Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets

As a monopodial plant, Phalaenopsis is traditionally propagated by off-shoot cutting or division. However, this resulted in a low multiplication rate and hinders the growth of the mother plant and therefore inefficient for large-scale production. Micropropagation has provided a valuable alternati...

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Main Author:	Rajasegar, Arulvilee
Format:	Thesis
Language:	English
Published:	2022
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/59667/1/24%20Pages%20from%20ARULVILEE%20AP%20RAJASEGAR%20-%20TESIS.pdf
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id	my-usm-ep.59667
record_format	uketd_dc
spelling	my-usm-ep.596672023-12-13T01:28:02Z Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets 2022-09 Rajasegar, Arulvilee QH1-278.5 Natural history (General) As a monopodial plant, Phalaenopsis is traditionally propagated by off-shoot cutting or division. However, this resulted in a low multiplication rate and hinders the growth of the mother plant and therefore inefficient for large-scale production. Micropropagation has provided a valuable alternative method for the large-scale production of identical orchid plantlets within a short period. The study aimed to improve the proliferation of protocorm-like bodies (PLBs) of Phalaenopsis Fuller’s Light × Phalaenopsis Purple Heart through the micropropagation method. The thin cell layer culture (TCL) approach generated a 2.16 times higher neo-PLB formation rate than the conventional half-PLB explants. The medium composition of full MS medium supplemented with 3% sucrose, 2% banana extract, and 15% coconutwater significantly produced the highest PLB fresh weight (2.499 g) after 4 weeks of culture. The Phalaenopsis PLBs further developed into shoot and root using 1.0 mg/L BAP and NAA mg/L. The incorporation of 1.5 mg/L of glutamine resulted shoot formation with 70% while 1.5 mg/L of arginine increased root formation by 87%, wherein the cultures were differentiated into complete plantlets. 2022-09 Thesis http://eprints.usm.my/59667/ http://eprints.usm.my/59667/1/24%20Pages%20from%20ARULVILEE%20AP%20RAJASEGAR%20-%20TESIS.pdf application/pdf en public phd doctoral Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Rajasegar, Arulvilee Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets
description	As a monopodial plant, Phalaenopsis is traditionally propagated by off-shoot cutting or division. However, this resulted in a low multiplication rate and hinders the growth of the mother plant and therefore inefficient for large-scale production. Micropropagation has provided a valuable alternative method for the large-scale production of identical orchid plantlets within a short period. The study aimed to improve the proliferation of protocorm-like bodies (PLBs) of Phalaenopsis Fuller’s Light × Phalaenopsis Purple Heart through the micropropagation method. The thin cell layer culture (TCL) approach generated a 2.16 times higher neo-PLB formation rate than the conventional half-PLB explants. The medium composition of full MS medium supplemented with 3% sucrose, 2% banana extract, and 15% coconutwater significantly produced the highest PLB fresh weight (2.499 g) after 4 weeks of culture. The Phalaenopsis PLBs further developed into shoot and root using 1.0 mg/L BAP and NAA mg/L. The incorporation of 1.5 mg/L of glutamine resulted shoot formation with 70% while 1.5 mg/L of arginine increased root formation by 87%, wherein the cultures were differentiated into complete plantlets.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Rajasegar, Arulvilee
author_facet	Rajasegar, Arulvilee
author_sort	Rajasegar, Arulvilee
title	Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets
title_short	Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets
title_full	Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets
title_fullStr	Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets
title_full_unstemmed	Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets
title_sort	development of an efficient protocol for the production of phalaenopsis hybrid orchid plantlets
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2022
url	http://eprints.usm.my/59667/1/24%20Pages%20from%20ARULVILEE%20AP%20RAJASEGAR%20-%20TESIS.pdf
_version_	1794024030751162368

Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets

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