Antiaging Activity Of Polyphenol Rich Calophyllum Inophyllum Fruit Extract In Aging Mutants Of S. Cerevisiae

Calophyllum inophyllum L. is an important medicinal plant with many ethnomedicinal values and exhibited significant anti-oxidant activity. Hence, this study was conducted to determine the in vitro anti-aging property of C. inophyllum fruit extract (CIFE) against Saccharomyces cerevisiae BY611 Strain...

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Main Author:	S. Venugopal, Kavilasha
Format:	Thesis
Language:	English
Published:	2022
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/59545/1/24%20Pages%20from%20KAVILASHA%20AP%20S.VENUGOPAL.pdf
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id	my-usm-ep.59545
record_format	uketd_dc
spelling	my-usm-ep.595452023-10-25T03:33:29Z Antiaging Activity Of Polyphenol Rich Calophyllum Inophyllum Fruit Extract In Aging Mutants Of S. Cerevisiae 2022-12 S. Venugopal, Kavilasha R5-920 Medicine (General) Calophyllum inophyllum L. is an important medicinal plant with many ethnomedicinal values and exhibited significant anti-oxidant activity. Hence, this study was conducted to determine the in vitro anti-aging property of C. inophyllum fruit extract (CIFE) against Saccharomyces cerevisiae BY611 Strain with the molecular mechanism of the anti-aging process. The anti-aging effect of CIFE against the yeast was evaluated through chronological lifespan assay, anti-oxidative stress assays, real-time quantitative polymerase chain reaction (RT-PCR) to study the regulation of superoxide dismutase (sod) and sirtuin 1 (sirt1) genes and superoxide dismutase enzyme (SOD) and sirtuin 1(SIRT1) protein activity assays were conducted at 1 mg/mL of CIFE. After administrating CIFE, the lifespan of the yeast was significantly prolonged in comparison with the untreated group (P<0.05) and also increases the yeast cell viability under oxidative stress by 4 mM H₂O₂ by spot assay. The Phloxine B stain method also further proves yeast cells' viability increased under oxidative stress by CIFE treatment. The reactive oxygen species (ROS) assay revealed that CIFE notably reduces the oxidative stress in treated yeast cells (P<0.05) comparing to untreated yeast cells. 2022-12 Thesis http://eprints.usm.my/59545/ http://eprints.usm.my/59545/1/24%20Pages%20from%20KAVILASHA%20AP%20S.VENUGOPAL.pdf application/pdf en public masters Perpustakaan Hamzah Sendut Institut Penyelidikan Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) S. Venugopal, Kavilasha Antiaging Activity Of Polyphenol Rich Calophyllum Inophyllum Fruit Extract In Aging Mutants Of S. Cerevisiae
description	Calophyllum inophyllum L. is an important medicinal plant with many ethnomedicinal values and exhibited significant anti-oxidant activity. Hence, this study was conducted to determine the in vitro anti-aging property of C. inophyllum fruit extract (CIFE) against Saccharomyces cerevisiae BY611 Strain with the molecular mechanism of the anti-aging process. The anti-aging effect of CIFE against the yeast was evaluated through chronological lifespan assay, anti-oxidative stress assays, real-time quantitative polymerase chain reaction (RT-PCR) to study the regulation of superoxide dismutase (sod) and sirtuin 1 (sirt1) genes and superoxide dismutase enzyme (SOD) and sirtuin 1(SIRT1) protein activity assays were conducted at 1 mg/mL of CIFE. After administrating CIFE, the lifespan of the yeast was significantly prolonged in comparison with the untreated group (P<0.05) and also increases the yeast cell viability under oxidative stress by 4 mM H₂O₂ by spot assay. The Phloxine B stain method also further proves yeast cells' viability increased under oxidative stress by CIFE treatment. The reactive oxygen species (ROS) assay revealed that CIFE notably reduces the oxidative stress in treated yeast cells (P<0.05) comparing to untreated yeast cells.
format	Thesis
qualification_level	Master's degree
author	S. Venugopal, Kavilasha
author_facet	S. Venugopal, Kavilasha
author_sort	S. Venugopal, Kavilasha
title	Antiaging Activity Of Polyphenol Rich Calophyllum Inophyllum Fruit Extract In Aging Mutants Of S. Cerevisiae
title_short	Antiaging Activity Of Polyphenol Rich Calophyllum Inophyllum Fruit Extract In Aging Mutants Of S. Cerevisiae
title_full	Antiaging Activity Of Polyphenol Rich Calophyllum Inophyllum Fruit Extract In Aging Mutants Of S. Cerevisiae
title_fullStr	Antiaging Activity Of Polyphenol Rich Calophyllum Inophyllum Fruit Extract In Aging Mutants Of S. Cerevisiae
title_full_unstemmed	Antiaging Activity Of Polyphenol Rich Calophyllum Inophyllum Fruit Extract In Aging Mutants Of S. Cerevisiae
title_sort	antiaging activity of polyphenol rich calophyllum inophyllum fruit extract in aging mutants of s. cerevisiae
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Institut Penyelidikan Perubatan Molekul
publishDate	2022
url	http://eprints.usm.my/59545/1/24%20Pages%20from%20KAVILASHA%20AP%20S.VENUGOPAL.pdf
_version_	1783729574817824768

Antiaging Activity Of Polyphenol Rich Calophyllum Inophyllum Fruit Extract In Aging Mutants Of S. Cerevisiae

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