Identification Of Selected Ageing-Related Proteins And The Characterisation Of Ptc4 In Yeast

Ageing-related proteins play different roles in cellular processes such as regulating stress response, apoptosis, ubiquitin-proteasome system and signal transduction pathways amongst many others. Hundreds of ageing-related proteins have been revealed, but the roles of most of these ageing-related pr...

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Main Author:	Lee, Jee Whu
Format:	Thesis
Language:	English
Published:	2022
Subjects:	R735-854 Medical education Medical schools Research
Online Access:	http://eprints.usm.my/60425/1/LEE%20JEE%20WHU%20-%20TESIS%20cut.pdf
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spelling	my-usm-ep.604252024-04-25T03:07:21Z Identification Of Selected Ageing-Related Proteins And The Characterisation Of Ptc4 In Yeast 2022-12 Lee, Jee Whu R735-854 Medical education. Medical schools. Research Ageing-related proteins play different roles in cellular processes such as regulating stress response, apoptosis, ubiquitin-proteasome system and signal transduction pathways amongst many others. Hundreds of ageing-related proteins have been revealed, but the roles of most of these ageing-related proteins are still unknown. In this study, the standardised chronological life span (CLS) assay, stress assay and growth assay were established. The CLS-extending effects of the ageing-related proteins, Ptc4, Zwf1, Sme1 and Sod1 selected from previous chronological life span (CLS) screen were validated through standardised colony forming unit (CFU) assay and the deletions of the selected ageing-related genes resulted in decreased CLS in yeast. Ptc4 contributes to oxidative stress tolerance in standardised oxidative stress assay and extends CLS the most among the selected ageing-related proteins. Zwf1 and Sod1 that contribute to oxidative stress tolerance in young cells, did not show oxidative stress tolerance in cells after prolonged ageing, suggesting the loss of oxidative stress tolerance capability after prolonged ageing. Besides, Ptc4 and Zwf1 promoted cell proliferation during cell growth upon protein overexpression in standardised growth assays, suggesting their involvement in cell division or growth pathways. Furthermore, loss of Ptc4 could still promote cell proliferation while loss of Zwf1 did not affect rate of cell proliferation. As Ptc4 could promote cell proliferation, the effects of other yeast type 2C protein phosphatases (PP2Cs) on rate of cell proliferation were also investigated in standardised growth assays. 2022-12 Thesis http://eprints.usm.my/60425/ http://eprints.usm.my/60425/1/LEE%20JEE%20WHU%20-%20TESIS%20cut.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R735-854 Medical education Medical schools Research
spellingShingle	R735-854 Medical education Medical schools Research Lee, Jee Whu Identification Of Selected Ageing-Related Proteins And The Characterisation Of Ptc4 In Yeast
description	Ageing-related proteins play different roles in cellular processes such as regulating stress response, apoptosis, ubiquitin-proteasome system and signal transduction pathways amongst many others. Hundreds of ageing-related proteins have been revealed, but the roles of most of these ageing-related proteins are still unknown. In this study, the standardised chronological life span (CLS) assay, stress assay and growth assay were established. The CLS-extending effects of the ageing-related proteins, Ptc4, Zwf1, Sme1 and Sod1 selected from previous chronological life span (CLS) screen were validated through standardised colony forming unit (CFU) assay and the deletions of the selected ageing-related genes resulted in decreased CLS in yeast. Ptc4 contributes to oxidative stress tolerance in standardised oxidative stress assay and extends CLS the most among the selected ageing-related proteins. Zwf1 and Sod1 that contribute to oxidative stress tolerance in young cells, did not show oxidative stress tolerance in cells after prolonged ageing, suggesting the loss of oxidative stress tolerance capability after prolonged ageing. Besides, Ptc4 and Zwf1 promoted cell proliferation during cell growth upon protein overexpression in standardised growth assays, suggesting their involvement in cell division or growth pathways. Furthermore, loss of Ptc4 could still promote cell proliferation while loss of Zwf1 did not affect rate of cell proliferation. As Ptc4 could promote cell proliferation, the effects of other yeast type 2C protein phosphatases (PP2Cs) on rate of cell proliferation were also investigated in standardised growth assays.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Lee, Jee Whu
author_facet	Lee, Jee Whu
author_sort	Lee, Jee Whu
title	Identification Of Selected Ageing-Related Proteins And The Characterisation Of Ptc4 In Yeast
title_short	Identification Of Selected Ageing-Related Proteins And The Characterisation Of Ptc4 In Yeast
title_full	Identification Of Selected Ageing-Related Proteins And The Characterisation Of Ptc4 In Yeast
title_fullStr	Identification Of Selected Ageing-Related Proteins And The Characterisation Of Ptc4 In Yeast
title_full_unstemmed	Identification Of Selected Ageing-Related Proteins And The Characterisation Of Ptc4 In Yeast
title_sort	identification of selected ageing-related proteins and the characterisation of ptc4 in yeast
granting_institution	Universiti Sains Malaysia
granting_department	Institut Penyelidikan Perubatan Molekul
publishDate	2022
url	http://eprints.usm.my/60425/1/LEE%20JEE%20WHU%20-%20TESIS%20cut.pdf
_version_	1804888934152404992

Identification Of Selected Ageing-Related Proteins And The Characterisation Of Ptc4 In Yeast

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