Elucidating conserved sub-units from beta-coronavirus species for the creation of novel, cross-clade, and stable chimera sars-cov-2 spike proteins as future proof vaccine candidates

The COVID-19 pandemic caused by the SARS-CoV-2 outbreak triggered extensive scientific research. In this thesis, the spike (S) protein of the SARS-CoV-2 was studied in depth to gain useful insights to create a future proof vaccine. The structural information and structural stability of chimeric b...

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Main Author:	Susu, Wang
Format:	Thesis
Language:	English
Published:	2023
Subjects:	R Medicine RC109-216 Infectious and parasitic diseases
Online Access:	http://eprints.usm.my/60290/1/Wang%20Susu-E.pdf
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id	my-usm-ep.60290
record_format	uketd_dc
spelling	my-usm-ep.602902024-04-18T03:21:37Z Elucidating conserved sub-units from beta-coronavirus species for the creation of novel, cross-clade, and stable chimera sars-cov-2 spike proteins as future proof vaccine candidates 2023-08 Susu, Wang R Medicine RA440-440.87 Study and teaching. Research RC109-216 Infectious and parasitic diseases The COVID-19 pandemic caused by the SARS-CoV-2 outbreak triggered extensive scientific research. In this thesis, the spike (S) protein of the SARS-CoV-2 was studied in depth to gain useful insights to create a future proof vaccine. The structural information and structural stability of chimeric beta-coronavirus S proteins were investigated using bioinformatics techniques, structural predictions and molecular dynamics simulations. The study initially targeted the entire S protein of chimeric betacoronaviruses, but due to certain constraints, the study shifted the direction onto conserved regions of the S2 subunit only to generate chimeric sequences from different beta coronaviruses, in order to obtain vaccine candidates with broad immune coverage. For practicality, ten chimeric S2 sequences were carefully selected to study chimeric viral protein structures. This study used AlphaFold2 to predict 3D structures of the chimeric S2 protein sequences. Molecular dynamics simulations further elucidated their structural stabilities. The results lay the foundation for novel future proof vaccine design. 2023-08 Thesis http://eprints.usm.my/60290/ http://eprints.usm.my/60290/1/Wang%20Susu-E.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine R Medicine RC109-216 Infectious and parasitic diseases
spellingShingle	R Medicine R Medicine RC109-216 Infectious and parasitic diseases Susu, Wang Elucidating conserved sub-units from beta-coronavirus species for the creation of novel, cross-clade, and stable chimera sars-cov-2 spike proteins as future proof vaccine candidates
description	The COVID-19 pandemic caused by the SARS-CoV-2 outbreak triggered extensive scientific research. In this thesis, the spike (S) protein of the SARS-CoV-2 was studied in depth to gain useful insights to create a future proof vaccine. The structural information and structural stability of chimeric beta-coronavirus S proteins were investigated using bioinformatics techniques, structural predictions and molecular dynamics simulations. The study initially targeted the entire S protein of chimeric betacoronaviruses, but due to certain constraints, the study shifted the direction onto conserved regions of the S2 subunit only to generate chimeric sequences from different beta coronaviruses, in order to obtain vaccine candidates with broad immune coverage. For practicality, ten chimeric S2 sequences were carefully selected to study chimeric viral protein structures. This study used AlphaFold2 to predict 3D structures of the chimeric S2 protein sequences. Molecular dynamics simulations further elucidated their structural stabilities. The results lay the foundation for novel future proof vaccine design.
format	Thesis
qualification_level	Master's degree
author	Susu, Wang
author_facet	Susu, Wang
author_sort	Susu, Wang
title	Elucidating conserved sub-units from beta-coronavirus species for the creation of novel, cross-clade, and stable chimera sars-cov-2 spike proteins as future proof vaccine candidates
title_short	Elucidating conserved sub-units from beta-coronavirus species for the creation of novel, cross-clade, and stable chimera sars-cov-2 spike proteins as future proof vaccine candidates
title_full	Elucidating conserved sub-units from beta-coronavirus species for the creation of novel, cross-clade, and stable chimera sars-cov-2 spike proteins as future proof vaccine candidates
title_fullStr	Elucidating conserved sub-units from beta-coronavirus species for the creation of novel, cross-clade, and stable chimera sars-cov-2 spike proteins as future proof vaccine candidates
title_full_unstemmed	Elucidating conserved sub-units from beta-coronavirus species for the creation of novel, cross-clade, and stable chimera sars-cov-2 spike proteins as future proof vaccine candidates
title_sort	elucidating conserved sub-units from beta-coronavirus species for the creation of novel, cross-clade, and stable chimera sars-cov-2 spike proteins as future proof vaccine candidates
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2023
url	http://eprints.usm.my/60290/1/Wang%20Susu-E.pdf
_version_	1804888906718511104

Elucidating conserved sub-units from beta-coronavirus species for the creation of novel, cross-clade, and stable chimera sars-cov-2 spike proteins as future proof vaccine candidates

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