Modulation Of Dopaminergic System By Mitragynine And The Underlying Mechanisms Instigating Impairment Of Hippocampal Synaptic Plasticity

Mitragynine is the primary indole alkaloid of kratom (M. speciosa) Korth and is thought to be part of kratom’s main psychoactive components. Besides contributing to the addiction, it is known to induce rewarding effects by acting as an agonist to opioid receptors and block the dopamine D2 receptor....

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Main Author:	Salim, Mohamad Azmeer Effendy Md
Format:	Thesis
Language:	English
Published:	2023
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/60582/1/MOHAMAD%20AZMEER%20EFFENDY%20BIN%20MD%20SALIM%20-%20TESIS24.pdf
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id	my-usm-ep.60582
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spelling	my-usm-ep.605822024-05-08T03:15:55Z Modulation Of Dopaminergic System By Mitragynine And The Underlying Mechanisms Instigating Impairment Of Hippocampal Synaptic Plasticity 2023-09 Salim, Mohamad Azmeer Effendy Md R5-920 Medicine (General) Mitragynine is the primary indole alkaloid of kratom (M. speciosa) Korth and is thought to be part of kratom’s main psychoactive components. Besides contributing to the addiction, it is known to induce rewarding effects by acting as an agonist to opioid receptors and block the dopamine D2 receptor. Moreover, mitragynine causes cognitive deficit and impairs the hippocampal synaptic plasticity, but the underlying mechanisms are still unknown. In the early part of the study, the modulation of dopaminergic system by mitragynine was investigated in rats treated with mitragynine (1 and 30 mg/kg) acutely (1-day treatment) and repeatedly (4-days treatment) through electroencephalography (EEG) recording. Level of dopamine release was quantified using electrochemical biosensor and ELISA kit analysis. The expression of dopamine transporter was measured using RT-qPCR. Results indicated that administration of mitragynine at both doses triggered changes in spectral frequencies demonstrating adaptations of dopaminergic system in freely moving rats. Repeated exposure of mitragynine (1 and 30 mg/kg) evoked increase of dopamine release after 4 days of administration, where at low dose (1 mg/kg) shows irregular increase of mitragynine released. High dose (30 mg/kg) shows a stable intensification of dopamine released, not after acute exposure in real time recording through an electrochemical biosensor. The adaptations of dopaminergic systems by mitragynine are coupled with increased expression of dopamine transporter in the prefrontal cortex. 2023-09 Thesis http://eprints.usm.my/60582/ http://eprints.usm.my/60582/1/MOHAMAD%20AZMEER%20EFFENDY%20BIN%20MD%20SALIM%20-%20TESIS24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Penyelidikan Dadah dan Ubat-ubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Salim, Mohamad Azmeer Effendy Md Modulation Of Dopaminergic System By Mitragynine And The Underlying Mechanisms Instigating Impairment Of Hippocampal Synaptic Plasticity
description	Mitragynine is the primary indole alkaloid of kratom (M. speciosa) Korth and is thought to be part of kratom’s main psychoactive components. Besides contributing to the addiction, it is known to induce rewarding effects by acting as an agonist to opioid receptors and block the dopamine D2 receptor. Moreover, mitragynine causes cognitive deficit and impairs the hippocampal synaptic plasticity, but the underlying mechanisms are still unknown. In the early part of the study, the modulation of dopaminergic system by mitragynine was investigated in rats treated with mitragynine (1 and 30 mg/kg) acutely (1-day treatment) and repeatedly (4-days treatment) through electroencephalography (EEG) recording. Level of dopamine release was quantified using electrochemical biosensor and ELISA kit analysis. The expression of dopamine transporter was measured using RT-qPCR. Results indicated that administration of mitragynine at both doses triggered changes in spectral frequencies demonstrating adaptations of dopaminergic system in freely moving rats. Repeated exposure of mitragynine (1 and 30 mg/kg) evoked increase of dopamine release after 4 days of administration, where at low dose (1 mg/kg) shows irregular increase of mitragynine released. High dose (30 mg/kg) shows a stable intensification of dopamine released, not after acute exposure in real time recording through an electrochemical biosensor. The adaptations of dopaminergic systems by mitragynine are coupled with increased expression of dopamine transporter in the prefrontal cortex.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Salim, Mohamad Azmeer Effendy Md
author_facet	Salim, Mohamad Azmeer Effendy Md
author_sort	Salim, Mohamad Azmeer Effendy Md
title	Modulation Of Dopaminergic System By Mitragynine And The Underlying Mechanisms Instigating Impairment Of Hippocampal Synaptic Plasticity
title_short	Modulation Of Dopaminergic System By Mitragynine And The Underlying Mechanisms Instigating Impairment Of Hippocampal Synaptic Plasticity
title_full	Modulation Of Dopaminergic System By Mitragynine And The Underlying Mechanisms Instigating Impairment Of Hippocampal Synaptic Plasticity
title_fullStr	Modulation Of Dopaminergic System By Mitragynine And The Underlying Mechanisms Instigating Impairment Of Hippocampal Synaptic Plasticity
title_full_unstemmed	Modulation Of Dopaminergic System By Mitragynine And The Underlying Mechanisms Instigating Impairment Of Hippocampal Synaptic Plasticity
title_sort	modulation of dopaminergic system by mitragynine and the underlying mechanisms instigating impairment of hippocampal synaptic plasticity
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Penyelidikan Dadah dan Ubat-ubatan
publishDate	2023
url	http://eprints.usm.my/60582/1/MOHAMAD%20AZMEER%20EFFENDY%20BIN%20MD%20SALIM%20-%20TESIS24.pdf
_version_	1804888968091664384

Modulation Of Dopaminergic System By Mitragynine And The Underlying Mechanisms Instigating Impairment Of Hippocampal Synaptic Plasticity

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