Therapeutic Effects Of Extracellular Vesicles From Human Menstrual Blood-derived Mesenchymal Stem Cells In Acute Lung Injury Animal Model

Therapeutic Effects Of Extracellular Vesicles From Human Menstrual Blood-derived Mesenchymal Stem Cells In Acute Lung Injury Animal Model

Human menstrual blood-derived mesenchymal stem cells (MenSCs) have gained attention for their therapeutic potential in various conditions. However, their specific application and mechanism in treating Acute lung injury (ALI) is still being explored. The aim of this study was to assess the effectiven...

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Main Author: Lian, Jie
Format: Thesis
Language:English
Published: 2023
Subjects:
R5-920 Medicine (General)
Online Access:http://eprints.usm.my/60442/1/LIAN%20JIE%20-%20TESIS24.pdf
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spelling my-usm-ep.604422024-04-29T01:18:29Z Therapeutic Effects Of Extracellular Vesicles From Human Menstrual Blood-derived Mesenchymal Stem Cells In Acute Lung Injury Animal Model 2023-09 Lian, Jie R5-920 Medicine (General) Human menstrual blood-derived mesenchymal stem cells (MenSCs) have gained attention for their therapeutic potential in various conditions. However, their specific application and mechanism in treating Acute lung injury (ALI) is still being explored. The aim of this study was to assess the effectiveness and potential underlying mechanisms of MenSCs derived-extracellular vesicles (EVs) in ameliorating acute lung inflammation and injury in preclinical models. Initially, MenSCs or their EVs were obtained and administered intratracheally to a mouse model with ALI induced by lipopolysaccharide (LPS) in order to assess their protective effects against pulmonary inflammation and injury. This evaluation was performed using histological, molecular, bronchoalveolar lavage fluid (BALF), and mRNA-sequencing analyses, taking into account the optimal timing for intervention. Subsequently, a small RNA microarray technique was employed to identify potential microRNAs (miRNAs) that contribute to the improvement of pulmonary inflammation and injury mediated by MenSC-EVs in vivo. Additionally, the intrinsic capacity of these potential miRNAs or their target genes in regulating lung inflammation and injury was examined both in vivo and in vitro by depleting them using an adeno-associated virus (AAV) and lentivirus-mediated technique, respectively. Furthermore, conventional molecular and biochemical experiments were conducted to elucidate the underlying molecular mechanism 2023-09 Thesis http://eprints.usm.my/60442/ http://eprints.usm.my/60442/1/LIAN%20JIE%20-%20TESIS24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic R5-920 Medicine (General)
spellingShingle R5-920 Medicine (General)
Lian, Jie
Therapeutic Effects Of Extracellular Vesicles From Human Menstrual Blood-derived Mesenchymal Stem Cells In Acute Lung Injury Animal Model
description Human menstrual blood-derived mesenchymal stem cells (MenSCs) have gained attention for their therapeutic potential in various conditions. However, their specific application and mechanism in treating Acute lung injury (ALI) is still being explored. The aim of this study was to assess the effectiveness and potential underlying mechanisms of MenSCs derived-extracellular vesicles (EVs) in ameliorating acute lung inflammation and injury in preclinical models. Initially, MenSCs or their EVs were obtained and administered intratracheally to a mouse model with ALI induced by lipopolysaccharide (LPS) in order to assess their protective effects against pulmonary inflammation and injury. This evaluation was performed using histological, molecular, bronchoalveolar lavage fluid (BALF), and mRNA-sequencing analyses, taking into account the optimal timing for intervention. Subsequently, a small RNA microarray technique was employed to identify potential microRNAs (miRNAs) that contribute to the improvement of pulmonary inflammation and injury mediated by MenSC-EVs in vivo. Additionally, the intrinsic capacity of these potential miRNAs or their target genes in regulating lung inflammation and injury was examined both in vivo and in vitro by depleting them using an adeno-associated virus (AAV) and lentivirus-mediated technique, respectively. Furthermore, conventional molecular and biochemical experiments were conducted to elucidate the underlying molecular mechanism
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Lian, Jie
author_facet Lian, Jie
author_sort Lian, Jie
title Therapeutic Effects Of Extracellular Vesicles From Human Menstrual Blood-derived Mesenchymal Stem Cells In Acute Lung Injury Animal Model
title_short Therapeutic Effects Of Extracellular Vesicles From Human Menstrual Blood-derived Mesenchymal Stem Cells In Acute Lung Injury Animal Model
title_full Therapeutic Effects Of Extracellular Vesicles From Human Menstrual Blood-derived Mesenchymal Stem Cells In Acute Lung Injury Animal Model
title_fullStr Therapeutic Effects Of Extracellular Vesicles From Human Menstrual Blood-derived Mesenchymal Stem Cells In Acute Lung Injury Animal Model
title_full_unstemmed Therapeutic Effects Of Extracellular Vesicles From Human Menstrual Blood-derived Mesenchymal Stem Cells In Acute Lung Injury Animal Model
title_sort therapeutic effects of extracellular vesicles from human menstrual blood-derived mesenchymal stem cells in acute lung injury animal model
granting_institution Universiti Sains Malaysia
granting_department Institut Perubatan & Pergigian Termaju
publishDate 2023
url http://eprints.usm.my/60442/1/LIAN%20JIE%20-%20TESIS24.pdf
_version_ 1804888937638920192

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