Investigation On Antiproliferative Mechanisms Of Alstonia Angustilobasilver Nanoparticles In Skin Squamous Cell Carcinoma

Investigation On Antiproliferative Mechanisms Of Alstonia Angustilobasilver Nanoparticles In Skin Squamous Cell Carcinoma

Traditional methods of nanoparticles synthesis frequently use toxics chemicals and substantial energy, which may have detrimental effects on the environment and human health. Recently, plant-based synthesis of nanoparticles (AgNPs) has emerged as a promising green synthesis method. Herein, we rep...

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Main Author: Ab Rahim, Nurhidayah
Format: Thesis
Language:English
Published: 2023
Subjects:
R5-920 Medicine (General)
Online Access:http://eprints.usm.my/60691/1/NURHIDAYAH%20BINTI%20AB%20RAHIM%20-%20TESIS24.pdf
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spelling my-usm-ep.606912024-06-04T09:11:24Z Investigation On Antiproliferative Mechanisms Of Alstonia Angustilobasilver Nanoparticles In Skin Squamous Cell Carcinoma 2023-08 Ab Rahim, Nurhidayah R5-920 Medicine (General) Traditional methods of nanoparticles synthesis frequently use toxics chemicals and substantial energy, which may have detrimental effects on the environment and human health. Recently, plant-based synthesis of nanoparticles (AgNPs) has emerged as a promising green synthesis method. Herein, we reported the green synthesis of AgNPs using the aqueous extract of A. angustiloba leaves and assessed its antiproliferative mechanisms. Initially, the physiochemical properties of A. angustiloba-AgNPs were characterised using UV–Vis spectrophotometry, FT-IR, FESEM, EDX, TEM, XRD and zeta sizer analyses. The cytotoxicity of A. angustiloba-AgNPs was examined by MTT assays against the A431 cancer cell line. The morphology of the treated cells was examined using fluorescence microscopy and the antiproliferative mechanisms of the nanoparticles in A431 cells were investigated by annexin-FITC/propidium iodide (PI) staining and DNA cell cycle analysis using flow cytometry. The intracellular ROS levels were measured using a commercially available kit. The expression of apoptosis and cell cycle-related proteins was determined by Western blotting. 2023-08 Thesis http://eprints.usm.my/60691/ http://eprints.usm.my/60691/1/NURHIDAYAH%20BINTI%20AB%20RAHIM%20-%20TESIS24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic R5-920 Medicine (General)
spellingShingle R5-920 Medicine (General)
Ab Rahim, Nurhidayah
Investigation On Antiproliferative Mechanisms Of Alstonia Angustilobasilver Nanoparticles In Skin Squamous Cell Carcinoma
description Traditional methods of nanoparticles synthesis frequently use toxics chemicals and substantial energy, which may have detrimental effects on the environment and human health. Recently, plant-based synthesis of nanoparticles (AgNPs) has emerged as a promising green synthesis method. Herein, we reported the green synthesis of AgNPs using the aqueous extract of A. angustiloba leaves and assessed its antiproliferative mechanisms. Initially, the physiochemical properties of A. angustiloba-AgNPs were characterised using UV–Vis spectrophotometry, FT-IR, FESEM, EDX, TEM, XRD and zeta sizer analyses. The cytotoxicity of A. angustiloba-AgNPs was examined by MTT assays against the A431 cancer cell line. The morphology of the treated cells was examined using fluorescence microscopy and the antiproliferative mechanisms of the nanoparticles in A431 cells were investigated by annexin-FITC/propidium iodide (PI) staining and DNA cell cycle analysis using flow cytometry. The intracellular ROS levels were measured using a commercially available kit. The expression of apoptosis and cell cycle-related proteins was determined by Western blotting.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Ab Rahim, Nurhidayah
author_facet Ab Rahim, Nurhidayah
author_sort Ab Rahim, Nurhidayah
title Investigation On Antiproliferative Mechanisms Of Alstonia Angustilobasilver Nanoparticles In Skin Squamous Cell Carcinoma
title_short Investigation On Antiproliferative Mechanisms Of Alstonia Angustilobasilver Nanoparticles In Skin Squamous Cell Carcinoma
title_full Investigation On Antiproliferative Mechanisms Of Alstonia Angustilobasilver Nanoparticles In Skin Squamous Cell Carcinoma
title_fullStr Investigation On Antiproliferative Mechanisms Of Alstonia Angustilobasilver Nanoparticles In Skin Squamous Cell Carcinoma
title_full_unstemmed Investigation On Antiproliferative Mechanisms Of Alstonia Angustilobasilver Nanoparticles In Skin Squamous Cell Carcinoma
title_sort investigation on antiproliferative mechanisms of alstonia angustilobasilver nanoparticles in skin squamous cell carcinoma
granting_institution Universiti Sains Malaysia
granting_department Institut Perubatan & Pergigian Termaju
publishDate 2023
url http://eprints.usm.my/60691/1/NURHIDAYAH%20BINTI%20AB%20RAHIM%20-%20TESIS24.pdf
_version_ 1804888986698645504

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