Development of a novel bioceramic root canal sealer and its effect on the dislodgement resistance and dentinal tubule penetration

The present study aimed to fabricate and characterise novel algin-incorporated bioactive glass 58S calcium-silicate root canal sealer (Bio-G) and evaluate its effect on the dislodgement resistance and dentinal tubule penetration to root dentinal walls in comparison to commercially available bioce...

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Main Author:	Lin, Galvin Sim Siang
Format:	Thesis
Language:	English
Published:	2023
Subjects:	R Medicine RK1-715 Dentistry
Online Access:	http://eprints.usm.my/59823/1/GALVIN%20SIM%20SIANG%20LIN%20-%20FINAL%20THESIS%20P-SGD000321%28R%29-E.pdf
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spelling	my-usm-ep.598232024-02-13T07:44:05Z Development of a novel bioceramic root canal sealer and its effect on the dislodgement resistance and dentinal tubule penetration 2023-06 Lin, Galvin Sim Siang R Medicine RK1-715 Dentistry The present study aimed to fabricate and characterise novel algin-incorporated bioactive glass 58S calcium-silicate root canal sealer (Bio-G) and evaluate its effect on the dislodgement resistance and dentinal tubule penetration to root dentinal walls in comparison to commercially available bioceramic-based sealers (BioRoot RCS and iRoot SP). Three groups of bioactive glass (BG) 58S powders were synthesised using sol-gel method and characterised using SEM, HRTEM and FTIR for optimisation: BG-1 (no ammonia), BG-2 (3 ml of ammonia), and BG-3 (5 ml of ammonia). Optimised BG-3 was used to fabricate the powder form of experimental Bio-G sealer with the addition of calcium silicate, zirconia dioxide, calcium carbonate and alginic acid powder. Meanwhile, the liquid form composed of 5% calcium chloride solution. The experimental 0-5% algin Bio-G sealers were then compared with BioRoot RCS and iRoot SP. Standardised disc specimens of mixed sealer materials (n=5 per group) were prepared and placed in an incubator to allow setting before characterising under SEM, HRTEM, EDS, FTIR and XRD. Next, one-hundred-and-seventy-six mandibular premolars were randomly assigned (n=16 per group): control, gutta-percha + 0-5% algin Bio-G, gutta-percha + BioRoot RCS, and gutta-percha + iRoot SP groups, with the exclusion of the control group in adhesive pattern and dentinal tubule penetration tests. They were instrumented, obturated and placed in an incubator for 72 hours to allow sealer set. For the dentinal tubule penetration test, sealers were mixed with 0.1% of rhodamine B dye. Subsequently, teeth were cut into a 1-mm-thick cross-section at 5-mm and 10-mm levels from the root apex, respectively. Push-out bond strength, adhesive pattern and dentinal tubule penetration tests were performed. Experimental Bio-G sealer revealed irregular micro-sized particles with a higher content of oxygen, silicon, and calcium, as well as trace of aluminium and chloride. Meanwhile, FTIR and XRD findings suggested that all sealers predominantly contained calcium silicate hydrate, calcium carbonate, and zirconium dioxide, while calcium aluminium silicate oxide was detected in 0-5% algin Bio-G. 5% algin Bio-G showed the highest mean push-out bond strength (p<0.05) with more favourable adhesion pattern, while iRoot SP showed the greatest sealer penetration (p<0.05). In addition, no significant association was noted between the dislodgement resistance and dentinal tubule penetration (p>0.05). In conclusion, the present novel Bio-G sealer demonstrated desirable particle size distribution and acceptable degree of purity. Algin-incorporated Bio-G showed favourable adhesive pattern with comparable dislodgement resistance and dentinal tubule penetration values to commercialised bioceramic-based sealers. 2023-06 Thesis http://eprints.usm.my/59823/ http://eprints.usm.my/59823/1/GALVIN%20SIM%20SIANG%20LIN%20-%20FINAL%20THESIS%20P-SGD000321%28R%29-E.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Pergigian
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine RK1-715 Dentistry
spellingShingle	R Medicine RK1-715 Dentistry Lin, Galvin Sim Siang Development of a novel bioceramic root canal sealer and its effect on the dislodgement resistance and dentinal tubule penetration
