striking leaves than its flowers. Other than being used as an ornamental plant, this orchid is also being exploited for its medicinal properties. Hence, L. discolor facing possible depletion in its natural habitat lately due to over-harvesting. Recently, the latest cryopreservation methods such as V cryo-plate is needed for long-term germplasm conservation. This study aims to develop a V cryo-plate cryopreservation method for L. discolor axillary buds to perform microscopy analysis of cryopreserved explants, determine the water content changes, and genetic stability between control and treated explants. Six (6) parameters have been optimized in this V cryo-plate method which are preculture concentration and duration, types of loading solution, duration on PVS2, thawing temperature, and thawing duration. 2022-03 Thesis http://eprints.usm.my/59218/ http://eprints.usm.my/59218/1/24%20Pages%20from%20KIRUTIKA%20AP%20SELVA%20RAJAN%20-%20TESIS.pdf application/pdf en public masters Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Rajan, Kirutika Selva Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation
description	Ludisia discolor is a jewel orchid that is well known for the striking leaves than its flowers. Other than being used as an ornamental plant, this orchid is also being exploited for its medicinal properties. Hence, L. discolor facing possible depletion in its natural habitat lately due to over-harvesting. Recently, the latest cryopreservation methods such as V cryo-plate is needed for long-term germplasm conservation. This study aims to develop a V cryo-plate cryopreservation method for L. discolor axillary buds to perform microscopy analysis of cryopreserved explants, determine the water content changes, and genetic stability between control and treated explants. Six (6) parameters have been optimized in this V cryo-plate method which are preculture concentration and duration, types of loading solution, duration on PVS2, thawing temperature, and thawing duration.
format	Thesis
qualification_level	Master's degree
author	Rajan, Kirutika Selva
author_facet	Rajan, Kirutika Selva
author_sort	Rajan, Kirutika Selva
title	Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation
title_short	Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation
title_full	Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation
title_fullStr	Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation
title_full_unstemmed	Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation
title_sort	development of v cryo-plate method of ludisia discolor (ker gawl.) a. rich for long-term germplasm conservation
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2022
url	http://eprints.usm.my/59218/1/24%20Pages%20from%20KIRUTIKA%20AP%20SELVA%20RAJAN%20-%20TESIS.pdf
_version_	1776101264881352704

Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation

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