Characterisation Of Fungi Associated With Diseased Cocoa (Theobroma Cacao) In West Coast Of Peninsular Malaysia

Cocoa (Theobroma cacao) is an evergreen tree belonging to the Malvaceae family. The cacao plant has contributed significantly to the country's economy and has also become a source of income for smallholder farmers. However, fungal diseases are one of the major problems in cocoa plantations, whe...

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Main Author:	Rahim, Huda Shakirah Abd
Format:	Thesis
Language:	English
Published:	2023
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/60106/1/HUDA%20SHAKIRAH%20BINTI%20ABD%20RAHIM%20-%20TESIS%20cut.pdf
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id	my-usm-ep.60106
record_format	uketd_dc
spelling	my-usm-ep.601062024-03-11T07:18:56Z Characterisation Of Fungi Associated With Diseased Cocoa (Theobroma Cacao) In West Coast Of Peninsular Malaysia 2023-07 Rahim, Huda Shakirah Abd QH1 Natural history (General - Including nature conservation, geographical distribution) Cocoa (Theobroma cacao) is an evergreen tree belonging to the Malvaceae family. The cacao plant has contributed significantly to the country's economy and has also become a source of income for smallholder farmers. However, fungal diseases are one of the major problems in cocoa plantations, where the spread of uncontrollable infections can lead to significant yield losses. The objective of this study was to identify and characterise the fungal isolates associated with diseased T. cacao in Malaysia through morphological, molecular, and pathogenicity analyses. Diseased samples of leaves, stems, and pods of T. cacao were collected from different plantations in Malaysia. Morphological and molecular analyses were carried out to identify the fungal isolates. Phylogenetic analysis was carried out to accurately identify and characterise the fungal isolates using various genes such as internal transcribed spacer (ITS), translational elongation factor 1-alpha (tef1-α), β-tubulin (tub2), glyceraldehyde-3-phosphate dehydrogenase (gapdh), and RNA polymerase subunit II (rpb2). To assess the pathogenic capabilities of the fungal isolates, pathogenicity tests were carried out on healthy leaves, stems, and pods of T. cacao using mycelial plugs with wound treatment. A total of 116 fungal isolates were isolated from diseased leaves, stems, and pods of T. 2023-07 Thesis http://eprints.usm.my/60106/ http://eprints.usm.my/60106/1/HUDA%20SHAKIRAH%20BINTI%20ABD%20RAHIM%20-%20TESIS%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Rahim, Huda Shakirah Abd Characterisation Of Fungi Associated With Diseased Cocoa (Theobroma Cacao) In West Coast Of Peninsular Malaysia
description	Cocoa (Theobroma cacao) is an evergreen tree belonging to the Malvaceae family. The cacao plant has contributed significantly to the country's economy and has also become a source of income for smallholder farmers. However, fungal diseases are one of the major problems in cocoa plantations, where the spread of uncontrollable infections can lead to significant yield losses. The objective of this study was to identify and characterise the fungal isolates associated with diseased T. cacao in Malaysia through morphological, molecular, and pathogenicity analyses. Diseased samples of leaves, stems, and pods of T. cacao were collected from different plantations in Malaysia. Morphological and molecular analyses were carried out to identify the fungal isolates. Phylogenetic analysis was carried out to accurately identify and characterise the fungal isolates using various genes such as internal transcribed spacer (ITS), translational elongation factor 1-alpha (tef1-α), β-tubulin (tub2), glyceraldehyde-3-phosphate dehydrogenase (gapdh), and RNA polymerase subunit II (rpb2). To assess the pathogenic capabilities of the fungal isolates, pathogenicity tests were carried out on healthy leaves, stems, and pods of T. cacao using mycelial plugs with wound treatment. A total of 116 fungal isolates were isolated from diseased leaves, stems, and pods of T.
format	Thesis
qualification_level	Master's degree
author	Rahim, Huda Shakirah Abd
author_facet	Rahim, Huda Shakirah Abd
author_sort	Rahim, Huda Shakirah Abd
title	Characterisation Of Fungi Associated With Diseased Cocoa (Theobroma Cacao) In West Coast Of Peninsular Malaysia
title_short	Characterisation Of Fungi Associated With Diseased Cocoa (Theobroma Cacao) In West Coast Of Peninsular Malaysia
title_full	Characterisation Of Fungi Associated With Diseased Cocoa (Theobroma Cacao) In West Coast Of Peninsular Malaysia
title_fullStr	Characterisation Of Fungi Associated With Diseased Cocoa (Theobroma Cacao) In West Coast Of Peninsular Malaysia
title_full_unstemmed	Characterisation Of Fungi Associated With Diseased Cocoa (Theobroma Cacao) In West Coast Of Peninsular Malaysia
title_sort	characterisation of fungi associated with diseased cocoa (theobroma cacao) in west coast of peninsular malaysia
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2023
url	http://eprints.usm.my/60106/1/HUDA%20SHAKIRAH%20BINTI%20ABD%20RAHIM%20-%20TESIS%20cut.pdf
_version_	1794024091824422912

Characterisation Of Fungi Associated With Diseased Cocoa (Theobroma Cacao) In West Coast Of Peninsular Malaysia

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