Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant

Bioflocculants are important to remove pollutants, especially organic particles and heavy metals from wastewater. Previous study showed that Aureispira sp. CCB-QB1 demonstrated cell aggregation when calcium ions are available. This suggested that these bacterial cells are capable to adsorb the wa...

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Main Author:	Rahman, Nur Hasyimah Abdul
Format:	Thesis
Language:	English
Published:	2022
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/59874/1/NUR%20HASYIMAH%20BINTI%20ABDUL%20RAHMAN%20-%20TESIS24.pdf
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id	my-usm-ep.59874
record_format	uketd_dc
spelling	my-usm-ep.598742024-02-14T04:42:33Z Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant 2022-05 Rahman, Nur Hasyimah Abdul QH1 Natural history (General - Including nature conservation, geographical distribution) Bioflocculants are important to remove pollutants, especially organic particles and heavy metals from wastewater. Previous study showed that Aureispira sp. CCB-QB1 demonstrated cell aggregation when calcium ions are available. This suggested that these bacterial cells are capable to adsorb the waste materials and have a great chance to be a novel bioflocculant. Therefore, in this research, the methods for cultivation of Aureispira sp. CCB-QB1 inside a bioreactor was studied. Then, the biosorption activity of the dead cells toward heavy metals (Fe3+ and Cu2+) was tested. The QB1 dead cells were prepared by treating the living cells using 0.5 % of formaldehyde. The dead cells were discovered to show robust flocculating activity toward kaolin (initial concentration 0.2 %) in the presence of 7 mM CaCl2. For cultivation inside flasks, 5 ml of the dead cell suspension showed overall of 96 % of flocculating activity after shaken using both 150 and 200 rpm agitation speeds. Besides, both tryptone and peptone were found to highly supported the growth of QB1 cells. The presence of peptone in the cell culture also helps in the aggregation of the QB1 cells. For cultivation inside a bioreactor, QB1 culture conditions were optimized using Response Surface Methodology (RSM). The optimized conditions were 3.39 % of sea salt concentration, 0.9 % of peptone concentration, and 29 h of incubation period. Additionally 2022-05 Thesis http://eprints.usm.my/59874/ http://eprints.usm.my/59874/1/NUR%20HASYIMAH%20BINTI%20ABDUL%20RAHMAN%20-%20TESIS24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Rahman, Nur Hasyimah Abdul Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant
description	Bioflocculants are important to remove pollutants, especially organic particles and heavy metals from wastewater. Previous study showed that Aureispira sp. CCB-QB1 demonstrated cell aggregation when calcium ions are available. This suggested that these bacterial cells are capable to adsorb the waste materials and have a great chance to be a novel bioflocculant. Therefore, in this research, the methods for cultivation of Aureispira sp. CCB-QB1 inside a bioreactor was studied. Then, the biosorption activity of the dead cells toward heavy metals (Fe3+ and Cu2+) was tested. The QB1 dead cells were prepared by treating the living cells using 0.5 % of formaldehyde. The dead cells were discovered to show robust flocculating activity toward kaolin (initial concentration 0.2 %) in the presence of 7 mM CaCl2. For cultivation inside flasks, 5 ml of the dead cell suspension showed overall of 96 % of flocculating activity after shaken using both 150 and 200 rpm agitation speeds. Besides, both tryptone and peptone were found to highly supported the growth of QB1 cells. The presence of peptone in the cell culture also helps in the aggregation of the QB1 cells. For cultivation inside a bioreactor, QB1 culture conditions were optimized using Response Surface Methodology (RSM). The optimized conditions were 3.39 % of sea salt concentration, 0.9 % of peptone concentration, and 29 h of incubation period. Additionally
format	Thesis
qualification_level	Master's degree
author	Rahman, Nur Hasyimah Abdul
author_facet	Rahman, Nur Hasyimah Abdul
author_sort	Rahman, Nur Hasyimah Abdul
title	Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant
title_short	Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant
title_full	Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant
title_fullStr	Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant
title_full_unstemmed	Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant
title_sort	biomass production of aureispira sp. ccb-qb1 and its application as a novel bioflocculant
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2022
url	http://eprints.usm.my/59874/1/NUR%20HASYIMAH%20BINTI%20ABDUL%20RAHMAN%20-%20TESIS24.pdf
_version_	1794024066758213632

Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant

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