Preliminary Study Of Agrobacterium-Mediated Transformation With Polyhydroxyalkanoate Genes For Rubber Tree Hevea Brasiliensis Muell. Arg. Clone Pb 350

Hevea brasiliensis (H. brasiliensis), better known as rubber tree, is the main producer of natural rubber. H. brasiliensis clone PB 350 is one of the clones recommended by the Malaysian Rubber Board (MRB) as a high yielding latex timber clone (LTC). This clone is a good candidate for heterologous pr...

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主要作者:	Lim, Kok Ming
格式:	Thesis
語言:	English
出版:	2022
主題:	QH1 Natural history (General - Including nature conservation geographical distribution)
在線閱讀:	http://eprints.usm.my/59401/1/LIM%20KOK%20MING%20-%20TESIS%20cut.pdf
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id	my-usm-ep.59401
record_format	uketd_dc
spelling	my-usm-ep.594012023-09-15T08:05:08Z Preliminary Study Of Agrobacterium-Mediated Transformation With Polyhydroxyalkanoate Genes For Rubber Tree Hevea Brasiliensis Muell. Arg. Clone Pb 350 2022-08 Lim, Kok Ming QH1 Natural history (General - Including nature conservation, geographical distribution) Hevea brasiliensis (H. brasiliensis), better known as rubber tree, is the main producer of natural rubber. H. brasiliensis clone PB 350 is one of the clones recommended by the Malaysian Rubber Board (MRB) as a high yielding latex timber clone (LTC). This clone is a good candidate for heterologous protein production because it can produce high amount of latex from where the heterologous protein could potentially be harvested from. Polyhydroxyalkanoate (PHA) is a biodegradable bioplastic produced by prokaryotes. While PHA has the potential to replace conventional plastics, the high cost of production makes it not feasible. Thus, attempts had been made to generate PHA-producing transgenic plants because carbon sources for heterologous protein production in plant comes from freely available carbon dioxide in the air and can potentially lower the production cost. Eventhough previous studies have reported on the transformation of H. brasiliensis, the transformation efficiency of different rubber clones differ. Therefore, the aim of this study was to determine suitable parameters to produce transgenic H. brasiliensis clone PB 350 capable of expressing the PHA biosynthetic genes. This study began with the screening for suitable explants for transformation. 2022-08 Thesis http://eprints.usm.my/59401/ http://eprints.usm.my/59401/1/LIM%20KOK%20MING%20-%20TESIS%20cut.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Lim, Kok Ming Preliminary Study Of Agrobacterium-Mediated Transformation With Polyhydroxyalkanoate Genes For Rubber Tree Hevea Brasiliensis Muell. Arg. Clone Pb 350
description	Hevea brasiliensis (H. brasiliensis), better known as rubber tree, is the main producer of natural rubber. H. brasiliensis clone PB 350 is one of the clones recommended by the Malaysian Rubber Board (MRB) as a high yielding latex timber clone (LTC). This clone is a good candidate for heterologous protein production because it can produce high amount of latex from where the heterologous protein could potentially be harvested from. Polyhydroxyalkanoate (PHA) is a biodegradable bioplastic produced by prokaryotes. While PHA has the potential to replace conventional plastics, the high cost of production makes it not feasible. Thus, attempts had been made to generate PHA-producing transgenic plants because carbon sources for heterologous protein production in plant comes from freely available carbon dioxide in the air and can potentially lower the production cost. Eventhough previous studies have reported on the transformation of H. brasiliensis, the transformation efficiency of different rubber clones differ. Therefore, the aim of this study was to determine suitable parameters to produce transgenic H. brasiliensis clone PB 350 capable of expressing the PHA biosynthetic genes. This study began with the screening for suitable explants for transformation.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Lim, Kok Ming
author_facet	Lim, Kok Ming
author_sort	Lim, Kok Ming
title	Preliminary Study Of Agrobacterium-Mediated Transformation With Polyhydroxyalkanoate Genes For Rubber Tree Hevea Brasiliensis Muell. Arg. Clone Pb 350
title_short	Preliminary Study Of Agrobacterium-Mediated Transformation With Polyhydroxyalkanoate Genes For Rubber Tree Hevea Brasiliensis Muell. Arg. Clone Pb 350
title_full	Preliminary Study Of Agrobacterium-Mediated Transformation With Polyhydroxyalkanoate Genes For Rubber Tree Hevea Brasiliensis Muell. Arg. Clone Pb 350
title_fullStr	Preliminary Study Of Agrobacterium-Mediated Transformation With Polyhydroxyalkanoate Genes For Rubber Tree Hevea Brasiliensis Muell. Arg. Clone Pb 350
title_full_unstemmed	Preliminary Study Of Agrobacterium-Mediated Transformation With Polyhydroxyalkanoate Genes For Rubber Tree Hevea Brasiliensis Muell. Arg. Clone Pb 350
title_sort	preliminary study of agrobacterium-mediated transformation with polyhydroxyalkanoate genes for rubber tree hevea brasiliensis muell. arg. clone pb 350
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2022
url	http://eprints.usm.my/59401/1/LIM%20KOK%20MING%20-%20TESIS%20cut.pdf
_version_	1783729549499957248

Preliminary Study Of Agrobacterium-Mediated Transformation With Polyhydroxyalkanoate Genes For Rubber Tree Hevea Brasiliensis Muell. Arg. Clone Pb 350

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