Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release

Sea cucumbers (Stichopus horrens) contain important bioactive compounds called sulfated polysaccharides (SP), which can be extracted by autolysis process utilizing its endogenous enzyme. However, this process is slow and not efficient. The aim of this study was to evaluate the mechanism of UV ass...

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Main Author:	Umam, Nada Itorul
Format:	Thesis
Language:	English
Published:	2023
Subjects:	TP368-456 Food processing and manufacture
Online Access:	http://eprints.usm.my/60013/1/NADA%20ITORUL%20UMAM%20-%20TESIS%27%20cut.pdf
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id	my-usm-ep.60013
record_format	uketd_dc
spelling	my-usm-ep.600132024-02-27T07:59:38Z Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release 2023-04 Umam, Nada Itorul TP368-456 Food processing and manufacture Sea cucumbers (Stichopus horrens) contain important bioactive compounds called sulfated polysaccharides (SP), which can be extracted by autolysis process utilizing its endogenous enzyme. However, this process is slow and not efficient. The aim of this study was to evaluate the mechanism of UV assisted autolysis in enhancing the release of SP from sea cucumber body wall. In phase I, the effects of UV dosage and pH on endogenous proteases activity, biochemical, and structural changes of SHBW during autolysis were evaluated. Frozen SHBW was homogenized with and without buffer (pH 6, 7, 8) and exposed to low (10,800 J/m2) and high (324,000 J/m2) UV dosage prior to autolysis at 50 °C for 4 and 8 hours. The endogenous protease activity and respective biochemical changes were evaluated from the soluble fraction while the insoluble fraction of SHBW was evaluated for secondary protein structural changes and thermal stability using Fourier-transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), and Thermogravimetry Analysis (TGA). Increase in UV dosage by 30x, increased proteolytic activity by 4.5-fold after 4 hours autolysis, resulting in increased soluble protein, hydroxyproline, and glycosaminoglycan (GAG) content by 5, 4, and 3 folds, respectively. Although sample without buffer showed similar endogenous protease activity with control sample, the total soluble protein, hydroxyproline, and GAG were higher by 4, 0.1, and 2.5 folds, respectively. 2023-04 Thesis http://eprints.usm.my/60013/ http://eprints.usm.my/60013/1/NADA%20ITORUL%20UMAM%20-%20TESIS%27%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Teknologi Industri
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	TP368-456 Food processing and manufacture
spellingShingle	TP368-456 Food processing and manufacture Umam, Nada Itorul Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release
description	Sea cucumbers (Stichopus horrens) contain important bioactive compounds called sulfated polysaccharides (SP), which can be extracted by autolysis process utilizing its endogenous enzyme. However, this process is slow and not efficient. The aim of this study was to evaluate the mechanism of UV assisted autolysis in enhancing the release of SP from sea cucumber body wall. In phase I, the effects of UV dosage and pH on endogenous proteases activity, biochemical, and structural changes of SHBW during autolysis were evaluated. Frozen SHBW was homogenized with and without buffer (pH 6, 7, 8) and exposed to low (10,800 J/m2) and high (324,000 J/m2) UV dosage prior to autolysis at 50 °C for 4 and 8 hours. The endogenous protease activity and respective biochemical changes were evaluated from the soluble fraction while the insoluble fraction of SHBW was evaluated for secondary protein structural changes and thermal stability using Fourier-transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), and Thermogravimetry Analysis (TGA). Increase in UV dosage by 30x, increased proteolytic activity by 4.5-fold after 4 hours autolysis, resulting in increased soluble protein, hydroxyproline, and glycosaminoglycan (GAG) content by 5, 4, and 3 folds, respectively. Although sample without buffer showed similar endogenous protease activity with control sample, the total soluble protein, hydroxyproline, and GAG were higher by 4, 0.1, and 2.5 folds, respectively.
format	Thesis
qualification_level	Master's degree
author	Umam, Nada Itorul
author_facet	Umam, Nada Itorul
author_sort	Umam, Nada Itorul
title	Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release
title_short	Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release
title_full	Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release
title_fullStr	Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release
title_full_unstemmed	Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release
title_sort	ultraviolet assisted autolysis of sea cucumber (stichopus horrens) body wall for the enhancement of sulfated polysaccharides release
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Teknologi Industri
publishDate	2023
url	http://eprints.usm.my/60013/1/NADA%20ITORUL%20UMAM%20-%20TESIS%27%20cut.pdf
_version_	1794024076221612032

Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release

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