Determination of corticosteroids in Malay traditional jamu using RP-HPLC-PDA method

Corticosteroid is a common adulterant found in herbal medicines which may bring adverse effectson health. In Malaysia, thereare reported cases highlighted the presence of corticosteroids in Jamu preparations. The aim of this study was to evaluate the sensitivity and effectiveness of a high-performan...

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Main Author:	Yet, Lew Chin
Format:	Thesis
Language:	English
Published:	2023
Subjects:	R Medicine RX Homeopathy
Online Access:	http://eprints.usm.my/60207/1/LEW%20CHIN%20YET-E.pdf
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id	my-usm-ep.60207
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spelling	my-usm-ep.602072024-03-19T06:50:06Z Determination of corticosteroids in Malay traditional jamu using RP-HPLC-PDA method 2023-09 Yet, Lew Chin R Medicine RX Homeopathy Corticosteroid is a common adulterant found in herbal medicines which may bring adverse effectson health. In Malaysia, thereare reported cases highlighted the presence of corticosteroids in Jamu preparations. The aim of this study was to evaluate the sensitivity and effectiveness of a high-performance liquid chromatography (HPLC) technique for the identification and quantification of prednisolone, hydrocortisone, and dexamethasone in conventional Jamupreparations produced in Malaysia. The study thoroughly examined samples from local Jamumarkets in Kelantan and online retail platforms like Lazada and Shopee to detect specific corticosteroids.The RP-HPLC-PDA system was utilized to detect and quantify the analytes, namelyprednisolone, hydrocortisone, and dexamethasone. The linearity of the calibration curve in detecting the selected steroids achieved an r2value of 0.999, 0.993, and 0.951, respectively. One of the analyzed samples wasfound to contain hydrocortisonewith concentration of 0.08mg –0.10 mg per capsule(%RSD = 0.19 -10.17%for repeatability test;12.14% for reproducibility test). The detection limit (LOD) for prednisolone and hydrocortisone is 1 ppm, while for 0.1 ppm for dexamethasone. For the quantification limit (LOQ), it is 3 ppm for prednisolone and hydrocortisone, and 0.4 ppm for dexamethasone. Theaccuracyof the results is indicated by the percent recovery values, which are 45%, 65%, and 75% respectively for prednisolone, hydrocortisone, anddexamethasone.The validated method is deemed to be suitable in determining corticosteroids in Jamuproducts. 2023-09 Thesis http://eprints.usm.my/60207/ http://eprints.usm.my/60207/1/LEW%20CHIN%20YET-E.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine RX Homeopathy
spellingShingle	R Medicine RX Homeopathy Yet, Lew Chin Determination of corticosteroids in Malay traditional jamu using RP-HPLC-PDA method
description	Corticosteroid is a common adulterant found in herbal medicines which may bring adverse effectson health. In Malaysia, thereare reported cases highlighted the presence of corticosteroids in Jamu preparations. The aim of this study was to evaluate the sensitivity and effectiveness of a high-performance liquid chromatography (HPLC) technique for the identification and quantification of prednisolone, hydrocortisone, and dexamethasone in conventional Jamupreparations produced in Malaysia. The study thoroughly examined samples from local Jamumarkets in Kelantan and online retail platforms like Lazada and Shopee to detect specific corticosteroids.The RP-HPLC-PDA system was utilized to detect and quantify the analytes, namelyprednisolone, hydrocortisone, and dexamethasone. The linearity of the calibration curve in detecting the selected steroids achieved an r2value of 0.999, 0.993, and 0.951, respectively. One of the analyzed samples wasfound to contain hydrocortisonewith concentration of 0.08mg –0.10 mg per capsule(%RSD = 0.19 -10.17%for repeatability test;12.14% for reproducibility test). The detection limit (LOD) for prednisolone and hydrocortisone is 1 ppm, while for 0.1 ppm for dexamethasone. For the quantification limit (LOQ), it is 3 ppm for prednisolone and hydrocortisone, and 0.4 ppm for dexamethasone. Theaccuracyof the results is indicated by the percent recovery values, which are 45%, 65%, and 75% respectively for prednisolone, hydrocortisone, anddexamethasone.The validated method is deemed to be suitable in determining corticosteroids in Jamuproducts.
format	Thesis
qualification_level	Master's degree
author	Yet, Lew Chin
author_facet	Yet, Lew Chin
author_sort	Yet, Lew Chin
title	Determination of corticosteroids in Malay traditional jamu using RP-HPLC-PDA method
title_short	Determination of corticosteroids in Malay traditional jamu using RP-HPLC-PDA method
title_full	Determination of corticosteroids in Malay traditional jamu using RP-HPLC-PDA method
title_fullStr	Determination of corticosteroids in Malay traditional jamu using RP-HPLC-PDA method
title_full_unstemmed	Determination of corticosteroids in Malay traditional jamu using RP-HPLC-PDA method
title_sort	determination of corticosteroids in malay traditional jamu using rp-hplc-pda method
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kesihatan
publishDate	2023
url	http://eprints.usm.my/60207/1/LEW%20CHIN%20YET-E.pdf
_version_	1794024106176282624

Determination of corticosteroids in Malay traditional jamu using RP-HPLC-PDA method

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