Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation

Glucoamylase enzyme is widely utilized for conversion of starch into glucose during saccharification process. Aspergillus niger van Tieghem is commonly used for glucoamylase production in submerged cultivation system (SmF) and solid state fermentation (SSF). This research was carried out to enhan...

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Main Author:	Murugaiah, Hemalatha
Format:	Thesis
Language:	English
Published:	2012
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/59458/1/24%20Pages%20from%2000001760344.pdf
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id	my-usm-ep.59458
record_format	uketd_dc
spelling	my-usm-ep.594582023-10-05T04:16:10Z Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation 2012-07 Murugaiah, Hemalatha QH1-278.5 Natural history (General) Glucoamylase enzyme is widely utilized for conversion of starch into glucose during saccharification process. Aspergillus niger van Tieghem is commonly used for glucoamylase production in submerged cultivation system (SmF) and solid state fermentation (SSF). This research was carried out to enhance glucoamylase production by mutating A. niger van Tieghem using chemical mutagen, ethyl methane sulphonate (EMS) via random mutation. Mutant ANC9 was identified as a potential mutant which produced, 1.9 and 1.6 fold higher glucoamylase activity towards cooked starch and raw starch respectively in SmF using cooked starch as substrate. Glucoamylase activity towards raw starch and cooked starch in SSF by mutant ANC9 were 44.81± 1.2 Ulg and 101.50 ±2.2 Ulg respectively compared to wild strain which was only able to produce 34.4±O.5 Ulg towards cooked starch and 79.1±2.2 Ulg towards raw starch. 2012-07 Thesis http://eprints.usm.my/59458/ http://eprints.usm.my/59458/1/24%20Pages%20from%2000001760344.pdf application/pdf en public masters Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Murugaiah, Hemalatha Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation
description	Glucoamylase enzyme is widely utilized for conversion of starch into glucose during saccharification process. Aspergillus niger van Tieghem is commonly used for glucoamylase production in submerged cultivation system (SmF) and solid state fermentation (SSF). This research was carried out to enhance glucoamylase production by mutating A. niger van Tieghem using chemical mutagen, ethyl methane sulphonate (EMS) via random mutation. Mutant ANC9 was identified as a potential mutant which produced, 1.9 and 1.6 fold higher glucoamylase activity towards cooked starch and raw starch respectively in SmF using cooked starch as substrate. Glucoamylase activity towards raw starch and cooked starch in SSF by mutant ANC9 were 44.81± 1.2 Ulg and 101.50 ±2.2 Ulg respectively compared to wild strain which was only able to produce 34.4±O.5 Ulg towards cooked starch and 79.1±2.2 Ulg towards raw starch.
format	Thesis
qualification_level	Master's degree
author	Murugaiah, Hemalatha
author_facet	Murugaiah, Hemalatha
author_sort	Murugaiah, Hemalatha
title	Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation
title_short	Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation
title_full	Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation
title_fullStr	Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation
title_full_unstemmed	Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation
title_sort	strain improvement via random mutation for glucoamylase production by aspergillus niger van tieghem through solid state fermentation
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2012
url	http://eprints.usm.my/59458/1/24%20Pages%20from%2000001760344.pdf
_version_	1783729560789975040

Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation

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