description	The present study aimed to fabricate and characterise novel algin-incorporated bioactive glass 58S calcium-silicate root canal sealer (Bio-G) and evaluate its effect on the dislodgement resistance and dentinal tubule penetration to root dentinal walls in comparison to commercially available bioceramic-based sealers (BioRoot RCS and iRoot SP). Three groups of bioactive glass (BG) 58S powders were synthesised using sol-gel method and characterised using SEM, HRTEM and FTIR for optimisation: BG-1 (no ammonia), BG-2 (3 ml of ammonia), and BG-3 (5 ml of ammonia). Optimised BG-3 was used to fabricate the powder form of experimental Bio-G sealer with the addition of calcium silicate, zirconia dioxide, calcium carbonate and alginic acid powder. Meanwhile, the liquid form composed of 5% calcium chloride solution. The experimental 0-5% algin Bio-G sealers were then compared with BioRoot RCS and iRoot SP. Standardised disc specimens of mixed sealer materials (n=5 per group) were prepared and placed in an incubator to allow setting before characterising under SEM, HRTEM, EDS, FTIR and XRD. Next, one-hundred-and-seventy-six mandibular premolars were randomly assigned (n=16 per group): control, gutta-percha + 0-5% algin Bio-G, gutta-percha + BioRoot RCS, and gutta-percha + iRoot SP groups, with the exclusion of the control group in adhesive pattern and dentinal tubule penetration tests. They were instrumented, obturated and placed in an incubator for 72 hours to allow sealer set. For the dentinal tubule penetration test, sealers were mixed with 0.1% of rhodamine B dye. Subsequently, teeth were cut into a 1-mm-thick cross-section at 5-mm and 10-mm levels from the root apex, respectively. Push-out bond strength, adhesive pattern and dentinal tubule penetration tests were performed. Experimental Bio-G sealer revealed irregular micro-sized particles with a higher content of oxygen, silicon, and calcium, as well as trace of aluminium and chloride. Meanwhile, FTIR and XRD findings suggested that all sealers predominantly contained calcium silicate hydrate, calcium carbonate, and zirconium dioxide, while calcium aluminium silicate oxide was detected in 0-5% algin Bio-G. 5% algin Bio-G showed the highest mean push-out bond strength (p<0.05) with more favourable adhesion pattern, while iRoot SP showed the greatest sealer penetration (p<0.05). In addition, no significant association was noted between the dislodgement resistance and dentinal tubule penetration (p>0.05). In conclusion, the present novel Bio-G sealer demonstrated desirable particle size distribution and acceptable degree of purity. Algin-incorporated Bio-G showed favourable adhesive pattern with comparable dislodgement resistance and dentinal tubule penetration values to commercialised bioceramic-based sealers.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Lin, Galvin Sim Siang
author_facet	Lin, Galvin Sim Siang
author_sort	Lin, Galvin Sim Siang
title	Development of a novel bioceramic root canal sealer and its effect on the dislodgement resistance and dentinal tubule penetration
title_short	Development of a novel bioceramic root canal sealer and its effect on the dislodgement resistance and dentinal tubule penetration
title_full	Development of a novel bioceramic root canal sealer and its effect on the dislodgement resistance and dentinal tubule penetration
title_fullStr	Development of a novel bioceramic root canal sealer and its effect on the dislodgement resistance and dentinal tubule penetration
title_full_unstemmed	Development of a novel bioceramic root canal sealer and its effect on the dislodgement resistance and dentinal tubule penetration
title_sort	development of a novel bioceramic root canal sealer and its effect on the dislodgement resistance and dentinal tubule penetration
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Pergigian
publishDate	2023
url	http://eprints.usm.my/59823/1/GALVIN%20SIM%20SIANG%20LIN%20-%20FINAL%20THESIS%20P-SGD000321%28R%29-E.pdf
_version_	1794024063525453824

Development of a novel bioceramic root canal sealer and its effect on the dislodgement resistance and dentinal tubule penetration

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