Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver

Fasciola species, parasitic trematodes of mammals and humans, pose a significant public health risk and carry substantial socio-economic consequences. There have been few studies in Kelantan to characterise species, and this study focuses on their morphohistological characteristics and molecular...

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Main Author:	Omar, Abdullahi Abdirisak
Format:	Thesis
Language:	English
Published:	2023
Subjects:	R Medicine RC109-216 Infectious and parasitic diseases
Online Access:	http://eprints.usm.my/60284/1/Abdullahi%20Abdirisak%20Omar-E.pdf
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spelling	my-usm-ep.602842024-04-16T08:22:10Z Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver 2023-08 Omar, Abdullahi Abdirisak R Medicine RA440-440.87 Study and teaching. Research RC109-216 Infectious and parasitic diseases Fasciola species, parasitic trematodes of mammals and humans, pose a significant public health risk and carry substantial socio-economic consequences. There have been few studies in Kelantan to characterise species, and this study focuses on their morphohistological characteristics and molecular identification. Infected cattle livers were collected from several farms in Kelantan. Morphometric parameters for juvenile, medium, and adult flukes such as body width (BW), body length (BL), cone width (CW), and cone length (CL) were determined using a calibrated stereomicroscope. Fluke genomic DNA was extracted using a QIAGEN Mini kit, and polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) was performed on the 463 bp DNA fragment in the region of internal transcribed spacer 1 (ITS1) using Tas1 restriction enzyme. The PCR-RFLP product was separated on agarose gel and photographed, the livers were grossly investigated for Fasciola infection and morphological changes. Tissue samples were collected from histopathological examinations using H & E stain and the pathological macroscopic and microscopic changes of the liver and gall bladder were observed. The morphometric analysis of adult flukes revealed a BL of 4.260 ± 0.191cm, BW of 0.61 ± 0.180 cm, cephalic CW of 0.37 ± 0.057 cm, and CL of 0.28 ± 0.058 cm. The BL/BW and BW/CW ratios were 2.93-5.97 cm and 1.55-2.72 cm respectively. The PCR-RFLP produced 3 bands of 93 bp, 151 bp, and 220 bp, which indicated as F. gigantica. The parasite was found in the liver and gall bladder, and thickening of bile duct mucous was observed on macroscopic examination. Microscopic observation found infiltration of inflammatory cells, fibrosis, necrosis, and degeneration of hepatocytes. In the gall bladder, necrosis was found in the epithelial mucosal bile duct, infiltration of collagen fibers, inflammatory cells, hypertrophy, and hyperplasia of the bladder epithelium. The study found that the combination of both morphometric analysis and molecular methods can accurately confirm the presence of F. gigantica in the infected liver. Histopathological characterisation has demonstrated different morphological features of infected cattle liver. 2023-08 Thesis http://eprints.usm.my/60284/ http://eprints.usm.my/60284/1/Abdullahi%20Abdirisak%20Omar-E.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine R Medicine RC109-216 Infectious and parasitic diseases
spellingShingle	R Medicine R Medicine RC109-216 Infectious and parasitic diseases Omar, Abdullahi Abdirisak Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver
description	Fasciola species, parasitic trematodes of mammals and humans, pose a significant public health risk and carry substantial socio-economic consequences. There have been few studies in Kelantan to characterise species, and this study focuses on their morphohistological characteristics and molecular identification. Infected cattle livers were collected from several farms in Kelantan. Morphometric parameters for juvenile, medium, and adult flukes such as body width (BW), body length (BL), cone width (CW), and cone length (CL) were determined using a calibrated stereomicroscope. Fluke genomic DNA was extracted using a QIAGEN Mini kit, and polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) was performed on the 463 bp DNA fragment in the region of internal transcribed spacer 1 (ITS1) using Tas1 restriction enzyme. The PCR-RFLP product was separated on agarose gel and photographed, the livers were grossly investigated for Fasciola infection and morphological changes. Tissue samples were collected from histopathological examinations using H & E stain and the pathological macroscopic and microscopic changes of the liver and gall bladder were observed. The morphometric analysis of adult flukes revealed a BL of 4.260 ± 0.191cm, BW of 0.61 ± 0.180 cm, cephalic CW of 0.37 ± 0.057 cm, and CL of 0.28 ± 0.058 cm. The BL/BW and BW/CW ratios were 2.93-5.97 cm and 1.55-2.72 cm respectively. The PCR-RFLP produced 3 bands of 93 bp, 151 bp, and 220 bp, which indicated as F. gigantica. The parasite was found in the liver and gall bladder, and thickening of bile duct mucous was observed on macroscopic examination. Microscopic observation found infiltration of inflammatory cells, fibrosis, necrosis, and degeneration of hepatocytes. In the gall bladder, necrosis was found in the epithelial mucosal bile duct, infiltration of collagen fibers, inflammatory cells, hypertrophy, and hyperplasia of the bladder epithelium. The study found that the combination of both morphometric analysis and molecular methods can accurately confirm the presence of F. gigantica in the infected liver. Histopathological characterisation has demonstrated different morphological features of infected cattle liver.
format	Thesis
qualification_level	Master's degree
author	Omar, Abdullahi Abdirisak
author_facet	Omar, Abdullahi Abdirisak
author_sort	Omar, Abdullahi Abdirisak
title	Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver
title_short	Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver
title_full	Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver
title_fullStr	Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver
title_full_unstemmed	Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver
title_sort	morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2023
url	http://eprints.usm.my/60284/1/Abdullahi%20Abdirisak%20Omar-E.pdf
_version_	1804888905460219904

Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver

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