Fabrication Of 3d Double-network Cnc/alg/pegda Printed Constructs Seeded With C2c12 Myoblasts For Bioprinting Applications

3D bioprinting offers a promising alternative for articular cartilage repair and regeneration using a layer-by-layer predetermined movement from a computer aided (CAD) software. In this project, a bioink for bioprinting applications was formulated using cellulose nanocrystals (CNCs), alginate (Al...

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Main Author:	Asohan, Anusha Wei
Format:	Thesis
Language:	English
Published:	2023
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/60305/1/ANUSHA%20WEI%20AP%20ASOHAN%20-%20TESIS24.pdf
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id	my-usm-ep.60305
record_format	uketd_dc
spelling	my-usm-ep.603052024-03-29T08:53:47Z Fabrication Of 3d Double-network Cnc/alg/pegda Printed Constructs Seeded With C2c12 Myoblasts For Bioprinting Applications 2023-09 Asohan, Anusha Wei QH1-278.5 Natural history (General) 3D bioprinting offers a promising alternative for articular cartilage repair and regeneration using a layer-by-layer predetermined movement from a computer aided (CAD) software. In this project, a bioink for bioprinting applications was formulated using cellulose nanocrystals (CNCs), alginate (Alg), and poly(ethylene glycol) diacrylate (PEGDA). Firstly, different formulations of CNC/Alg/PEGDA were formulated using a double network crosslinking approach where the hydrogel was first ionically crosslinked with Ca2+ ions and subsequently photo-crosslinked under UV irradiation at 365nm post-extrusion. The rheological properties, swelling behaviour, and mechanical properties of the formulations were assessed to determine the optimal formulation for bioprinting. Then, the printability of the selected CNC/Alg/PEGDA formulation was evaluated using an extrusion based bioprinter. Next, the biocompatibility of the selected CNC/Alg/PEGDA formulation was determined using C2C12 myoblasts. The cells were seeded into the hydrogel, followed by the bioprinting process. The viability of cells was assessed using Live/Dead assay for a period of 14 to 16 days. The internal structure and porosity of the bioprinted construct with and without cells was analysed using scanning electron microscopy (SEM). Based on the preliminary results, Formulation 8 (F8) with the concentration of 4% CNC, 4% Alg and 40% PEGDA demonstrated shear-thinning behaviour with power-law inde 2023-09 Thesis http://eprints.usm.my/60305/ http://eprints.usm.my/60305/1/ANUSHA%20WEI%20AP%20ASOHAN%20-%20TESIS24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Asohan, Anusha Wei Fabrication Of 3d Double-network Cnc/alg/pegda Printed Constructs Seeded With C2c12 Myoblasts For Bioprinting Applications
description	3D bioprinting offers a promising alternative for articular cartilage repair and regeneration using a layer-by-layer predetermined movement from a computer aided (CAD) software. In this project, a bioink for bioprinting applications was formulated using cellulose nanocrystals (CNCs), alginate (Alg), and poly(ethylene glycol) diacrylate (PEGDA). Firstly, different formulations of CNC/Alg/PEGDA were formulated using a double network crosslinking approach where the hydrogel was first ionically crosslinked with Ca2+ ions and subsequently photo-crosslinked under UV irradiation at 365nm post-extrusion. The rheological properties, swelling behaviour, and mechanical properties of the formulations were assessed to determine the optimal formulation for bioprinting. Then, the printability of the selected CNC/Alg/PEGDA formulation was evaluated using an extrusion based bioprinter. Next, the biocompatibility of the selected CNC/Alg/PEGDA formulation was determined using C2C12 myoblasts. The cells were seeded into the hydrogel, followed by the bioprinting process. The viability of cells was assessed using Live/Dead assay for a period of 14 to 16 days. The internal structure and porosity of the bioprinted construct with and without cells was analysed using scanning electron microscopy (SEM). Based on the preliminary results, Formulation 8 (F8) with the concentration of 4% CNC, 4% Alg and 40% PEGDA demonstrated shear-thinning behaviour with power-law inde
format	Thesis
qualification_level	Master's degree
author	Asohan, Anusha Wei
author_facet	Asohan, Anusha Wei
author_sort	Asohan, Anusha Wei
title	Fabrication Of 3d Double-network Cnc/alg/pegda Printed Constructs Seeded With C2c12 Myoblasts For Bioprinting Applications
title_short	Fabrication Of 3d Double-network Cnc/alg/pegda Printed Constructs Seeded With C2c12 Myoblasts For Bioprinting Applications
title_full	Fabrication Of 3d Double-network Cnc/alg/pegda Printed Constructs Seeded With C2c12 Myoblasts For Bioprinting Applications
title_fullStr	Fabrication Of 3d Double-network Cnc/alg/pegda Printed Constructs Seeded With C2c12 Myoblasts For Bioprinting Applications
title_full_unstemmed	Fabrication Of 3d Double-network Cnc/alg/pegda Printed Constructs Seeded With C2c12 Myoblasts For Bioprinting Applications
title_sort	fabrication of 3d double-network cnc/alg/pegda printed constructs seeded with c2c12 myoblasts for bioprinting applications
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2023
url	http://eprints.usm.my/60305/1/ANUSHA%20WEI%20AP%20ASOHAN%20-%20TESIS24.pdf
_version_	1804888908949880832

Fabrication Of 3d Double-network Cnc/alg/pegda Printed Constructs Seeded With C2c12 Myoblasts For Bioprinting Applications

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