Immunoproteomic Analysis And Serodiagnosis Of Salmonella Enteretica Typhi And Invasive Non-Typhoidal Salmonella Infections Using Differentially Extracted Whole Cell Bacterial Protein

Typhoid fever is a potentially fatal infectious disease caused by Salmonella Typhi. The disease is endemic in developing countries, but alarming number of cases linked to domestic sources have been reported in industrialized countries. The clinical presentation of typhoid fever is highly variable...

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Main Author:	Abdul Lateef Khan, Khairunnisa
Format:	Thesis
Language:	English
Published:	2023
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/60327/1/24%20Pages%20from%20KHAIRUNNISA%20BINTI%20ABDUL%20LATEEF%20KHAN.pdf
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id	my-usm-ep.60327
record_format	uketd_dc
spelling	my-usm-ep.603272024-04-02T01:47:53Z Immunoproteomic Analysis And Serodiagnosis Of Salmonella Enteretica Typhi And Invasive Non-Typhoidal Salmonella Infections Using Differentially Extracted Whole Cell Bacterial Protein 2023-04 Abdul Lateef Khan, Khairunnisa R5-920 Medicine (General) Typhoid fever is a potentially fatal infectious disease caused by Salmonella Typhi. The disease is endemic in developing countries, but alarming number of cases linked to domestic sources have been reported in industrialized countries. The clinical presentation of typhoid fever is highly variable and often overlap with other acute undifferentiated febrile illnesses (AUFI). Hence, accurate identification of isolates is important for effective epidemiological intervention. The development of rapid, costeffective diagnostic test is also crucial for early life saving treatment. Therefore, comparative analysis was performed to characterize protein profiles of two differentially extracted Salmonella serovars by sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE). Separation of protein into three different extractions revealed discriminative protein profiles between the two strains which provided an effective adjunct that can be used as a tool for protein serotyping. A standardized protocol of the dot Enzyme Immunoassay (dot EIA) with differentially extracted antigens derived from S.Typhi and S.spp was established for definitive and differential diagnosis of typhoid fever. The performance of the test was encouraging with high sensitivity and specificity. Following the successful development of 'TYPHOIDYNE μSpot M-Ag Arrays, the antigenic components of the differentially extracted proteins were further characterized by western blot (WB) assay. 2023-04 Thesis http://eprints.usm.my/60327/ http://eprints.usm.my/60327/1/24%20Pages%20from%20KHAIRUNNISA%20BINTI%20ABDUL%20LATEEF%20KHAN.pdf application/pdf en public phd doctoral Perpustakaan Hamzah Sendut Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Abdul Lateef Khan, Khairunnisa Immunoproteomic Analysis And Serodiagnosis Of Salmonella Enteretica Typhi And Invasive Non-Typhoidal Salmonella Infections Using Differentially Extracted Whole Cell Bacterial Protein
description	Typhoid fever is a potentially fatal infectious disease caused by Salmonella Typhi. The disease is endemic in developing countries, but alarming number of cases linked to domestic sources have been reported in industrialized countries. The clinical presentation of typhoid fever is highly variable and often overlap with other acute undifferentiated febrile illnesses (AUFI). Hence, accurate identification of isolates is important for effective epidemiological intervention. The development of rapid, costeffective diagnostic test is also crucial for early life saving treatment. Therefore, comparative analysis was performed to characterize protein profiles of two differentially extracted Salmonella serovars by sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE). Separation of protein into three different extractions revealed discriminative protein profiles between the two strains which provided an effective adjunct that can be used as a tool for protein serotyping. A standardized protocol of the dot Enzyme Immunoassay (dot EIA) with differentially extracted antigens derived from S.Typhi and S.spp was established for definitive and differential diagnosis of typhoid fever. The performance of the test was encouraging with high sensitivity and specificity. Following the successful development of 'TYPHOIDYNE μSpot M-Ag Arrays, the antigenic components of the differentially extracted proteins were further characterized by western blot (WB) assay.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Abdul Lateef Khan, Khairunnisa
author_facet	Abdul Lateef Khan, Khairunnisa
author_sort	Abdul Lateef Khan, Khairunnisa
title	Immunoproteomic Analysis And Serodiagnosis Of Salmonella Enteretica Typhi And Invasive Non-Typhoidal Salmonella Infections Using Differentially Extracted Whole Cell Bacterial Protein
title_short	Immunoproteomic Analysis And Serodiagnosis Of Salmonella Enteretica Typhi And Invasive Non-Typhoidal Salmonella Infections Using Differentially Extracted Whole Cell Bacterial Protein
title_full	Immunoproteomic Analysis And Serodiagnosis Of Salmonella Enteretica Typhi And Invasive Non-Typhoidal Salmonella Infections Using Differentially Extracted Whole Cell Bacterial Protein
title_fullStr	Immunoproteomic Analysis And Serodiagnosis Of Salmonella Enteretica Typhi And Invasive Non-Typhoidal Salmonella Infections Using Differentially Extracted Whole Cell Bacterial Protein
title_full_unstemmed	Immunoproteomic Analysis And Serodiagnosis Of Salmonella Enteretica Typhi And Invasive Non-Typhoidal Salmonella Infections Using Differentially Extracted Whole Cell Bacterial Protein
title_sort	immunoproteomic analysis and serodiagnosis of salmonella enteretica typhi and invasive non-typhoidal salmonella infections using differentially extracted whole cell bacterial protein
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2023
url	http://eprints.usm.my/60327/1/24%20Pages%20from%20KHAIRUNNISA%20BINTI%20ABDUL%20LATEEF%20KHAN.pdf
_version_	1804888913449320448

Immunoproteomic Analysis And Serodiagnosis Of Salmonella Enteretica Typhi And Invasive Non-Typhoidal Salmonella Infections Using Differentially Extracted Whole Cell Bacterial Protein

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