Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells
Astaxanthin is a carotenoid with multiple health benefits including antioxidant and anticancer properties. An astaxanthin-hyperproducing X. dendrorhous mutant was generated due to the growing preference for natural pigment. This work aimed to study the astaxanthin-rich X. dendrorhous extracts and...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2022
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/59551/1/24%20Pages%20from%20KHAW%20SHIN%20YUAN.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Astaxanthin is a carotenoid with multiple health benefits including antioxidant
and anticancer properties. An astaxanthin-hyperproducing X. dendrorhous mutant was
generated due to the growing preference for natural pigment. This work aimed to study
the astaxanthin-rich X. dendrorhous extracts and its effect on MCF-7 and MDA-MB-
231 cells. Spectrometric, TLC and HPLC analysis evidenced that mutant M34
produced astaxanthin in higher quantity and purity than the wild type. Mutant
astaxanthin extract showed a higher scavenging activity compared to wild type
astaxanthin extract in DPPH assay. MTT assays proved that wild type and M34
astaxanthin extracts were not toxic towards the non-cancer origin MCF-10A cells.
Wild type and mutant astaxanthin extracts exhibit growth-inhibitory effect in dosedependent
manner in MCF-7 and MDA-MB-231 cells. Mutant astaxanthin extract
showed a lower IC50 than wild type extract and was more effective in inhibiting both
MCF-7 and MDA-MB-231 cells. The IC50 values of wild type and mutant astaxanthin
extracts did not exceed the required upper limit of 30 μg/ml. |
|---|