Characterisation, Pathogenicity, And Mycotoxin Analysis Of Fungal Endophytes Of Corn (Zea Mays L.) In Peninsular Malaysia
Endophytes are microbes that infect internal tissues of host plants for all or part of their life cycles, without causing any visible symptoms of disease. Depending on a number of factors such as the developmental stage of both host plant and fungus, environmental factors, and host defence respon...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2022
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| Subjects: | |
| Online Access: | http://eprints.usm.my/60076/1/TERNA%20TERSOO%20PAUL%20-%20TESIS24.pdf |
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| Summary: | Endophytes are microbes that infect internal tissues of host plants for all or part
of their life cycles, without causing any visible symptoms of disease. Depending on a
number of factors such as the developmental stage of both host plant and fungus,
environmental factors, and host defence responses, fungal endophytes indwelling
tissues of host plants may become latent plant pathogens. In Peninsular Malaysia,
works on endophytic fungi carried out so far have focused mainly on their ecological
and functional roles in enhancing plant growth and other survival attributes, while
much remains unknown regarding the disease initiation, and mycotoxin-producing
potentials of fungal endophytes especially of corn and other cereal crops. Thus, the
present study was carried out to identify, evaluate the pathogenicity, and investigate
the mycotoxin-producing potentials of endophytic fungi resident in corn plants grown
on different fields in Peninsular Malaysia. Using a combination of morphological and
molecular techniques, endophytic fungi recovered from tissues of corn plants were
identified in seven genera, namely Fusarium, Penicillium, Aspergillus,
Aureobasidium, Cladosporium, Epicoccum, and Curvularia. Based on BLAST search
and phylogenetic analysis of combined β-tubulin and TEF-1α sequences, endophytic
Fusarium species isolated from corn plants were molecularly identified as F.
pseudocircinatum, F. verticillioides, F. andiyazi, F. sacchari, F. mangiferae, F.
fujikuroi, F. proliferatum, and F. incarnatum. Endophytic Penicillium species were
identified as P. oxalicum, P. polonicum, and P. citrinum, based on ITS region and β- tubulin gene. Combined sequences of ITS region, β-tubulin, and Calmodulin genes
were used to identify endophytic Aspergillus species as A. flavus, and A. tubingensis,
ITS region and β-tubulin gene for the identification of A. pullulans, ITS region and
ACT gene for identification of C. tenuissimum, while combined ITS region and LSU
gene were used for identification of E. sorghinum and C. lunata. |
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