Diversity Of Bacteria In Breeding Water And Midgut Of Aedes Albopictus, And Determination Of Its Paratransgenic Candidates And Ovipositional Attractant
Researchers have started to focus on vector management and implementation of biological control to replace the hazardous effect of excessive insecticide usage. This study was conducted to provide fundamental knowledge of bacteria diversity in breeding water and midgut of Aedes albopictus. The ide...
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my-usm-ep.601592024-03-13T01:43:22Z Diversity Of Bacteria In Breeding Water And Midgut Of Aedes Albopictus, And Determination Of Its Paratransgenic Candidates And Ovipositional Attractant 2023-06 Mohd Fadzlah, Fadhlina Hazwani QH1-278.5 Natural history (General) Researchers have started to focus on vector management and implementation of biological control to replace the hazardous effect of excessive insecticide usage. This study was conducted to provide fundamental knowledge of bacteria diversity in breeding water and midgut of Aedes albopictus. The identification of these bacteria is important for its roles as paratransgenesis and oviposition attractant. The culture-dependent and culture-independent methods were used for the comparative assessment of bacteria from artificial containers with the presence and absence of larvae. Interestingly, the culture-dependant method showed a higher bacterial diversity in breeding water with the presence of larvae but higher operational taxonomic units (OTUs) in the absence of larvae by culture-independent method. These results highlighted the importance of a type of bacterium that is responsible for the attraction or deterrent effects towards mosquitoes. Higher microbiota diversity present in an adult mosquito’s midgut compared to larval stage concluded that both environmental and vertical inheritance are the microbiota sources in the midgut. The consistent presence of Bacillus cereus and Proteus mirabilis in breeding water and both stages of Ae. albopictus mosquito’s midgut served as potential early candidates for the paratransgenesis. The paratransgenic candidates tested for B. cereus and P. mirabilis showed that the B. cereus was beneficial and more suitable as paratransgenic candidate. 2023-06 Thesis http://eprints.usm.my/60159/ http://eprints.usm.my/60159/1/24%20Pages%20from%20FADHLINA%20HAZWANI%20BINTI%20MOHD%20FADZLAH.pdf application/pdf en public phd doctoral Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kajihayat |
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QH1-278.5 Natural history (General) Mohd Fadzlah, Fadhlina Hazwani Diversity Of Bacteria In Breeding Water And Midgut Of Aedes Albopictus, And Determination Of Its Paratransgenic Candidates And Ovipositional Attractant |
description |
Researchers have started to focus on vector management and implementation of
biological control to replace the hazardous effect of excessive insecticide usage. This study
was conducted to provide fundamental knowledge of bacteria diversity in breeding water and
midgut of Aedes albopictus. The identification of these bacteria is important for its roles as
paratransgenesis and oviposition attractant. The culture-dependent and culture-independent
methods were used for the comparative assessment of bacteria from artificial containers with
the presence and absence of larvae. Interestingly, the culture-dependant method showed a
higher bacterial diversity in breeding water with the presence of larvae but higher operational
taxonomic units (OTUs) in the absence of larvae by culture-independent method. These results
highlighted the importance of a type of bacterium that is responsible for the attraction or
deterrent effects towards mosquitoes. Higher microbiota diversity present in an adult
mosquito’s midgut compared to larval stage concluded that both environmental and vertical
inheritance are the microbiota sources in the midgut. The consistent presence of Bacillus cereus
and Proteus mirabilis in breeding water and both stages of Ae. albopictus mosquito’s midgut
served as potential early candidates for the paratransgenesis. The paratransgenic candidates
tested for B. cereus and P. mirabilis showed that the B. cereus was beneficial and more suitable
as paratransgenic candidate. |
format |
Thesis |
qualification_name |
Doctor of Philosophy (PhD.) |
qualification_level |
Doctorate |
author |
Mohd Fadzlah, Fadhlina Hazwani |
author_facet |
Mohd Fadzlah, Fadhlina Hazwani |
author_sort |
Mohd Fadzlah, Fadhlina Hazwani |
title |
Diversity Of Bacteria In Breeding Water And Midgut Of Aedes Albopictus, And Determination Of Its Paratransgenic Candidates And Ovipositional Attractant |
title_short |
Diversity Of Bacteria In Breeding Water And Midgut Of Aedes Albopictus, And Determination Of Its Paratransgenic Candidates And Ovipositional Attractant |
title_full |
Diversity Of Bacteria In Breeding Water And Midgut Of Aedes Albopictus, And Determination Of Its Paratransgenic Candidates And Ovipositional Attractant |
title_fullStr |
Diversity Of Bacteria In Breeding Water And Midgut Of Aedes Albopictus, And Determination Of Its Paratransgenic Candidates And Ovipositional Attractant |
title_full_unstemmed |
Diversity Of Bacteria In Breeding Water And Midgut Of Aedes Albopictus, And Determination Of Its Paratransgenic Candidates And Ovipositional Attractant |
title_sort |
diversity of bacteria in breeding water and midgut of aedes albopictus, and determination of its paratransgenic candidates and ovipositional attractant |
granting_institution |
Perpustakaan Hamzah Sendut |
granting_department |
Pusat Pengajian Sains Kajihayat |
publishDate |
2023 |
url |
http://eprints.usm.my/60159/1/24%20Pages%20from%20FADHLINA%20HAZWANI%20BINTI%20MOHD%20FADZLAH.pdf |
_version_ |
1794024098524823552 |