Investigation Of In Vitro Permeation Of Mitragynine Through The Skin

Mitragynine is an alkaloid isolated from the leaves of the Mitragyna speciosa plant and has been traditionally consumed in the form of tea concoctions. Recent studies theorised mitragynine to be a potential candidate for pain relief and the treatment of opiate withdrawal symptoms as a replacement th...

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Main Author: Sim, Yee Shan
Format: Thesis
Language:English
Published: 2023
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Online Access:http://eprints.usm.my/60390/1/SIM%20YEE%20SHAN%20-%20TESIS%20cut.pdf
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spelling my-usm-ep.603902024-04-18T03:34:52Z Investigation Of In Vitro Permeation Of Mitragynine Through The Skin 2023-08 Sim, Yee Shan RS1-441 Pharmacy and materia medica Mitragynine is an alkaloid isolated from the leaves of the Mitragyna speciosa plant and has been traditionally consumed in the form of tea concoctions. Recent studies theorised mitragynine to be a potential candidate for pain relief and the treatment of opiate withdrawal symptoms as a replacement therapy. Due to mitragynine’s poor oral bioavailability, the delivery of mitragynine through the skin is proposed in this study. However, the investigation of in vitro permeation of mitragynine through the skin is deemed to be lacking. This study aims to investigate the potential of delivering mitragynine using various solvents through the skin, with emphasis to improve, optimise and validate methods to evaluate the permeation of mitragynine across the skin. Mitragynine was characterised using differential scanning calorimetry, attenuated total reflectance-Fourier transform infrared spectroscopy and x-ray diffraction. Mitragynine utilised in this experiment was found to be likely amorphous. A high-performance liquid chromatography analytical method with an ultraviolet detector was optimised and validated according to the International Council of Harmonisation guidelines. The analytical method was found to be precise, accurate and robust which successfully eluted a sharp mitragynine peak at ~4 min with an acceptable tailing factor of 1.32. The experimental method for the in vitro permeation studies using Franz diffusion cells was optimised and ascorbic acid was used as an antioxidant in the receptor compartment. In vitro permeation studies were conducted using dissolved mitragynine in simple solvent systems of various solvents. The solvents employed in this study were found to have the ability to solubilise and assist the permeation of mitragynine through the skin. 2023-08 Thesis http://eprints.usm.my/60390/ http://eprints.usm.my/60390/1/SIM%20YEE%20SHAN%20-%20TESIS%20cut.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Farmasi
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic RS1-441 Pharmacy and materia medica
spellingShingle RS1-441 Pharmacy and materia medica
Sim, Yee Shan
Investigation Of In Vitro Permeation Of Mitragynine Through The Skin
description Mitragynine is an alkaloid isolated from the leaves of the Mitragyna speciosa plant and has been traditionally consumed in the form of tea concoctions. Recent studies theorised mitragynine to be a potential candidate for pain relief and the treatment of opiate withdrawal symptoms as a replacement therapy. Due to mitragynine’s poor oral bioavailability, the delivery of mitragynine through the skin is proposed in this study. However, the investigation of in vitro permeation of mitragynine through the skin is deemed to be lacking. This study aims to investigate the potential of delivering mitragynine using various solvents through the skin, with emphasis to improve, optimise and validate methods to evaluate the permeation of mitragynine across the skin. Mitragynine was characterised using differential scanning calorimetry, attenuated total reflectance-Fourier transform infrared spectroscopy and x-ray diffraction. Mitragynine utilised in this experiment was found to be likely amorphous. A high-performance liquid chromatography analytical method with an ultraviolet detector was optimised and validated according to the International Council of Harmonisation guidelines. The analytical method was found to be precise, accurate and robust which successfully eluted a sharp mitragynine peak at ~4 min with an acceptable tailing factor of 1.32. The experimental method for the in vitro permeation studies using Franz diffusion cells was optimised and ascorbic acid was used as an antioxidant in the receptor compartment. In vitro permeation studies were conducted using dissolved mitragynine in simple solvent systems of various solvents. The solvents employed in this study were found to have the ability to solubilise and assist the permeation of mitragynine through the skin.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Sim, Yee Shan
author_facet Sim, Yee Shan
author_sort Sim, Yee Shan
title Investigation Of In Vitro Permeation Of Mitragynine Through The Skin
title_short Investigation Of In Vitro Permeation Of Mitragynine Through The Skin
title_full Investigation Of In Vitro Permeation Of Mitragynine Through The Skin
title_fullStr Investigation Of In Vitro Permeation Of Mitragynine Through The Skin
title_full_unstemmed Investigation Of In Vitro Permeation Of Mitragynine Through The Skin
title_sort investigation of in vitro permeation of mitragynine through the skin
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Farmasi
publishDate 2023
url http://eprints.usm.my/60390/1/SIM%20YEE%20SHAN%20-%20TESIS%20cut.pdf
_version_ 1804888928377896960