Study Of Goniothalamin And Bioactive Glass 45s5 (Gtn-bg) On Osteosarcoma (Saos-2) And Breast Adenocarcinoma (Mcf-7) Cells

Developing alternative cancer treatment is crucial due to the limitations of conventional chemotherapy, which can harm healthy cells while targeting cancer cells. Plant-derived natural products, with their diverse mechanisms and low toxicity, hold promise as cancer treatments, and their combination...

Full description

Saved in:

Bibliographic Details
Main Author:	Bakar, Siti Aishah Abu
Format:	Thesis
Language:	English
Published:	2023
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/60742/1/SITI%20AISHAH%20BINTI%20ABU%20BAKAR%20-%20TESIS24.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.60742
record_format	uketd_dc
spelling	my-usm-ep.607422024-06-24T01:47:12Z Study Of Goniothalamin And Bioactive Glass 45s5 (Gtn-bg) On Osteosarcoma (Saos-2) And Breast Adenocarcinoma (Mcf-7) Cells 2023-09 Bakar, Siti Aishah Abu R5-920 Medicine (General) Developing alternative cancer treatment is crucial due to the limitations of conventional chemotherapy, which can harm healthy cells while targeting cancer cells. Plant-derived natural products, with their diverse mechanisms and low toxicity, hold promise as cancer treatments, and their combination with bioactive materials may improve treatment effectiveness. In this study, the cytotoxic activity of a plant styryl-lactone, goniothalamin (GTN) was screened first in several human cancer cell lines and was found to possess a substantial range of cytotoxicity against osteosarcoma (Saos-2), breast adenocarcinoma (MCF-7), breast carcinoma (UACC-732), adenocarcinoma alveolar basal epithelial (A549) and colorectal adenocarcinoma (HT29) cells, but less toxicity towards human bone marrow-derived mesenchymal stem (HMSC) cells. GTN demonstrated selective toxicity towards cancer cells with a high SI value (>2) for each examined cancer cell line compared to doxorubicin (DOX). The potential enhancement of GTN's anticancer effects was explored by combining it with a sol-gel-derived bioactive glass 45S5 (BG 45S5) that possesses bioactive, biocompatible, and biodegradable properties. The combination of GTN-BG was found more potent than GTN in inhibiting the proliferation of Saos-2 and MCF-7 cells due to a better microenvironment provided with the release of ionic dissolution products such as Ca2+, Na+ and Mg2+ ions from the BG. For both GTN and GTN-BG treatments, several apoptotic features were detected when observed under a phase microscope, including cell shrinkage, rounded cells, and membrane blebbing. 2023-09 Thesis http://eprints.usm.my/60742/ http://eprints.usm.my/60742/1/SITI%20AISHAH%20BINTI%20ABU%20BAKAR%20-%20TESIS24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Bakar, Siti Aishah Abu Study Of Goniothalamin And Bioactive Glass 45s5 (Gtn-bg) On Osteosarcoma (Saos-2) And Breast Adenocarcinoma (Mcf-7) Cells
description	Developing alternative cancer treatment is crucial due to the limitations of conventional chemotherapy, which can harm healthy cells while targeting cancer cells. Plant-derived natural products, with their diverse mechanisms and low toxicity, hold promise as cancer treatments, and their combination with bioactive materials may improve treatment effectiveness. In this study, the cytotoxic activity of a plant styryl-lactone, goniothalamin (GTN) was screened first in several human cancer cell lines and was found to possess a substantial range of cytotoxicity against osteosarcoma (Saos-2), breast adenocarcinoma (MCF-7), breast carcinoma (UACC-732), adenocarcinoma alveolar basal epithelial (A549) and colorectal adenocarcinoma (HT29) cells, but less toxicity towards human bone marrow-derived mesenchymal stem (HMSC) cells. GTN demonstrated selective toxicity towards cancer cells with a high SI value (>2) for each examined cancer cell line compared to doxorubicin (DOX). The potential enhancement of GTN's anticancer effects was explored by combining it with a sol-gel-derived bioactive glass 45S5 (BG 45S5) that possesses bioactive, biocompatible, and biodegradable properties. The combination of GTN-BG was found more potent than GTN in inhibiting the proliferation of Saos-2 and MCF-7 cells due to a better microenvironment provided with the release of ionic dissolution products such as Ca2+, Na+ and Mg2+ ions from the BG. For both GTN and GTN-BG treatments, several apoptotic features were detected when observed under a phase microscope, including cell shrinkage, rounded cells, and membrane blebbing.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Bakar, Siti Aishah Abu
author_facet	Bakar, Siti Aishah Abu
author_sort	Bakar, Siti Aishah Abu
title	Study Of Goniothalamin And Bioactive Glass 45s5 (Gtn-bg) On Osteosarcoma (Saos-2) And Breast Adenocarcinoma (Mcf-7) Cells
title_short	Study Of Goniothalamin And Bioactive Glass 45s5 (Gtn-bg) On Osteosarcoma (Saos-2) And Breast Adenocarcinoma (Mcf-7) Cells
title_full	Study Of Goniothalamin And Bioactive Glass 45s5 (Gtn-bg) On Osteosarcoma (Saos-2) And Breast Adenocarcinoma (Mcf-7) Cells
title_fullStr	Study Of Goniothalamin And Bioactive Glass 45s5 (Gtn-bg) On Osteosarcoma (Saos-2) And Breast Adenocarcinoma (Mcf-7) Cells
title_full_unstemmed	Study Of Goniothalamin And Bioactive Glass 45s5 (Gtn-bg) On Osteosarcoma (Saos-2) And Breast Adenocarcinoma (Mcf-7) Cells
title_sort	study of goniothalamin and bioactive glass 45s5 (gtn-bg) on osteosarcoma (saos-2) and breast adenocarcinoma (mcf-7) cells
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2023
url	http://eprints.usm.my/60742/1/SITI%20AISHAH%20BINTI%20ABU%20BAKAR%20-%20TESIS24.pdf
_version_	1804888991955156992

Study Of Goniothalamin And Bioactive Glass 45s5 (Gtn-bg) On Osteosarcoma (Saos-2) And Breast Adenocarcinoma (Mcf-7) Cells

Similar Items