Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets
As a monopodial plant, Phalaenopsis is traditionally propagated by off-shoot cutting or division. However, this resulted in a low multiplication rate and hinders the growth of the mother plant and therefore inefficient for large-scale production. Micropropagation has provided a valuable alternati...
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| 主要作者: | |
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| 格式: | Thesis |
| 語言: | English |
| 出版: |
2022
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| 主題: | |
| 在線閱讀: | http://eprints.usm.my/59667/1/24%20Pages%20from%20ARULVILEE%20AP%20RAJASEGAR%20-%20TESIS.pdf |
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| 總結: | As a monopodial plant, Phalaenopsis is traditionally propagated by off-shoot
cutting or division. However, this resulted in a low multiplication rate and hinders
the growth of the mother plant and therefore inefficient for large-scale production.
Micropropagation has provided a valuable alternative method for the large-scale
production of identical orchid plantlets within a short period. The study aimed to
improve the proliferation of protocorm-like bodies (PLBs) of Phalaenopsis
Fuller’s Light × Phalaenopsis Purple Heart through the micropropagation method.
The thin cell layer culture (TCL) approach generated a 2.16 times higher neo-PLB
formation rate than the conventional half-PLB explants. The medium composition
of full MS medium supplemented with 3% sucrose, 2% banana extract, and 15%
coconutwater significantly produced the highest PLB fresh weight (2.499 g) after
4 weeks of culture. The Phalaenopsis PLBs further developed into shoot and root
using 1.0 mg/L BAP and NAA mg/L. The incorporation of 1.5 mg/L of glutamine
resulted shoot formation with 70% while 1.5 mg/L of arginine increased root
formation by 87%, wherein the cultures were differentiated into complete
plantlets. |
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