Combination effect of gallic acid and cisplatin on ros and GSH production of cervical cancer cells, hela

Cervical cancer is one of the leading causes of death among women worldwide. Unfortunately, most of the available cancer treatments reported to cause side effects. Therefore, patients turn to alternative treatments by utilizing natural products. Gallic acid (3,4,5‑trihydroxy benzoic acid; GA), a...

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Main Author:	Nazri, Batrisyia Mohd
Format:	Thesis
Language:	English
Published:	2023
Subjects:	R Medicine
Online Access:	http://eprints.usm.my/60246/1/Batrisyia-E.pdf
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id	my-usm-ep.60246
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spelling	my-usm-ep.602462024-04-15T08:27:20Z Combination effect of gallic acid and cisplatin on ros and GSH production of cervical cancer cells, hela 2023-08 Nazri, Batrisyia Mohd R Medicine RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Cervical cancer is one of the leading causes of death among women worldwide. Unfortunately, most of the available cancer treatments reported to cause side effects. Therefore, patients turn to alternative treatments by utilizing natural products. Gallic acid (3,4,5‑trihydroxy benzoic acid; GA), a plant‑derived natural phenolic compound, has been reported to prevent the development and progression of various types of cancers. Anticancer activities of GA were reported due to regulation of reactive oxygen species (ROS) and glutathione (GSH) at the cellular level. However, its mechanism of action is still unclear. Thus, this study aimed to evaluate the regulations of ROS and GSH on the combination of cisplatin and GA in HeLa cells at 24 and 48 hours treatment period at IC50 concentration. ROS levels, including H2O2 concentrations were measured in HeLa cells as these variations appear to influence cervical cancer's vulnerability to cisplatin treatment over both the 24-hour and 48-hour intervals. Moreover, GSH levels were measured in treated HeLa cells as it helps to provide information about the intricate balance between the body's attempts to counter oxidative stress and the response of cervical cancer cells to the damage caused by the treatment. The results revealed that the combination of cisplatin and GA led to distinct patterns of ROS production for both the 24-hour and 48-hour intervals. This highlights the substantial influence of ROS levels on the treatment efficacy. Additionally, H2O2 levels was elevated during the 48-hour period when cisplatin and GA were combined. This increase aligned with the initiation of apoptosis, highlighting the time-dependent nature of H2O2 levels production, as the combination treatment demonstrated increased concentration after 48 hours. Moreover, during the 48-hour treatment 2023-08 Thesis http://eprints.usm.my/60246/ http://eprints.usm.my/60246/1/Batrisyia-E.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine R Medicine
spellingShingle	R Medicine R Medicine Nazri, Batrisyia Mohd Combination effect of gallic acid and cisplatin on ros and GSH production of cervical cancer cells, hela
description	Cervical cancer is one of the leading causes of death among women worldwide. Unfortunately, most of the available cancer treatments reported to cause side effects. Therefore, patients turn to alternative treatments by utilizing natural products. Gallic acid (3,4,5‑trihydroxy benzoic acid; GA), a plant‑derived natural phenolic compound, has been reported to prevent the development and progression of various types of cancers. Anticancer activities of GA were reported due to regulation of reactive oxygen species (ROS) and glutathione (GSH) at the cellular level. However, its mechanism of action is still unclear. Thus, this study aimed to evaluate the regulations of ROS and GSH on the combination of cisplatin and GA in HeLa cells at 24 and 48 hours treatment period at IC50 concentration. ROS levels, including H2O2 concentrations were measured in HeLa cells as these variations appear to influence cervical cancer's vulnerability to cisplatin treatment over both the 24-hour and 48-hour intervals. Moreover, GSH levels were measured in treated HeLa cells as it helps to provide information about the intricate balance between the body's attempts to counter oxidative stress and the response of cervical cancer cells to the damage caused by the treatment. The results revealed that the combination of cisplatin and GA led to distinct patterns of ROS production for both the 24-hour and 48-hour intervals. This highlights the substantial influence of ROS levels on the treatment efficacy. Additionally, H2O2 levels was elevated during the 48-hour period when cisplatin and GA were combined. This increase aligned with the initiation of apoptosis, highlighting the time-dependent nature of H2O2 levels production, as the combination treatment demonstrated increased concentration after 48 hours. Moreover, during the 48-hour treatment
format	Thesis
qualification_level	Master's degree
author	Nazri, Batrisyia Mohd
author_facet	Nazri, Batrisyia Mohd
author_sort	Nazri, Batrisyia Mohd
title	Combination effect of gallic acid and cisplatin on ros and GSH production of cervical cancer cells, hela
title_short	Combination effect of gallic acid and cisplatin on ros and GSH production of cervical cancer cells, hela
title_full	Combination effect of gallic acid and cisplatin on ros and GSH production of cervical cancer cells, hela
title_fullStr	Combination effect of gallic acid and cisplatin on ros and GSH production of cervical cancer cells, hela
title_full_unstemmed	Combination effect of gallic acid and cisplatin on ros and GSH production of cervical cancer cells, hela
title_sort	combination effect of gallic acid and cisplatin on ros and gsh production of cervical cancer cells, hela
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2023
url	http://eprints.usm.my/60246/1/Batrisyia-E.pdf
_version_	1804888897433370624

Combination effect of gallic acid and cisplatin on ros and GSH production of cervical cancer cells, hela

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