Chemopreventive Effects And Proteomic Analysis Of Myo Inositol Treatment On Human Prostate Cancer Cell Line (Du-145)

Chemopreventive Effects And Proteomic Analysis Of Myo Inositol Treatment On Human Prostate Cancer Cell Line (Du-145)

Prostate cancer remains the second most frequent diagnosed cancer in men and is the third leading cause of cancer related death, despite many available treatment options. The inefficiency of existing therapies and their adverse effects have led scientists to seek new treatments for this disease. The...

Full description

Saved in:
Bibliographic Details
Main Author: Islam, Mohammad Jahidul
Format: Thesis
Language:English
Published: 2022
Subjects:
R5-920 Medicine (General)
Online Access:http://eprints.usm.my/59555/1/24%20Pages%20from%20MOHAMMAD%20JAHIDUL%20ISLAM.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-usm-ep.59555
record_format uketd_dc
spelling my-usm-ep.595552023-10-26T02:41:46Z Chemopreventive Effects And Proteomic Analysis Of Myo Inositol Treatment On Human Prostate Cancer Cell Line (Du-145) 2022-02 Islam, Mohammad Jahidul R5-920 Medicine (General) Prostate cancer remains the second most frequent diagnosed cancer in men and is the third leading cause of cancer related death, despite many available treatment options. The inefficiency of existing therapies and their adverse effects have led scientists to seek new treatments for this disease. The identification and development of new anti-proliferative agents for prostate cancer is a major innovation in treating this deadly disease. Although the synthetic compound Myo-inositol is widely used in cancer research, the actual anti-cancer function of these chemicals is still not well understood. This study was therefore intended to unveil the chemopreventive effects of Myo-inositol on prostate cancer cells (DU-145). The growth inhibitory effect and the IC50 value were demonstrated by Myo-inositol at 0.06 mg/ml (**p<0.05). It is suspected that cell deaths are related to apoptosis induction and cell-cycle arrest. Treatment with Myo-inositol has been further identified by induction of early and late apoptosis (***p<0.01). Apoptosis can also be detected using DNA fragmentation and Hoechst 33342 fluorescent dye stain analysis. Myo-inositol caused an alteration to the cell cycle regulation on DU-145 cell line at G0/G1 and S phase, respectively (***p<0.01). Protein identification results via 2D electrophoresis and LC-MS/MS analysis showed 19 and 23 proteins identified in control and Myo-inositol treated samples, respectively. 2022-02 Thesis http://eprints.usm.my/59555/ http://eprints.usm.my/59555/1/24%20Pages%20from%20MOHAMMAD%20JAHIDUL%20ISLAM.pdf application/pdf en public phd doctoral Perpustakaan Hamzah Sendut Institut Perubatan & Pergigian Termaju
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic R5-920 Medicine (General)
spellingShingle R5-920 Medicine (General)
Islam, Mohammad Jahidul
Chemopreventive Effects And Proteomic Analysis Of Myo Inositol Treatment On Human Prostate Cancer Cell Line (Du-145)
description Prostate cancer remains the second most frequent diagnosed cancer in men and is the third leading cause of cancer related death, despite many available treatment options. The inefficiency of existing therapies and their adverse effects have led scientists to seek new treatments for this disease. The identification and development of new anti-proliferative agents for prostate cancer is a major innovation in treating this deadly disease. Although the synthetic compound Myo-inositol is widely used in cancer research, the actual anti-cancer function of these chemicals is still not well understood. This study was therefore intended to unveil the chemopreventive effects of Myo-inositol on prostate cancer cells (DU-145). The growth inhibitory effect and the IC50 value were demonstrated by Myo-inositol at 0.06 mg/ml (**p<0.05). It is suspected that cell deaths are related to apoptosis induction and cell-cycle arrest. Treatment with Myo-inositol has been further identified by induction of early and late apoptosis (***p<0.01). Apoptosis can also be detected using DNA fragmentation and Hoechst 33342 fluorescent dye stain analysis. Myo-inositol caused an alteration to the cell cycle regulation on DU-145 cell line at G0/G1 and S phase, respectively (***p<0.01). Protein identification results via 2D electrophoresis and LC-MS/MS analysis showed 19 and 23 proteins identified in control and Myo-inositol treated samples, respectively.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Islam, Mohammad Jahidul
author_facet Islam, Mohammad Jahidul
author_sort Islam, Mohammad Jahidul
title Chemopreventive Effects And Proteomic Analysis Of Myo Inositol Treatment On Human Prostate Cancer Cell Line (Du-145)
title_short Chemopreventive Effects And Proteomic Analysis Of Myo Inositol Treatment On Human Prostate Cancer Cell Line (Du-145)
title_full Chemopreventive Effects And Proteomic Analysis Of Myo Inositol Treatment On Human Prostate Cancer Cell Line (Du-145)
title_fullStr Chemopreventive Effects And Proteomic Analysis Of Myo Inositol Treatment On Human Prostate Cancer Cell Line (Du-145)
title_full_unstemmed Chemopreventive Effects And Proteomic Analysis Of Myo Inositol Treatment On Human Prostate Cancer Cell Line (Du-145)
title_sort chemopreventive effects and proteomic analysis of myo inositol treatment on human prostate cancer cell line (du-145)
granting_institution Perpustakaan Hamzah Sendut
granting_department Institut Perubatan & Pergigian Termaju
publishDate 2022
url http://eprints.usm.my/59555/1/24%20Pages%20from%20MOHAMMAD%20JAHIDUL%20ISLAM.pdf
_version_ 1783729577238986752

Similar Items