Halochlorella Rubescens Cultivation In Photobioreactors For Optimum Lipid Production

Halochlorella Rubescens Cultivation In Photobioreactors For Optimum Lipid Production

Lipids from microalgae have been studied for their potential application in various industries. The microalgae lipid production is mainly influenced by the cultivation methods and environmental conditions. This study investigates the effects of lipid content and lipid formation kinetics at different...

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Main Author: Anbalagan, Lavanya
Format: Thesis
Language:English
Published: 2022
Subjects:
T1-995 Technology(General)
Online Access:http://eprints.usm.my/59704/1/24%20Pages%20from%20LAVANYA%20AP%20ANBALAGAN%20-%20TESIS.pdf
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spelling my-usm-ep.597042023-12-19T04:12:33Z Halochlorella Rubescens Cultivation In Photobioreactors For Optimum Lipid Production 2022-09 Anbalagan, Lavanya T1-995 Technology(General) Lipids from microalgae have been studied for their potential application in various industries. The microalgae lipid production is mainly influenced by the cultivation methods and environmental conditions. This study investigates the effects of lipid content and lipid formation kinetics at different cultivation conditions involving pH, combination of nitrogen and phosphorus sources of different concentrations, carbon dioxide concentrations and light intensity on Halochlorella rubescens, a freshwater microalgae in photobioreactors with different configurations such as the cubic tank photobioreactor (CTPBR), centralized light photobioreactor (CLPBR) and tubular photobioreactor (TPBR) using one variable at a time (OVAT) method. The significance of differences between each test variable was determined using one way ANOVA where (p ˂ 0.05) is considered statistically significant. Qualitatively the lipid accumulation of cells was determined via Nile red staining method. The analysis of Fourier Transform Infrared Spectroscopy (FTIR) at different parameters confirmed the functional groups of lipids. The Leudeking-Piret model revealed that the lipid production of Halochlorella rubescens is growth-associated at all different cultivation conditions. The highest lipid content (39.42 ± 0.426%) and maximum biomass density (0.3662 ± 0.002 gL-1) was achieved at optimized conditions (pH 9, nitrogen-depleted medium, 15% CO2, 4000 lux) in 10L volume using TPBR. 2022-09 Thesis http://eprints.usm.my/59704/ http://eprints.usm.my/59704/1/24%20Pages%20from%20LAVANYA%20AP%20ANBALAGAN%20-%20TESIS.pdf application/pdf en public masters Perpustakaan Hamzah Sendut Pusat Pengajian Teknologi Industri
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic T1-995 Technology(General)
spellingShingle T1-995 Technology(General)
Anbalagan, Lavanya
Halochlorella Rubescens Cultivation In Photobioreactors For Optimum Lipid Production
description Lipids from microalgae have been studied for their potential application in various industries. The microalgae lipid production is mainly influenced by the cultivation methods and environmental conditions. This study investigates the effects of lipid content and lipid formation kinetics at different cultivation conditions involving pH, combination of nitrogen and phosphorus sources of different concentrations, carbon dioxide concentrations and light intensity on Halochlorella rubescens, a freshwater microalgae in photobioreactors with different configurations such as the cubic tank photobioreactor (CTPBR), centralized light photobioreactor (CLPBR) and tubular photobioreactor (TPBR) using one variable at a time (OVAT) method. The significance of differences between each test variable was determined using one way ANOVA where (p ˂ 0.05) is considered statistically significant. Qualitatively the lipid accumulation of cells was determined via Nile red staining method. The analysis of Fourier Transform Infrared Spectroscopy (FTIR) at different parameters confirmed the functional groups of lipids. The Leudeking-Piret model revealed that the lipid production of Halochlorella rubescens is growth-associated at all different cultivation conditions. The highest lipid content (39.42 ± 0.426%) and maximum biomass density (0.3662 ± 0.002 gL-1) was achieved at optimized conditions (pH 9, nitrogen-depleted medium, 15% CO2, 4000 lux) in 10L volume using TPBR.
format Thesis
qualification_level Master's degree
author Anbalagan, Lavanya
author_facet Anbalagan, Lavanya
author_sort Anbalagan, Lavanya
title Halochlorella Rubescens Cultivation In Photobioreactors For Optimum Lipid Production
title_short Halochlorella Rubescens Cultivation In Photobioreactors For Optimum Lipid Production
title_full Halochlorella Rubescens Cultivation In Photobioreactors For Optimum Lipid Production
title_fullStr Halochlorella Rubescens Cultivation In Photobioreactors For Optimum Lipid Production
title_full_unstemmed Halochlorella Rubescens Cultivation In Photobioreactors For Optimum Lipid Production
title_sort halochlorella rubescens cultivation in photobioreactors for optimum lipid production
granting_institution Perpustakaan Hamzah Sendut
granting_department Pusat Pengajian Teknologi Industri
publishDate 2022
url http://eprints.usm.my/59704/1/24%20Pages%20from%20LAVANYA%20AP%20ANBALAGAN%20-%20TESIS.pdf
_version_ 1794024039427080192

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