Antimycobacterial Potential Of Secondary Metabolites Of Actinobacteria From A Tropical Mangrove In Northern Peninsular Malaysia

Antimycobacterial Potential Of Secondary Metabolites Of Actinobacteria From A Tropical Mangrove In Northern Peninsular Malaysia

Actinobacteria play a crucial role in discovery of new antibiotics for diseases with known antimicrobial resistance, such as multidrug-resistant tuberculosis (TB) caused by Mycobacterium tuberculosis (MTB), which is becoming a major impediment to TB control worldwide. In this study, novel actinobact...

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Main Author: Adanan, Norshamiera
Format: Thesis
Language:English
Published: 2023
Subjects:
QH1-278.5 Natural history (General)
Online Access:http://eprints.usm.my/60194/1/24%20Pages%20from%20NORSHAMIERA%20BINTI%20ADANAN.pdf
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spelling my-usm-ep.601942024-03-15T00:58:54Z Antimycobacterial Potential Of Secondary Metabolites Of Actinobacteria From A Tropical Mangrove In Northern Peninsular Malaysia 2023-07 Adanan, Norshamiera QH1-278.5 Natural history (General) Actinobacteria play a crucial role in discovery of new antibiotics for diseases with known antimicrobial resistance, such as multidrug-resistant tuberculosis (TB) caused by Mycobacterium tuberculosis (MTB), which is becoming a major impediment to TB control worldwide. In this study, novel actinobacterial secondary metabolites with antimicrobial potential against MTB surrogate organisms were isolated from tropical mangroves in the northern region of Peninsular Malaysia. The objectives of this study were to 1) isolate and screen for antimycobacterial-producing actinobacteria in sediment and water samples from mangrove environments, 2) conduct crude extract evaluation using tetrazolium microplate assay (TEMA), checkerboard assay and time-kill assay to determine minimum inhibition and bactericidal concentration (MIC, MBC) and fractional inhibition concentration index (FICI), and 3) identify compounds through gas chromatography mass spectrometry (GCMS) analysis. A total of 54 mangrove actinobacterial isolates were isolated from three mangrove environments in Balik Pulau, Pulau Pinang, and Merbok, Kedah from water and sediment samples of four sites: crab holes, rhizosphere area, undisturbed area, and disturbed area. 2023-07 Thesis http://eprints.usm.my/60194/ http://eprints.usm.my/60194/1/24%20Pages%20from%20NORSHAMIERA%20BINTI%20ADANAN.pdf application/pdf en public masters Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kajihayat
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic QH1-278.5 Natural history (General)
spellingShingle QH1-278.5 Natural history (General)
Adanan, Norshamiera
Antimycobacterial Potential Of Secondary Metabolites Of Actinobacteria From A Tropical Mangrove In Northern Peninsular Malaysia
description Actinobacteria play a crucial role in discovery of new antibiotics for diseases with known antimicrobial resistance, such as multidrug-resistant tuberculosis (TB) caused by Mycobacterium tuberculosis (MTB), which is becoming a major impediment to TB control worldwide. In this study, novel actinobacterial secondary metabolites with antimicrobial potential against MTB surrogate organisms were isolated from tropical mangroves in the northern region of Peninsular Malaysia. The objectives of this study were to 1) isolate and screen for antimycobacterial-producing actinobacteria in sediment and water samples from mangrove environments, 2) conduct crude extract evaluation using tetrazolium microplate assay (TEMA), checkerboard assay and time-kill assay to determine minimum inhibition and bactericidal concentration (MIC, MBC) and fractional inhibition concentration index (FICI), and 3) identify compounds through gas chromatography mass spectrometry (GCMS) analysis. A total of 54 mangrove actinobacterial isolates were isolated from three mangrove environments in Balik Pulau, Pulau Pinang, and Merbok, Kedah from water and sediment samples of four sites: crab holes, rhizosphere area, undisturbed area, and disturbed area.
format Thesis
qualification_level Master's degree
author Adanan, Norshamiera
author_facet Adanan, Norshamiera
author_sort Adanan, Norshamiera
title Antimycobacterial Potential Of Secondary Metabolites Of Actinobacteria From A Tropical Mangrove In Northern Peninsular Malaysia
title_short Antimycobacterial Potential Of Secondary Metabolites Of Actinobacteria From A Tropical Mangrove In Northern Peninsular Malaysia
title_full Antimycobacterial Potential Of Secondary Metabolites Of Actinobacteria From A Tropical Mangrove In Northern Peninsular Malaysia
title_fullStr Antimycobacterial Potential Of Secondary Metabolites Of Actinobacteria From A Tropical Mangrove In Northern Peninsular Malaysia
title_full_unstemmed Antimycobacterial Potential Of Secondary Metabolites Of Actinobacteria From A Tropical Mangrove In Northern Peninsular Malaysia
title_sort antimycobacterial potential of secondary metabolites of actinobacteria from a tropical mangrove in northern peninsular malaysia
granting_institution Perpustakaan Hamzah Sendut
granting_department Pusat Pengajian Sains Kajihayat
publishDate 2023
url http://eprints.usm.my/60194/1/24%20Pages%20from%20NORSHAMIERA%20BINTI%20ADANAN.pdf
_version_ 1794024103679623168

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