Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens

Toxocariasis is a neglected zoonotic parasitic disease caused by intestinal parasitic roundworms of dogs (Toxocara canis) and cats (Toxocara cati). Human toxocariasis has a global distribution and affects mostly people who are socioeconomically deprived. Clinicians face problems in diagnosing human...

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Main Author:	Baharudeen, Zamrina Mohd
Format:	Thesis
Language:	English
Published:	2023
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/60086/1/ZAMRINA%20BINTI%20MOHD%20BAHARUDEEN%20-%20TESIS%20cut.pdf
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spelling	my-usm-ep.600862024-03-08T08:06:09Z Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens 2023-07 Baharudeen, Zamrina Mohd R5-920 Medicine (General) Toxocariasis is a neglected zoonotic parasitic disease caused by intestinal parasitic roundworms of dogs (Toxocara canis) and cats (Toxocara cati). Human toxocariasis has a global distribution and affects mostly people who are socioeconomically deprived. Clinicians face problems in diagnosing human toxocariasis since the signs and symptoms are non-specific and similar to other helminthic infections. Most serodiagnostic methods for toxocariasis detection rely on IgG antibody-based assays using native antigen of T. canis, however the drawback is the lack of high diagnostic specificity due to cross-reactivity with antibodies to other helminths. Additionally, IgG is a long-lasting antibody, thus IgG assays may also detect past (cured) infections. Thus, there is a need to improve the serodiagnosis of toxocariasis and one good way is by developing an antigen detection assay. To develop such an assay, recombinant monoclonal antibodies (rmAbs) to Toxocara excretory-secretory (TES) antigens can be used as the capture reagent. In this study, two recombinant proteins namely T. canis (rTES-26) and T. cati (rTES-120 cati), were expressed and purified. The isolation of mAbs to the recombinant proteins was performed via biopanning using previously produced helminth phage display immune library. Five mAbs against rTES-26 antigen were successfully isolated. However, mAbs against rTES-120 cati antigen showed incomplete scFv sequences; hence, further analysis could not be performed. Henceforth, only the mAbs of the rTES-26 antigen were characterized based on gene family, length of sequence and amino acid distribution. 2023-07 Thesis http://eprints.usm.my/60086/ http://eprints.usm.my/60086/1/ZAMRINA%20BINTI%20MOHD%20BAHARUDEEN%20-%20TESIS%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Baharudeen, Zamrina Mohd Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens
description	Toxocariasis is a neglected zoonotic parasitic disease caused by intestinal parasitic roundworms of dogs (Toxocara canis) and cats (Toxocara cati). Human toxocariasis has a global distribution and affects mostly people who are socioeconomically deprived. Clinicians face problems in diagnosing human toxocariasis since the signs and symptoms are non-specific and similar to other helminthic infections. Most serodiagnostic methods for toxocariasis detection rely on IgG antibody-based assays using native antigen of T. canis, however the drawback is the lack of high diagnostic specificity due to cross-reactivity with antibodies to other helminths. Additionally, IgG is a long-lasting antibody, thus IgG assays may also detect past (cured) infections. Thus, there is a need to improve the serodiagnosis of toxocariasis and one good way is by developing an antigen detection assay. To develop such an assay, recombinant monoclonal antibodies (rmAbs) to Toxocara excretory-secretory (TES) antigens can be used as the capture reagent. In this study, two recombinant proteins namely T. canis (rTES-26) and T. cati (rTES-120 cati), were expressed and purified. The isolation of mAbs to the recombinant proteins was performed via biopanning using previously produced helminth phage display immune library. Five mAbs against rTES-26 antigen were successfully isolated. However, mAbs against rTES-120 cati antigen showed incomplete scFv sequences; hence, further analysis could not be performed. Henceforth, only the mAbs of the rTES-26 antigen were characterized based on gene family, length of sequence and amino acid distribution.
format	Thesis
qualification_level	Master's degree
author	Baharudeen, Zamrina Mohd
author_facet	Baharudeen, Zamrina Mohd
author_sort	Baharudeen, Zamrina Mohd
title	Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens
title_short	Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens
title_full	Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens
title_fullStr	Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens
title_full_unstemmed	Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens
title_sort	production and characterization of recombinant monoclonal antibodies against toxocara antigens
granting_institution	Universiti Sains Malaysia
granting_department	Institut Penyelidikan Perubatan Molekul
publishDate	2023
url	http://eprints.usm.my/60086/1/ZAMRINA%20BINTI%20MOHD%20BAHARUDEEN%20-%20TESIS%20cut.pdf
_version_	1794024088852758528

Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens

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