Response Of Bacillus Subtilis In Clonalcolony Competition

Competition is a common occurring in the natural environment due to the limitation of resources and spaces. Long term competition will eventually lead to stable coexistence. This research aims to investigate and establish the effect of clonal colony competition in Bacillus subtilis. This includes th...

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Main Author:	Law, Heng Chuan
Format:	Thesis
Language:	English
Published:	2022
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/59402/1/LAW%20HENG%20CHUAN%20-%20TESIS%20cut.pdf
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id	my-usm-ep.59402
record_format	uketd_dc
spelling	my-usm-ep.594022023-09-15T08:10:25Z Response Of Bacillus Subtilis In Clonalcolony Competition 2022-07 Law, Heng Chuan QH1 Natural history (General - Including nature conservation, geographical distribution) Competition is a common occurring in the natural environment due to the limitation of resources and spaces. Long term competition will eventually lead to stable coexistence. This research aims to investigate and establish the effect of clonal colony competition in Bacillus subtilis. This includes the physiological condition, viability status and transcriptomic response of the cell. When B. subtilis colonies are grown close together, growth occurs to a certain size with a distinct front, leaving a gap between them. Growth appeared to be arrested on the fronting side, while the side that did not face any other colony continued to grow outward and extend the morphology of the colony. Based on viability staining. the majority of the cells on the fronting sides of a 72-hour colony were either dead or undergoing changes to their cell membranes. Bacterial cells from the fronting side were observed to be sporulating by light microscopy, while cells on the outward growing side were unaffected. Transmission electron microscopy revealed that the cells on the fronting sides were mostly endospores, while the cells on the outward growing sides were mostly actively dividing vegetative cells with elongated nucleoplasmic body. A comparative transcriptomic analysis showed that the majority of the up-regulated genes in the cells on the front side are related to oxidative and nutrient stress. These distinct and highly up-regulated adaptive responses indicated that the cells were subjected to a variety of stress. 2022-07 Thesis http://eprints.usm.my/59402/ http://eprints.usm.my/59402/1/LAW%20HENG%20CHUAN%20-%20TESIS%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Law, Heng Chuan Response Of Bacillus Subtilis In Clonalcolony Competition
description	Competition is a common occurring in the natural environment due to the limitation of resources and spaces. Long term competition will eventually lead to stable coexistence. This research aims to investigate and establish the effect of clonal colony competition in Bacillus subtilis. This includes the physiological condition, viability status and transcriptomic response of the cell. When B. subtilis colonies are grown close together, growth occurs to a certain size with a distinct front, leaving a gap between them. Growth appeared to be arrested on the fronting side, while the side that did not face any other colony continued to grow outward and extend the morphology of the colony. Based on viability staining. the majority of the cells on the fronting sides of a 72-hour colony were either dead or undergoing changes to their cell membranes. Bacterial cells from the fronting side were observed to be sporulating by light microscopy, while cells on the outward growing side were unaffected. Transmission electron microscopy revealed that the cells on the fronting sides were mostly endospores, while the cells on the outward growing sides were mostly actively dividing vegetative cells with elongated nucleoplasmic body. A comparative transcriptomic analysis showed that the majority of the up-regulated genes in the cells on the front side are related to oxidative and nutrient stress. These distinct and highly up-regulated adaptive responses indicated that the cells were subjected to a variety of stress.
format	Thesis
qualification_level	Master's degree
author	Law, Heng Chuan
author_facet	Law, Heng Chuan
author_sort	Law, Heng Chuan
title	Response Of Bacillus Subtilis In Clonalcolony Competition
title_short	Response Of Bacillus Subtilis In Clonalcolony Competition
title_full	Response Of Bacillus Subtilis In Clonalcolony Competition
title_fullStr	Response Of Bacillus Subtilis In Clonalcolony Competition
title_full_unstemmed	Response Of Bacillus Subtilis In Clonalcolony Competition
title_sort	response of bacillus subtilis in clonalcolony competition
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2022
url	http://eprints.usm.my/59402/1/LAW%20HENG%20CHUAN%20-%20TESIS%20cut.pdf
_version_	1783729549753712640

Response Of Bacillus Subtilis In Clonalcolony Competition

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