Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-3- Hydroxyvalerate) From Cupriavidus Malaysiensis Usmaa1020 Using Glycerin Pitch As Main Carbon Source

Plastics are used in almost every part of modern life, from making aeroplanes, cars, and buildings to making clothes, shoes, packaging for food and drinks, and medical devices. As a result, plastic waste is gathered globally, worsening the effects on the ecosystem. Synthetic plastics/conventional pl...

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Main Author:	Rozina, Rozina
Format:	Thesis
Language:	English
Published:	2022
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/60124/1/24%20Pages%20from%20ROZINA.pdf
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id	my-usm-ep.60124
record_format	uketd_dc
spelling	my-usm-ep.601242024-03-12T01:47:25Z Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-3- Hydroxyvalerate) From Cupriavidus Malaysiensis Usmaa1020 Using Glycerin Pitch As Main Carbon Source 2022-11 Rozina, Rozina QH1-278.5 Natural history (General) Plastics are used in almost every part of modern life, from making aeroplanes, cars, and buildings to making clothes, shoes, packaging for food and drinks, and medical devices. As a result, plastic waste is gathered globally, worsening the effects on the ecosystem. Synthetic plastics/conventional plastics takes many years to disintegrate in nature, and incineration produces toxic substances and raises CO2 levels in the atmosphere, causing issues like global warming. As a result, alternatives to petrol-based polymers are being sought. Polyhydroxyalkanoates (PHAs) have been proposed as a feasible substitute for petrochemical-based polymers because of their biodegradable nature. In the present study, Cupriavidus malaysiensis USMAA1020, a Gram-negative bacterium was able to produce biodegradable polymer P(3HB-co- 3HV) by utilizing glycerin pitch which is a by-product of the biodiesel synthesis obtained during the transesterification as low-cost feedstock. The aim of the research was to screen various concentrations of glycerin pitch (5 g/L, 10 g/L, 15 g/L, 20 g/L, 25 g/L), 1-pentanol (0.03 wt% C, 0.06 wt% C, 0.09 wt% C, 0.12 wt% C), ammonium sulfate (0.8 g/L, 1.1 g/L, 1.4 g/L, 1.7 g/L, 2.0 g/L), and different precursors nonaoic acid, valeric acid, propionic acid, with respect to 1-pentanol at concentration of 0.06 wt%C. To enhance the production of PHA, oleic acid concentrations was used as a secondary carbon source. 2022-11 Thesis http://eprints.usm.my/60124/ http://eprints.usm.my/60124/1/24%20Pages%20from%20ROZINA.pdf application/pdf en public masters Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Rozina, Rozina Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-3- Hydroxyvalerate) From Cupriavidus Malaysiensis Usmaa1020 Using Glycerin Pitch As Main Carbon Source
description	Plastics are used in almost every part of modern life, from making aeroplanes, cars, and buildings to making clothes, shoes, packaging for food and drinks, and medical devices. As a result, plastic waste is gathered globally, worsening the effects on the ecosystem. Synthetic plastics/conventional plastics takes many years to disintegrate in nature, and incineration produces toxic substances and raises CO2 levels in the atmosphere, causing issues like global warming. As a result, alternatives to petrol-based polymers are being sought. Polyhydroxyalkanoates (PHAs) have been proposed as a feasible substitute for petrochemical-based polymers because of their biodegradable nature. In the present study, Cupriavidus malaysiensis USMAA1020, a Gram-negative bacterium was able to produce biodegradable polymer P(3HB-co- 3HV) by utilizing glycerin pitch which is a by-product of the biodiesel synthesis obtained during the transesterification as low-cost feedstock. The aim of the research was to screen various concentrations of glycerin pitch (5 g/L, 10 g/L, 15 g/L, 20 g/L, 25 g/L), 1-pentanol (0.03 wt% C, 0.06 wt% C, 0.09 wt% C, 0.12 wt% C), ammonium sulfate (0.8 g/L, 1.1 g/L, 1.4 g/L, 1.7 g/L, 2.0 g/L), and different precursors nonaoic acid, valeric acid, propionic acid, with respect to 1-pentanol at concentration of 0.06 wt%C. To enhance the production of PHA, oleic acid concentrations was used as a secondary carbon source.
format	Thesis
qualification_level	Master's degree
author	Rozina, Rozina
author_facet	Rozina, Rozina
author_sort	Rozina, Rozina
title	Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-3- Hydroxyvalerate) From Cupriavidus Malaysiensis Usmaa1020 Using Glycerin Pitch As Main Carbon Source
title_short	Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-3- Hydroxyvalerate) From Cupriavidus Malaysiensis Usmaa1020 Using Glycerin Pitch As Main Carbon Source
title_full	Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-3- Hydroxyvalerate) From Cupriavidus Malaysiensis Usmaa1020 Using Glycerin Pitch As Main Carbon Source
title_fullStr	Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-3- Hydroxyvalerate) From Cupriavidus Malaysiensis Usmaa1020 Using Glycerin Pitch As Main Carbon Source
title_full_unstemmed	Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-3- Hydroxyvalerate) From Cupriavidus Malaysiensis Usmaa1020 Using Glycerin Pitch As Main Carbon Source
title_sort	biosynthesis and characterization of poly(3-hydroxybutyrate-co-3- hydroxyvalerate) from cupriavidus malaysiensis usmaa1020 using glycerin pitch as main carbon source
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2022
url	http://eprints.usm.my/60124/1/24%20Pages%20from%20ROZINA.pdf
_version_	1794024093038673920

Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-3- Hydroxyvalerate) From Cupriavidus Malaysiensis Usmaa1020 Using Glycerin Pitch As Main Carbon Source

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