The determination of chimeric secretory IGA: tb epitopes in goat milk

Tuberculosis (TB) basically has two types: active and latent infections. The current vaccination against TB, which is Bacillus Calmette-Guerin (BCG), is unable to provide protection with high efficacy against active TB while showing no protection against latent infection. The administration of BC...

Full description

Saved in:

Bibliographic Details
Main Author:	Mustafa, Wahidah
Format:	Thesis
Language:	English
Published:	2023
Subjects:	R Medicine RC306-320.5 Tuberculosis RC31-1245 Internal medicine
Online Access:	http://eprints.usm.my/60288/1/Wahidah%20Mustafa-E.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.60288
record_format	uketd_dc
spelling	my-usm-ep.602882024-04-18T03:22:14Z The determination of chimeric secretory IGA: tb epitopes in goat milk 2023-08 Mustafa, Wahidah R Medicine RA440-440.87 Study and teaching. Research RC306-320.5 Tuberculosis RC31-1245 Internal medicine Tuberculosis (TB) basically has two types: active and latent infections. The current vaccination against TB, which is Bacillus Calmette-Guerin (BCG), is unable to provide protection with high efficacy against active TB while showing no protection against latent infection. The administration of BCG vaccination is systemic. Thus, the creation of mucosal tuberculosis vaccines with a focus on the protein constituent has become an area of research interest. The development of mucosal vaccines is difficult due to the nature of the mucosal system. In a previous study, our group developed the mucosal vaccine by combining the three different epitopes of TB, namely Ag85b, latency-associated protein (Acr), and resuscitation-promoting factor (Rpf) from Mycobacterium tuberculosis (Mtb), linked with secretory immunoglobulin A (sIgA). The construct has been cloned into the genome of the adeno-associated viral vector before being transduced into the mammary gland of the pregnant goat. Acr and Rpf are important components that are involved in the reactivation of dormant Mtb. Together with Ag85b, they have become important targets for the development of a new TB mucosal vaccine with the goal of preventing the reactivation of dormant tubercle bacilli associated with latent tuberculosis. In this study, the expression of the immunodominant epitope in the milk of the goat was evaluated using western blot with Rb pAb to Mycobacterium tuberculosis Ag85b. The result shows that a band at approximately 75 kDa was detected, indicating that the epitopes were successfully expressed in milk. The expression of chimeric proteins in different concentrations of goat milk is significant at p < 0.05. 2023-08 Thesis http://eprints.usm.my/60288/ http://eprints.usm.my/60288/1/Wahidah%20Mustafa-E.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine R Medicine RC306-320.5 Tuberculosis RC31-1245 Internal medicine
spellingShingle	R Medicine R Medicine RC306-320.5 Tuberculosis RC31-1245 Internal medicine Mustafa, Wahidah The determination of chimeric secretory IGA: tb epitopes in goat milk
description	Tuberculosis (TB) basically has two types: active and latent infections. The current vaccination against TB, which is Bacillus Calmette-Guerin (BCG), is unable to provide protection with high efficacy against active TB while showing no protection against latent infection. The administration of BCG vaccination is systemic. Thus, the creation of mucosal tuberculosis vaccines with a focus on the protein constituent has become an area of research interest. The development of mucosal vaccines is difficult due to the nature of the mucosal system. In a previous study, our group developed the mucosal vaccine by combining the three different epitopes of TB, namely Ag85b, latency-associated protein (Acr), and resuscitation-promoting factor (Rpf) from Mycobacterium tuberculosis (Mtb), linked with secretory immunoglobulin A (sIgA). The construct has been cloned into the genome of the adeno-associated viral vector before being transduced into the mammary gland of the pregnant goat. Acr and Rpf are important components that are involved in the reactivation of dormant Mtb. Together with Ag85b, they have become important targets for the development of a new TB mucosal vaccine with the goal of preventing the reactivation of dormant tubercle bacilli associated with latent tuberculosis. In this study, the expression of the immunodominant epitope in the milk of the goat was evaluated using western blot with Rb pAb to Mycobacterium tuberculosis Ag85b. The result shows that a band at approximately 75 kDa was detected, indicating that the epitopes were successfully expressed in milk. The expression of chimeric proteins in different concentrations of goat milk is significant at p < 0.05.
format	Thesis
qualification_level	Master's degree
author	Mustafa, Wahidah
author_facet	Mustafa, Wahidah
author_sort	Mustafa, Wahidah
title	The determination of chimeric secretory IGA: tb epitopes in goat milk
title_short	The determination of chimeric secretory IGA: tb epitopes in goat milk
title_full	The determination of chimeric secretory IGA: tb epitopes in goat milk
title_fullStr	The determination of chimeric secretory IGA: tb epitopes in goat milk
title_full_unstemmed	The determination of chimeric secretory IGA: tb epitopes in goat milk
title_sort	determination of chimeric secretory iga: tb epitopes in goat milk
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2023
url	http://eprints.usm.my/60288/1/Wahidah%20Mustafa-E.pdf
_version_	1804888906472095744

The determination of chimeric secretory IGA: tb epitopes in goat milk

Similar Items