Identification Of Selected Ageing-Related Proteins And The Characterisation Of Ptc4 In Yeast

Ageing-related proteins play different roles in cellular processes such as regulating stress response, apoptosis, ubiquitin-proteasome system and signal transduction pathways amongst many others. Hundreds of ageing-related proteins have been revealed, but the roles of most of these ageing-related pr...

全面介紹

Saved in:
書目詳細資料
主要作者: Lee, Jee Whu
格式: Thesis
語言:English
出版: 2022
主題:
在線閱讀:http://eprints.usm.my/60425/1/LEE%20JEE%20WHU%20-%20TESIS%20cut.pdf
標簽: 添加標簽
沒有標簽, 成為第一個標記此記錄!
id my-usm-ep.60425
record_format uketd_dc
spelling my-usm-ep.604252024-04-25T03:07:21Z Identification Of Selected Ageing-Related Proteins And The Characterisation Of Ptc4 In Yeast 2022-12 Lee, Jee Whu R735-854 Medical education. Medical schools. Research Ageing-related proteins play different roles in cellular processes such as regulating stress response, apoptosis, ubiquitin-proteasome system and signal transduction pathways amongst many others. Hundreds of ageing-related proteins have been revealed, but the roles of most of these ageing-related proteins are still unknown. In this study, the standardised chronological life span (CLS) assay, stress assay and growth assay were established. The CLS-extending effects of the ageing-related proteins, Ptc4, Zwf1, Sme1 and Sod1 selected from previous chronological life span (CLS) screen were validated through standardised colony forming unit (CFU) assay and the deletions of the selected ageing-related genes resulted in decreased CLS in yeast. Ptc4 contributes to oxidative stress tolerance in standardised oxidative stress assay and extends CLS the most among the selected ageing-related proteins. Zwf1 and Sod1 that contribute to oxidative stress tolerance in young cells, did not show oxidative stress tolerance in cells after prolonged ageing, suggesting the loss of oxidative stress tolerance capability after prolonged ageing. Besides, Ptc4 and Zwf1 promoted cell proliferation during cell growth upon protein overexpression in standardised growth assays, suggesting their involvement in cell division or growth pathways. Furthermore, loss of Ptc4 could still promote cell proliferation while loss of Zwf1 did not affect rate of cell proliferation. As Ptc4 could promote cell proliferation, the effects of other yeast type 2C protein phosphatases (PP2Cs) on rate of cell proliferation were also investigated in standardised growth assays. 2022-12 Thesis http://eprints.usm.my/60425/ http://eprints.usm.my/60425/1/LEE%20JEE%20WHU%20-%20TESIS%20cut.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic R735-854 Medical education
Medical schools
Research
spellingShingle R735-854 Medical education
Medical schools
Research
Lee, Jee Whu
Identification Of Selected Ageing-Related Proteins And The Characterisation Of Ptc4 In Yeast
description Ageing-related proteins play different roles in cellular processes such as regulating stress response, apoptosis, ubiquitin-proteasome system and signal transduction pathways amongst many others. Hundreds of ageing-related proteins have been revealed, but the roles of most of these ageing-related proteins are still unknown. In this study, the standardised chronological life span (CLS) assay, stress assay and growth assay were established. The CLS-extending effects of the ageing-related proteins, Ptc4, Zwf1, Sme1 and Sod1 selected from previous chronological life span (CLS) screen were validated through standardised colony forming unit (CFU) assay and the deletions of the selected ageing-related genes resulted in decreased CLS in yeast. Ptc4 contributes to oxidative stress tolerance in standardised oxidative stress assay and extends CLS the most among the selected ageing-related proteins. Zwf1 and Sod1 that contribute to oxidative stress tolerance in young cells, did not show oxidative stress tolerance in cells after prolonged ageing, suggesting the loss of oxidative stress tolerance capability after prolonged ageing. Besides, Ptc4 and Zwf1 promoted cell proliferation during cell growth upon protein overexpression in standardised growth assays, suggesting their involvement in cell division or growth pathways. Furthermore, loss of Ptc4 could still promote cell proliferation while loss of Zwf1 did not affect rate of cell proliferation. As Ptc4 could promote cell proliferation, the effects of other yeast type 2C protein phosphatases (PP2Cs) on rate of cell proliferation were also investigated in standardised growth assays.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Lee, Jee Whu
author_facet Lee, Jee Whu
author_sort Lee, Jee Whu
title Identification Of Selected Ageing-Related Proteins And The Characterisation Of Ptc4 In Yeast
title_short Identification Of Selected Ageing-Related Proteins And The Characterisation Of Ptc4 In Yeast
title_full Identification Of Selected Ageing-Related Proteins And The Characterisation Of Ptc4 In Yeast
title_fullStr Identification Of Selected Ageing-Related Proteins And The Characterisation Of Ptc4 In Yeast
title_full_unstemmed Identification Of Selected Ageing-Related Proteins And The Characterisation Of Ptc4 In Yeast
title_sort identification of selected ageing-related proteins and the characterisation of ptc4 in yeast
granting_institution Universiti Sains Malaysia
granting_department Institut Penyelidikan Perubatan Molekul
publishDate 2022
url http://eprints.usm.my/60425/1/LEE%20JEE%20WHU%20-%20TESIS%20cut.pdf
_version_ 1804888934152404992