Fish Identification Across Merbok Estuary Through Dna Barcoding And Environmental Dna (Edna) Metabarcoding In Tropical Mangrove Estuary

Biodiversity hotspots often face a taxonomic information gap, especially those biotas found in tropical mangrove estuarine ecosystems. These megadiverse ecosystems are currently among the most threatened ecosystems in the world due to high human pressures. Moreover, knowledge of the taxonomy and dis...

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Main Author:	Zainal Abidin, Danial Hariz
Format:	Thesis
Language:	English
Published:	2022
Subjects:	HG2397-3550 By region or country
Online Access:	http://eprints.usm.my/59684/1/24%20Pages%20from%20DANIAL%20HARIZ%20BIN%20ZAINAL%20ABIDIN%20-%20TESIS.pdf
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id	my-usm-ep.59684
record_format	uketd_dc
spelling	my-usm-ep.596842023-12-15T02:11:06Z Fish Identification Across Merbok Estuary Through Dna Barcoding And Environmental Dna (Edna) Metabarcoding In Tropical Mangrove Estuary 2022-09 Zainal Abidin, Danial Hariz HG2397-3550 By region or country Biodiversity hotspots often face a taxonomic information gap, especially those biotas found in tropical mangrove estuarine ecosystems. These megadiverse ecosystems are currently among the most threatened ecosystems in the world due to high human pressures. Moreover, knowledge of the taxonomy and distribution of species in this ecosystem is still incomplete for many groups, especially fish taxa, which hinders the implementation of sustainable biodiversity conservation plans. To fill the taxonomic gap in this species-rich region, this work investigated the diversity of fish communities in a tropical mangrove estuary - the Merbok Estuary in northwestern Peninsular Malaysia using conventional and DNA-based methods. The conventional assessment of ichthyodiversity in the Merbok Estuary and adjacent marine waters recorded a total of 138 fish species from two classes, 18 orders, 47 families, and 94 genera. A repository of specimens was established at the Biodiversity Reference Laboratory, USM, to enable further comparative studies. Subsequently, DNA barcoding based on the COI gene was used to genetically assess the fish diversity in the Merbok Estuary and complement the earlier morphological results. A DNA barcoding reference library of 350 fish individuals was compiled. Of the 138 species initially identified by morphology, the barcodes of 123 species confirm their validity. 2022-09 Thesis http://eprints.usm.my/59684/ http://eprints.usm.my/59684/1/24%20Pages%20from%20DANIAL%20HARIZ%20BIN%20ZAINAL%20ABIDIN%20-%20TESIS.pdf application/pdf en public phd doctoral Perpustakaan Hamzah Sendut Pusat Kajian Kelestarian Global
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	HG2397-3550 By region or country
spellingShingle	HG2397-3550 By region or country Zainal Abidin, Danial Hariz Fish Identification Across Merbok Estuary Through Dna Barcoding And Environmental Dna (Edna) Metabarcoding In Tropical Mangrove Estuary
description	Biodiversity hotspots often face a taxonomic information gap, especially those biotas found in tropical mangrove estuarine ecosystems. These megadiverse ecosystems are currently among the most threatened ecosystems in the world due to high human pressures. Moreover, knowledge of the taxonomy and distribution of species in this ecosystem is still incomplete for many groups, especially fish taxa, which hinders the implementation of sustainable biodiversity conservation plans. To fill the taxonomic gap in this species-rich region, this work investigated the diversity of fish communities in a tropical mangrove estuary - the Merbok Estuary in northwestern Peninsular Malaysia using conventional and DNA-based methods. The conventional assessment of ichthyodiversity in the Merbok Estuary and adjacent marine waters recorded a total of 138 fish species from two classes, 18 orders, 47 families, and 94 genera. A repository of specimens was established at the Biodiversity Reference Laboratory, USM, to enable further comparative studies. Subsequently, DNA barcoding based on the COI gene was used to genetically assess the fish diversity in the Merbok Estuary and complement the earlier morphological results. A DNA barcoding reference library of 350 fish individuals was compiled. Of the 138 species initially identified by morphology, the barcodes of 123 species confirm their validity.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Zainal Abidin, Danial Hariz
author_facet	Zainal Abidin, Danial Hariz
author_sort	Zainal Abidin, Danial Hariz
title	Fish Identification Across Merbok Estuary Through Dna Barcoding And Environmental Dna (Edna) Metabarcoding In Tropical Mangrove Estuary
title_short	Fish Identification Across Merbok Estuary Through Dna Barcoding And Environmental Dna (Edna) Metabarcoding In Tropical Mangrove Estuary
title_full	Fish Identification Across Merbok Estuary Through Dna Barcoding And Environmental Dna (Edna) Metabarcoding In Tropical Mangrove Estuary
title_fullStr	Fish Identification Across Merbok Estuary Through Dna Barcoding And Environmental Dna (Edna) Metabarcoding In Tropical Mangrove Estuary
title_full_unstemmed	Fish Identification Across Merbok Estuary Through Dna Barcoding And Environmental Dna (Edna) Metabarcoding In Tropical Mangrove Estuary
title_sort	fish identification across merbok estuary through dna barcoding and environmental dna (edna) metabarcoding in tropical mangrove estuary
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Pusat Kajian Kelestarian Global
publishDate	2022
url	http://eprints.usm.my/59684/1/24%20Pages%20from%20DANIAL%20HARIZ%20BIN%20ZAINAL%20ABIDIN%20-%20TESIS.pdf
_version_	1794024034424324096

Fish Identification Across Merbok Estuary Through Dna Barcoding And Environmental Dna (Edna) Metabarcoding In Tropical Mangrove Estuary

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