Antidepressant Effect Of Andrographis Paniculata (Burm.F.) Wall. Ex Nees Methanolic Extract And Andrographolide On Chronic Unpredictable Stress (Cus) Zebrafish Model Via Metabolomics Approach

Depression affected at least 322 million people worldwide. Treatment for depression has been studied for a long time. However, the current antidepressants have many associated problems, such as delays in symptom resolution and relapse. Plants have been used as the main source of leads in drug discov...

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Main Author:	Aldurrah, Zenab
Format:	Thesis
Language:	English
Published:	2023
Subjects:	RS1-441 Pharmacy and materia medica
Online Access:	http://eprints.usm.my/60053/1/ZENAB%20ALDURRAH%20-%20TESIS%20cut.pdf
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id	my-usm-ep.60053
record_format	uketd_dc
spelling	my-usm-ep.600532024-03-05T02:49:54Z Antidepressant Effect Of Andrographis Paniculata (Burm.F.) Wall. Ex Nees Methanolic Extract And Andrographolide On Chronic Unpredictable Stress (Cus) Zebrafish Model Via Metabolomics Approach 2023-07 Aldurrah, Zenab RS1-441 Pharmacy and materia medica Depression affected at least 322 million people worldwide. Treatment for depression has been studied for a long time. However, the current antidepressants have many associated problems, such as delays in symptom resolution and relapse. Plants have been used as the main source of leads in drug discovery for decades. Andrographis paniculata (A. paniculata) is a widely used plant in Asian traditional medicine. This research investigates the effect of A. paniculata extract and its major compound, andrographolide on the chronic unpredictable stress (CUS) zebrafish model. A. paniculata methanol extract’s constituents was profiled using HPLC-ESI-MS/MS, and acute toxicity tests were analyzed before antidepressive evaluation. The behavioural study, i.e. open field test (OFT), social interaction test (SIT), and light dark test (LDT), were conducted to evaluate behavioural changes in the CUS group compared to the controls, A. paniculata (100 mg/L, immersion)-treated and fluoxetine (0.01 mg/L, immersion)-treated zebrafish. After the extract screening, the experiment was preceded by evaluating the antidepressive potential of andrographolide. Behavioural, cortisol and NMR-based metabolomics studies were evaluated in all groups. The behavioural study revealed a significant improvement in zebrafish locomotion after A. paniculata and andrographolide treatments. A significant reduction in cortisol levels (t-test, p=0.0003) were observed after andrographolide (25 and 50 mg/kg, i.p.) acute treatment. 2023-07 Thesis http://eprints.usm.my/60053/ http://eprints.usm.my/60053/1/ZENAB%20ALDURRAH%20-%20TESIS%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Farmasi
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RS1-441 Pharmacy and materia medica
spellingShingle	RS1-441 Pharmacy and materia medica Aldurrah, Zenab Antidepressant Effect Of Andrographis Paniculata (Burm.F.) Wall. Ex Nees Methanolic Extract And Andrographolide On Chronic Unpredictable Stress (Cus) Zebrafish Model Via Metabolomics Approach
description	Depression affected at least 322 million people worldwide. Treatment for depression has been studied for a long time. However, the current antidepressants have many associated problems, such as delays in symptom resolution and relapse. Plants have been used as the main source of leads in drug discovery for decades. Andrographis paniculata (A. paniculata) is a widely used plant in Asian traditional medicine. This research investigates the effect of A. paniculata extract and its major compound, andrographolide on the chronic unpredictable stress (CUS) zebrafish model. A. paniculata methanol extract’s constituents was profiled using HPLC-ESI-MS/MS, and acute toxicity tests were analyzed before antidepressive evaluation. The behavioural study, i.e. open field test (OFT), social interaction test (SIT), and light dark test (LDT), were conducted to evaluate behavioural changes in the CUS group compared to the controls, A. paniculata (100 mg/L, immersion)-treated and fluoxetine (0.01 mg/L, immersion)-treated zebrafish. After the extract screening, the experiment was preceded by evaluating the antidepressive potential of andrographolide. Behavioural, cortisol and NMR-based metabolomics studies were evaluated in all groups. The behavioural study revealed a significant improvement in zebrafish locomotion after A. paniculata and andrographolide treatments. A significant reduction in cortisol levels (t-test, p=0.0003) were observed after andrographolide (25 and 50 mg/kg, i.p.) acute treatment.
format	Thesis
qualification_level	Master's degree
author	Aldurrah, Zenab
author_facet	Aldurrah, Zenab
author_sort	Aldurrah, Zenab
title	Antidepressant Effect Of Andrographis Paniculata (Burm.F.) Wall. Ex Nees Methanolic Extract And Andrographolide On Chronic Unpredictable Stress (Cus) Zebrafish Model Via Metabolomics Approach
title_short	Antidepressant Effect Of Andrographis Paniculata (Burm.F.) Wall. Ex Nees Methanolic Extract And Andrographolide On Chronic Unpredictable Stress (Cus) Zebrafish Model Via Metabolomics Approach
title_full	Antidepressant Effect Of Andrographis Paniculata (Burm.F.) Wall. Ex Nees Methanolic Extract And Andrographolide On Chronic Unpredictable Stress (Cus) Zebrafish Model Via Metabolomics Approach
title_fullStr	Antidepressant Effect Of Andrographis Paniculata (Burm.F.) Wall. Ex Nees Methanolic Extract And Andrographolide On Chronic Unpredictable Stress (Cus) Zebrafish Model Via Metabolomics Approach
title_full_unstemmed	Antidepressant Effect Of Andrographis Paniculata (Burm.F.) Wall. Ex Nees Methanolic Extract And Andrographolide On Chronic Unpredictable Stress (Cus) Zebrafish Model Via Metabolomics Approach
title_sort	antidepressant effect of andrographis paniculata (burm.f.) wall. ex nees methanolic extract and andrographolide on chronic unpredictable stress (cus) zebrafish model via metabolomics approach
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Farmasi
publishDate	2023
url	http://eprints.usm.my/60053/1/ZENAB%20ALDURRAH%20-%20TESIS%20cut.pdf
_version_	1794024083900334080

Antidepressant Effect Of Andrographis Paniculata (Burm.F.) Wall. Ex Nees Methanolic Extract And Andrographolide On Chronic Unpredictable Stress (Cus) Zebrafish Model Via Metabolomics Approach

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