Genomic Expression Profile Of Human Papillomavirus 16 And 18 – Associated Pre-cancerous Lesions And Cancer Of The Cervix

Cervical cancer is one of the most common cancers in women and is caused by high-risk human papillomavirus (HPV) infection. The integration of HPV into host cervical epithelial cells causes genetic alterations and consequent changes in gene expression affecting downstream molecular pathways, leading...

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Main Author:	Balasubramaniam, Shandra Devi
Format:	Thesis
Language:	English
Published:	2022
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/60003/1/SHANDRA%20DEVI%20AP%20BALASUBRAMANIAM%20-%20TESIS24.pdf
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spelling	my-usm-ep.600032024-02-27T01:10:27Z Genomic Expression Profile Of Human Papillomavirus 16 And 18 – Associated Pre-cancerous Lesions And Cancer Of The Cervix 2022-06 Balasubramaniam, Shandra Devi R5-920 Medicine (General) Cervical cancer is one of the most common cancers in women and is caused by high-risk human papillomavirus (HPV) infection. The integration of HPV into host cervical epithelial cells causes genetic alterations and consequent changes in gene expression affecting downstream molecular pathways, leading to the development of cervical cancer. This study aimed to profile the genomic signatures involved in the pathogenesis of HPV 16 and 18 associated pre-cancerous lesions (cervical intraepithelial neoplasia, CIN) and squamous cell carcinoma (SCC) of the cervix, in comparison to the normal cervix. The differential mRNA expression profiles were determined, to evaluate the expression of up-regulated and down-regulated host genes and to evaluate the perturbed molecular pathways involved in cervical carcinogenesis. In this study, 29 formalin-fixed paraffin-embedded (FFPE) tissues including low-grade CIN (LGCIN), high-grade CIN (HGCIN), SCC, and normal cervix were screened for HPV 16 and HPV 18 using immunohistochemistry and qRT-PCR method. Of these, 9 HPV-positive (3 LGCIN, 3 HGCIN, 3 SCC) and 3 normal cervix (HPV-negative) tissue samples were microdissected to obtain regions of interest in the squamous epithelium before RNA extraction. Transcriptomic profiling was performed using the Affymetrix, GeneChip Human Transcriptome Array 2.0 (HTA 2.0) and the nCounter® PanCancer Pathway Array, Nanostring to identify differentially expressed genes (DEGs) and significantly associated pathways in each stage of cervical cancer development. 2022-06 Thesis http://eprints.usm.my/60003/ http://eprints.usm.my/60003/1/SHANDRA%20DEVI%20AP%20BALASUBRAMANIAM%20-%20TESIS24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Balasubramaniam, Shandra Devi Genomic Expression Profile Of Human Papillomavirus 16 And 18 – Associated Pre-cancerous Lesions And Cancer Of The Cervix
description	Cervical cancer is one of the most common cancers in women and is caused by high-risk human papillomavirus (HPV) infection. The integration of HPV into host cervical epithelial cells causes genetic alterations and consequent changes in gene expression affecting downstream molecular pathways, leading to the development of cervical cancer. This study aimed to profile the genomic signatures involved in the pathogenesis of HPV 16 and 18 associated pre-cancerous lesions (cervical intraepithelial neoplasia, CIN) and squamous cell carcinoma (SCC) of the cervix, in comparison to the normal cervix. The differential mRNA expression profiles were determined, to evaluate the expression of up-regulated and down-regulated host genes and to evaluate the perturbed molecular pathways involved in cervical carcinogenesis. In this study, 29 formalin-fixed paraffin-embedded (FFPE) tissues including low-grade CIN (LGCIN), high-grade CIN (HGCIN), SCC, and normal cervix were screened for HPV 16 and HPV 18 using immunohistochemistry and qRT-PCR method. Of these, 9 HPV-positive (3 LGCIN, 3 HGCIN, 3 SCC) and 3 normal cervix (HPV-negative) tissue samples were microdissected to obtain regions of interest in the squamous epithelium before RNA extraction. Transcriptomic profiling was performed using the Affymetrix, GeneChip Human Transcriptome Array 2.0 (HTA 2.0) and the nCounter® PanCancer Pathway Array, Nanostring to identify differentially expressed genes (DEGs) and significantly associated pathways in each stage of cervical cancer development.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Balasubramaniam, Shandra Devi
author_facet	Balasubramaniam, Shandra Devi
author_sort	Balasubramaniam, Shandra Devi
title	Genomic Expression Profile Of Human Papillomavirus 16 And 18 – Associated Pre-cancerous Lesions And Cancer Of The Cervix
title_short	Genomic Expression Profile Of Human Papillomavirus 16 And 18 – Associated Pre-cancerous Lesions And Cancer Of The Cervix
title_full	Genomic Expression Profile Of Human Papillomavirus 16 And 18 – Associated Pre-cancerous Lesions And Cancer Of The Cervix
title_fullStr	Genomic Expression Profile Of Human Papillomavirus 16 And 18 – Associated Pre-cancerous Lesions And Cancer Of The Cervix
title_full_unstemmed	Genomic Expression Profile Of Human Papillomavirus 16 And 18 – Associated Pre-cancerous Lesions And Cancer Of The Cervix
title_sort	genomic expression profile of human papillomavirus 16 and 18 – associated pre-cancerous lesions and cancer of the cervix
granting_institution	Universiti Sains Malaysia
granting_department	Institut Penyelidikan Perubatan Molekul
publishDate	2022
url	http://eprints.usm.my/60003/1/SHANDRA%20DEVI%20AP%20BALASUBRAMANIAM%20-%20TESIS24.pdf
_version_	1794024075477123072

Genomic Expression Profile Of Human Papillomavirus 16 And 18 – Associated Pre-cancerous Lesions And Cancer Of The Cervix

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