Development And Validation Of Online Preconcentration Capillary Electrophoresis Methods For The Determination Of Selected Pharmaceutical Compounds In Aqueous Matrices

In this study, several preconcentration strategies in capillary electrophoresis (CE) were developed to improve the sensitivity of detection of pharmaceutical compound in real aqueous matrices. This is due to the core challenge to develop simple and sensitive analytical method for trace analysis stud...

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Main Author:	Semail, Nadhiratul-Farihin
Format:	Thesis
Language:	English
Published:	2022
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/59608/1/24%20Pages%20from%20NADHIRATUL%20FARIHIN%20BINTI%20SEMAIL.pdf
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spelling	my-usm-ep.596082023-11-30T02:28:10Z Development And Validation Of Online Preconcentration Capillary Electrophoresis Methods For The Determination Of Selected Pharmaceutical Compounds In Aqueous Matrices 2022-01 Semail, Nadhiratul-Farihin R5-920 Medicine (General) In this study, several preconcentration strategies in capillary electrophoresis (CE) were developed to improve the sensitivity of detection of pharmaceutical compound in real aqueous matrices. This is due to the core challenge to develop simple and sensitive analytical method for trace analysis study of selected analytes (anticancer, antibiotic and non-steroidal anti-inflammatory drugs) are very limited. Higher interference level from complex matrix (biological, food and water) with analytical instrument poor detection capability directed to development of online preconcentration technique. The first technique termed sequential injection mode stacking was developed for the separation and determination of anticancer agent 5-fluorouracil (5-FU) and its metabolite namely, 5-fluoro-2-deoxyuridine (5-FdUrd) in human plasma obtained from cancer patients. In this technique, large sample volume was introduced through electrokinetic injection followed by hydrodynamic injection. Under the optimized experimental conditions, 614- and 643- fold and 782- and 803- fold sensitivity improvement was obtained for both 5-FU and its metabolite when compared with normal hydrodynamic and electrokinetic injection alone, respectively. 2022-01 Thesis http://eprints.usm.my/59608/ http://eprints.usm.my/59608/1/24%20Pages%20from%20NADHIRATUL%20FARIHIN%20BINTI%20SEMAIL.pdf application/pdf en public phd doctoral Perpustakaan Hamzah Sendut Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Semail, Nadhiratul-Farihin Development And Validation Of Online Preconcentration Capillary Electrophoresis Methods For The Determination Of Selected Pharmaceutical Compounds In Aqueous Matrices
description	In this study, several preconcentration strategies in capillary electrophoresis (CE) were developed to improve the sensitivity of detection of pharmaceutical compound in real aqueous matrices. This is due to the core challenge to develop simple and sensitive analytical method for trace analysis study of selected analytes (anticancer, antibiotic and non-steroidal anti-inflammatory drugs) are very limited. Higher interference level from complex matrix (biological, food and water) with analytical instrument poor detection capability directed to development of online preconcentration technique. The first technique termed sequential injection mode stacking was developed for the separation and determination of anticancer agent 5-fluorouracil (5-FU) and its metabolite namely, 5-fluoro-2-deoxyuridine (5-FdUrd) in human plasma obtained from cancer patients. In this technique, large sample volume was introduced through electrokinetic injection followed by hydrodynamic injection. Under the optimized experimental conditions, 614- and 643- fold and 782- and 803- fold sensitivity improvement was obtained for both 5-FU and its metabolite when compared with normal hydrodynamic and electrokinetic injection alone, respectively.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Semail, Nadhiratul-Farihin
author_facet	Semail, Nadhiratul-Farihin
author_sort	Semail, Nadhiratul-Farihin
title	Development And Validation Of Online Preconcentration Capillary Electrophoresis Methods For The Determination Of Selected Pharmaceutical Compounds In Aqueous Matrices
title_short	Development And Validation Of Online Preconcentration Capillary Electrophoresis Methods For The Determination Of Selected Pharmaceutical Compounds In Aqueous Matrices
title_full	Development And Validation Of Online Preconcentration Capillary Electrophoresis Methods For The Determination Of Selected Pharmaceutical Compounds In Aqueous Matrices
title_fullStr	Development And Validation Of Online Preconcentration Capillary Electrophoresis Methods For The Determination Of Selected Pharmaceutical Compounds In Aqueous Matrices
title_full_unstemmed	Development And Validation Of Online Preconcentration Capillary Electrophoresis Methods For The Determination Of Selected Pharmaceutical Compounds In Aqueous Matrices
title_sort	development and validation of online preconcentration capillary electrophoresis methods for the determination of selected pharmaceutical compounds in aqueous matrices
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2022
url	http://eprints.usm.my/59608/1/24%20Pages%20from%20NADHIRATUL%20FARIHIN%20BINTI%20SEMAIL.pdf
_version_	1794024026600898560

Development And Validation Of Online Preconcentration Capillary Electrophoresis Methods For The Determination Of Selected Pharmaceutical Compounds In Aqueous Matrices

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