Haemoglobin And Porphyrin Encapsulated Self-Assembled Polymers As Artificial Oxygen Carrier

Blood transfusion services have a lot of risks such as blood-associated risk, blood incompatibility and shortage of blood supply. To address this issue, researchers explored an artificial oxygen carrier through macromolecules by mimicking the structure of human haemoglobin. The aim of this study is...

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Main Author:	Othman, Mislia
Format:	Thesis
Language:	English
Published:	2023
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/59999/1/MISLIA%20BINTI%20OTHMAN%20-%20TESIS%20cut.pdf
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spelling	my-usm-ep.599992024-02-26T03:06:36Z Haemoglobin And Porphyrin Encapsulated Self-Assembled Polymers As Artificial Oxygen Carrier 2023-10 Othman, Mislia QH1 Natural history (General - Including nature conservation, geographical distribution) Blood transfusion services have a lot of risks such as blood-associated risk, blood incompatibility and shortage of blood supply. To address this issue, researchers explored an artificial oxygen carrier through macromolecules by mimicking the structure of human haemoglobin. The aim of this study is to develop haemoglobin and porphyrin encapsulated self-assembled polymers as artificial oxygen carrier. A porphyrin derivative, tetrakis-4-phenyl porphyrin (TPP) was first synthesised through the reaction of pyrrole and 4-benzaldehyde following the addition of iron, Fe(III) forming tetrakis-4-phenyl porphyrin Fe(III) (TPP Fe(III)). Methoxy-polyethylene glycol (mPEG45) block and poly (ɛ-caprolactone) (ɛ-CL) (mPEG-b-PCL) that formed diblock copolymer and para-nitrophenol core and glycidol that formed hyperbranched polyglycidol (HPG) was synthesised via ring-opening polymerisation (ROP). Extra cted Hb and synthesised TPP Fe(III) were then encapsulated in micelle system with a critical micelle concentration (CMC) at 1.0-2.0 mg/ml and hyperbranched polyglycidol prepared in equal molar to Hb and TPP Fe(III) concentration. The average encapsulation efficiency (EE%) of human Hb-micelle and TPP Fe(III)-micelle were 67 ± 5.39% and 78 ± 1.38% as compared to 68 ± 2.22% of bovine Hb-micelle. On the other hand, the EE% of human Hb-HPG and TPP Fe(III)-HPG were 58 ± 6.87% and 22 ± 7.12% as compared to 65 ± 9.11% of bovine Hb-HPG. 2023-10 Thesis http://eprints.usm.my/59999/ http://eprints.usm.my/59999/1/MISLIA%20BINTI%20OTHMAN%20-%20TESIS%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Othman, Mislia Haemoglobin And Porphyrin Encapsulated Self-Assembled Polymers As Artificial Oxygen Carrier
description	Blood transfusion services have a lot of risks such as blood-associated risk, blood incompatibility and shortage of blood supply. To address this issue, researchers explored an artificial oxygen carrier through macromolecules by mimicking the structure of human haemoglobin. The aim of this study is to develop haemoglobin and porphyrin encapsulated self-assembled polymers as artificial oxygen carrier. A porphyrin derivative, tetrakis-4-phenyl porphyrin (TPP) was first synthesised through the reaction of pyrrole and 4-benzaldehyde following the addition of iron, Fe(III) forming tetrakis-4-phenyl porphyrin Fe(III) (TPP Fe(III)). Methoxy-polyethylene glycol (mPEG45) block and poly (ɛ-caprolactone) (ɛ-CL) (mPEG-b-PCL) that formed diblock copolymer and para-nitrophenol core and glycidol that formed hyperbranched polyglycidol (HPG) was synthesised via ring-opening polymerisation (ROP). Extra cted Hb and synthesised TPP Fe(III) were then encapsulated in micelle system with a critical micelle concentration (CMC) at 1.0-2.0 mg/ml and hyperbranched polyglycidol prepared in equal molar to Hb and TPP Fe(III) concentration. The average encapsulation efficiency (EE%) of human Hb-micelle and TPP Fe(III)-micelle were 67 ± 5.39% and 78 ± 1.38% as compared to 68 ± 2.22% of bovine Hb-micelle. On the other hand, the EE% of human Hb-HPG and TPP Fe(III)-HPG were 58 ± 6.87% and 22 ± 7.12% as compared to 65 ± 9.11% of bovine Hb-HPG.
format	Thesis
qualification_level	Master's degree
author	Othman, Mislia
author_facet	Othman, Mislia
author_sort	Othman, Mislia
title	Haemoglobin And Porphyrin Encapsulated Self-Assembled Polymers As Artificial Oxygen Carrier
title_short	Haemoglobin And Porphyrin Encapsulated Self-Assembled Polymers As Artificial Oxygen Carrier
title_full	Haemoglobin And Porphyrin Encapsulated Self-Assembled Polymers As Artificial Oxygen Carrier
title_fullStr	Haemoglobin And Porphyrin Encapsulated Self-Assembled Polymers As Artificial Oxygen Carrier
title_full_unstemmed	Haemoglobin And Porphyrin Encapsulated Self-Assembled Polymers As Artificial Oxygen Carrier
title_sort	haemoglobin and porphyrin encapsulated self-assembled polymers as artificial oxygen carrier
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2023
url	http://eprints.usm.my/59999/1/MISLIA%20BINTI%20OTHMAN%20-%20TESIS%20cut.pdf
_version_	1794024074491461632

Haemoglobin And Porphyrin Encapsulated Self-Assembled Polymers As Artificial Oxygen Carrier

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