Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing

Tapinoma indicum Forel (Hymenoptera: Formicidae) is a household pest that is widely distributed in Asian countries. It is known as a nuisance pest that causes annoyance and disturbance by constructing nests and foraging in building for food and water. However, studies on T. indicum are limited, e...

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主要作者: Lim, Li Yang
格式: Thesis
語言:English
出版: 2022
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spelling my-usm-ep.597062023-12-19T04:16:29Z Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing 2022-09 Lim, Li Yang QH1-278.5 Natural history (General) Tapinoma indicum Forel (Hymenoptera: Formicidae) is a household pest that is widely distributed in Asian countries. It is known as a nuisance pest that causes annoyance and disturbance by constructing nests and foraging in building for food and water. However, studies on T. indicum are limited, especially in the field of molecular biology. Hence, this research aims to study the T. indicum population genetic structure in Penang Island, Malaysia by using the novel microsatellite markers developed from whole genome of T. indium. Objectives of this research include: to determine the T. indicum population distribution in Penang island, Malaysia; to identify the phylogenetic relationship of T. indicum based on the mitochondrial gene of cytochrome c oxidase subunit Ⅰ (CO1) and 16S ribosomal DNA (16S rDNA); to design novel microsatellite marker by screening microsatellite loci of T. indicum genome sequenced from next-generation sequencing; to validate polymorphism of designed microsatellite marker; to analyse genetic diversity and population genetic structure of T. indicum using designed microsatellite markers. There was a total of 30 populations collected from Balik Pulau, Gelugor and George Town. The CO1 sequences and 16S rDNA sequences of T. indicum from each population were deposited and accessible via Genbank (NCBI) database. 2022-09 Thesis http://eprints.usm.my/59706/ http://eprints.usm.my/59706/1/24%20Pages%20from%20LIM%20LI%20YANG%20-%20TESIS.pdf application/pdf en public phd doctoral Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kajihayat
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic QH1-278.5 Natural history (General)
spellingShingle QH1-278.5 Natural history (General)
Lim, Li Yang
Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing
description Tapinoma indicum Forel (Hymenoptera: Formicidae) is a household pest that is widely distributed in Asian countries. It is known as a nuisance pest that causes annoyance and disturbance by constructing nests and foraging in building for food and water. However, studies on T. indicum are limited, especially in the field of molecular biology. Hence, this research aims to study the T. indicum population genetic structure in Penang Island, Malaysia by using the novel microsatellite markers developed from whole genome of T. indium. Objectives of this research include: to determine the T. indicum population distribution in Penang island, Malaysia; to identify the phylogenetic relationship of T. indicum based on the mitochondrial gene of cytochrome c oxidase subunit Ⅰ (CO1) and 16S ribosomal DNA (16S rDNA); to design novel microsatellite marker by screening microsatellite loci of T. indicum genome sequenced from next-generation sequencing; to validate polymorphism of designed microsatellite marker; to analyse genetic diversity and population genetic structure of T. indicum using designed microsatellite markers. There was a total of 30 populations collected from Balik Pulau, Gelugor and George Town. The CO1 sequences and 16S rDNA sequences of T. indicum from each population were deposited and accessible via Genbank (NCBI) database.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Lim, Li Yang
author_facet Lim, Li Yang
author_sort Lim, Li Yang
title Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing
title_short Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing
title_full Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing
title_fullStr Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing
title_full_unstemmed Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing
title_sort population genetic study of tapinoma indicum (forel) in penang island, malaysia using microsatellite marker developed by nextgeneration sequencing
granting_institution Perpustakaan Hamzah Sendut
granting_department Pusat Pengajian Sains Kajihayat
publishDate 2022
url http://eprints.usm.my/59706/1/24%20Pages%20from%20LIM%20LI%20YANG%20-%20TESIS.pdf
_version_ 1794024039914668032