Diversity Of Bacteria In Breeding Water And Midgut Of Aedes Albopictus, And Determination Of Its Paratransgenic Candidates And Ovipositional Attractant

Researchers have started to focus on vector management and implementation of biological control to replace the hazardous effect of excessive insecticide usage. This study was conducted to provide fundamental knowledge of bacteria diversity in breeding water and midgut of Aedes albopictus. The ide...

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主要作者: Mohd Fadzlah, Fadhlina Hazwani
格式: Thesis
语言:English
出版: 2023
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spelling my-usm-ep.601592024-03-13T01:43:22Z Diversity Of Bacteria In Breeding Water And Midgut Of Aedes Albopictus, And Determination Of Its Paratransgenic Candidates And Ovipositional Attractant 2023-06 Mohd Fadzlah, Fadhlina Hazwani QH1-278.5 Natural history (General) Researchers have started to focus on vector management and implementation of biological control to replace the hazardous effect of excessive insecticide usage. This study was conducted to provide fundamental knowledge of bacteria diversity in breeding water and midgut of Aedes albopictus. The identification of these bacteria is important for its roles as paratransgenesis and oviposition attractant. The culture-dependent and culture-independent methods were used for the comparative assessment of bacteria from artificial containers with the presence and absence of larvae. Interestingly, the culture-dependant method showed a higher bacterial diversity in breeding water with the presence of larvae but higher operational taxonomic units (OTUs) in the absence of larvae by culture-independent method. These results highlighted the importance of a type of bacterium that is responsible for the attraction or deterrent effects towards mosquitoes. Higher microbiota diversity present in an adult mosquito’s midgut compared to larval stage concluded that both environmental and vertical inheritance are the microbiota sources in the midgut. The consistent presence of Bacillus cereus and Proteus mirabilis in breeding water and both stages of Ae. albopictus mosquito’s midgut served as potential early candidates for the paratransgenesis. The paratransgenic candidates tested for B. cereus and P. mirabilis showed that the B. cereus was beneficial and more suitable as paratransgenic candidate. 2023-06 Thesis http://eprints.usm.my/60159/ http://eprints.usm.my/60159/1/24%20Pages%20from%20FADHLINA%20HAZWANI%20BINTI%20MOHD%20FADZLAH.pdf application/pdf en public phd doctoral Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kajihayat
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic QH1-278.5 Natural history (General)
spellingShingle QH1-278.5 Natural history (General)
Mohd Fadzlah, Fadhlina Hazwani
Diversity Of Bacteria In Breeding Water And Midgut Of Aedes Albopictus, And Determination Of Its Paratransgenic Candidates And Ovipositional Attractant
description Researchers have started to focus on vector management and implementation of biological control to replace the hazardous effect of excessive insecticide usage. This study was conducted to provide fundamental knowledge of bacteria diversity in breeding water and midgut of Aedes albopictus. The identification of these bacteria is important for its roles as paratransgenesis and oviposition attractant. The culture-dependent and culture-independent methods were used for the comparative assessment of bacteria from artificial containers with the presence and absence of larvae. Interestingly, the culture-dependant method showed a higher bacterial diversity in breeding water with the presence of larvae but higher operational taxonomic units (OTUs) in the absence of larvae by culture-independent method. These results highlighted the importance of a type of bacterium that is responsible for the attraction or deterrent effects towards mosquitoes. Higher microbiota diversity present in an adult mosquito’s midgut compared to larval stage concluded that both environmental and vertical inheritance are the microbiota sources in the midgut. The consistent presence of Bacillus cereus and Proteus mirabilis in breeding water and both stages of Ae. albopictus mosquito’s midgut served as potential early candidates for the paratransgenesis. The paratransgenic candidates tested for B. cereus and P. mirabilis showed that the B. cereus was beneficial and more suitable as paratransgenic candidate.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Mohd Fadzlah, Fadhlina Hazwani
author_facet Mohd Fadzlah, Fadhlina Hazwani
author_sort Mohd Fadzlah, Fadhlina Hazwani
title Diversity Of Bacteria In Breeding Water And Midgut Of Aedes Albopictus, And Determination Of Its Paratransgenic Candidates And Ovipositional Attractant
title_short Diversity Of Bacteria In Breeding Water And Midgut Of Aedes Albopictus, And Determination Of Its Paratransgenic Candidates And Ovipositional Attractant
title_full Diversity Of Bacteria In Breeding Water And Midgut Of Aedes Albopictus, And Determination Of Its Paratransgenic Candidates And Ovipositional Attractant
title_fullStr Diversity Of Bacteria In Breeding Water And Midgut Of Aedes Albopictus, And Determination Of Its Paratransgenic Candidates And Ovipositional Attractant
title_full_unstemmed Diversity Of Bacteria In Breeding Water And Midgut Of Aedes Albopictus, And Determination Of Its Paratransgenic Candidates And Ovipositional Attractant
title_sort diversity of bacteria in breeding water and midgut of aedes albopictus, and determination of its paratransgenic candidates and ovipositional attractant
granting_institution Perpustakaan Hamzah Sendut
granting_department Pusat Pengajian Sains Kajihayat
publishDate 2023
url http://eprints.usm.my/60159/1/24%20Pages%20from%20FADHLINA%20HAZWANI%20BINTI%20MOHD%20FADZLAH.pdf
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