Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis

Mycobacterium tuberculosis is an actinobacterium that causes chronic infections. Its pathogenesis lies in the thick, lipid-rich cell envelope that facilitate persistence in hostile environments. A refined knowledge of regulatory networks within M. tuberculosis is important to understand its patho...

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Main Author:	Cheah, Hong Leong
Format:	Thesis
Language:	English
Published:	2022
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/59681/1/24%20Pages%20from%20CHEAH%20HONG%20LEONG%20-%20TESIS.pdf
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id	my-usm-ep.59681
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spelling	my-usm-ep.596812023-12-14T01:49:49Z Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis 2022-09 Cheah, Hong Leong R5-920 Medicine (General) Mycobacterium tuberculosis is an actinobacterium that causes chronic infections. Its pathogenesis lies in the thick, lipid-rich cell envelope that facilitate persistence in hostile environments. A refined knowledge of regulatory networks within M. tuberculosis is important to understand its pathogenesis for therapeutic design. Small regulatory RNAs (sRNAs) are key modulatory components within all organisms. Extensive studies over the past decade have uncovered a plethora of bacterial sRNAs, implying that bacterial transcriptomes are far more sophisticated than previously anticipated. Here, sRNA profiles of M. tuberculosis exposed to different stresses were established by analyzing RNA-seq data to address different facets of sRNAome in M. tuberculosis. This study has identified a total of 1,376 sRNA candidates and the subsequent 5’/3’RACE assay has successfully validated a total of five sRNAs, among which predictedRNA_0578 may encode for a novel small peptide and predictedRNA_0020 potentially affects the formation of mycobacterial cell wall. Besides, this study also included transcription start site (TSS) landscapes that provide additional transcriptional features, e.g., promoters and untranslated regions (UTRs). Taken together, this study reveals the complexities of the mycobacterial sRNAome and may facilitate the development of antisense antibiotics. 2022-09 Thesis http://eprints.usm.my/59681/ http://eprints.usm.my/59681/1/24%20Pages%20from%20CHEAH%20HONG%20LEONG%20-%20TESIS.pdf application/pdf en public phd doctoral Perpustakaan Hamzah Sendut Institut Perubatan dan Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Cheah, Hong Leong Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis
description	Mycobacterium tuberculosis is an actinobacterium that causes chronic infections. Its pathogenesis lies in the thick, lipid-rich cell envelope that facilitate persistence in hostile environments. A refined knowledge of regulatory networks within M. tuberculosis is important to understand its pathogenesis for therapeutic design. Small regulatory RNAs (sRNAs) are key modulatory components within all organisms. Extensive studies over the past decade have uncovered a plethora of bacterial sRNAs, implying that bacterial transcriptomes are far more sophisticated than previously anticipated. Here, sRNA profiles of M. tuberculosis exposed to different stresses were established by analyzing RNA-seq data to address different facets of sRNAome in M. tuberculosis. This study has identified a total of 1,376 sRNA candidates and the subsequent 5’/3’RACE assay has successfully validated a total of five sRNAs, among which predictedRNA_0578 may encode for a novel small peptide and predictedRNA_0020 potentially affects the formation of mycobacterial cell wall. Besides, this study also included transcription start site (TSS) landscapes that provide additional transcriptional features, e.g., promoters and untranslated regions (UTRs). Taken together, this study reveals the complexities of the mycobacterial sRNAome and may facilitate the development of antisense antibiotics.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Cheah, Hong Leong
author_facet	Cheah, Hong Leong
author_sort	Cheah, Hong Leong
title	Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis
title_short	Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis
title_full	Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis
title_fullStr	Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis
title_full_unstemmed	Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis
title_sort	transcription start site mapping and small regulatory rna profiling of mycobacterium tuberculosis
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Institut Perubatan dan Pergigian Termaju
publishDate	2022
url	http://eprints.usm.my/59681/1/24%20Pages%20from%20CHEAH%20HONG%20LEONG%20-%20TESIS.pdf
_version_	1794024033690320896

Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis

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