Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9

Andrographolide is a labdane diterpenoid isolated from the plant Andrographis paniculata. This substance has numerous medicinal uses, notably anticancer effects. A previous study has revealed that andrographolide inhibits the growth of lung, brain, colon, and breast cancer cells. Due to a lack of re...

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Main Author:	Manimaran, Janany
Format:	Thesis
Language:	English
Published:	2023
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/60182/1/24%20Pages%20from%20JANANY%20AP%20MANIMARAN.pdf
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id	my-usm-ep.60182
record_format	uketd_dc
spelling	my-usm-ep.601822024-03-14T03:03:51Z Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9 2023-04 Manimaran, Janany R5-920 Medicine (General) Andrographolide is a labdane diterpenoid isolated from the plant Andrographis paniculata. This substance has numerous medicinal uses, notably anticancer effects. A previous study has revealed that andrographolide inhibits the growth of lung, brain, colon, and breast cancer cells. Due to a lack of research, the knowledge of andrographolide's anti-cancer effects on prostate cancer cells is relatively poor. In the current study, andrographolide was assessed on PC-3 cells, an aggressive androgen-independent prostate cancer cell line. Cytotoxicity analysis is vital in drug discovery research for assessing the biocompatibility of the drug being used on cancer cells. This work used the WST-1 assay to determine the cell survival of PC-3 cancer cells and Hs27 normal cells exposed to varying doses of andrographolide (0-200 μM). The results indicate that andrographolide dose-dependently suppresses the viability of PC-3 cells but not Hs27 cells. The NCI considers the LC50 value of 26.42 μM (after 48 hours of incubation) is acceptable. For the first time in this study, three different concentrations of andrographolide were used: control, half LC50, and LC50 (0, 13.21, and 26.42 μM) in all subsequent analyses. Metastasis is essential for the disease to progress; hence, the scratch assay and the transwell invasion assay were used to test andrographolide on PC-3 cells. 2023-04 Thesis http://eprints.usm.my/60182/ http://eprints.usm.my/60182/1/24%20Pages%20from%20JANANY%20AP%20MANIMARAN.pdf application/pdf en public masters Perpustakaan Hamzah Sendut Institut Penyelidikan Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Manimaran, Janany Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9
description	Andrographolide is a labdane diterpenoid isolated from the plant Andrographis paniculata. This substance has numerous medicinal uses, notably anticancer effects. A previous study has revealed that andrographolide inhibits the growth of lung, brain, colon, and breast cancer cells. Due to a lack of research, the knowledge of andrographolide's anti-cancer effects on prostate cancer cells is relatively poor. In the current study, andrographolide was assessed on PC-3 cells, an aggressive androgen-independent prostate cancer cell line. Cytotoxicity analysis is vital in drug discovery research for assessing the biocompatibility of the drug being used on cancer cells. This work used the WST-1 assay to determine the cell survival of PC-3 cancer cells and Hs27 normal cells exposed to varying doses of andrographolide (0-200 μM). The results indicate that andrographolide dose-dependently suppresses the viability of PC-3 cells but not Hs27 cells. The NCI considers the LC50 value of 26.42 μM (after 48 hours of incubation) is acceptable. For the first time in this study, three different concentrations of andrographolide were used: control, half LC50, and LC50 (0, 13.21, and 26.42 μM) in all subsequent analyses. Metastasis is essential for the disease to progress; hence, the scratch assay and the transwell invasion assay were used to test andrographolide on PC-3 cells.
format	Thesis
qualification_level	Master's degree
author	Manimaran, Janany
author_facet	Manimaran, Janany
author_sort	Manimaran, Janany
title	Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9
title_short	Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9
title_full	Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9
title_fullStr	Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9
title_full_unstemmed	Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9
title_sort	inhibitory effects of andrographolide in pc-3 cell line and the induction of apoptosis via the involvement of caspases 3, 8 and 9
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Institut Penyelidikan Perubatan Molekul
publishDate	2023
url	http://eprints.usm.my/60182/1/24%20Pages%20from%20JANANY%20AP%20MANIMARAN.pdf
_version_	1794024101964152832

Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9

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