Phytochemical, Analgesic And Acute Toxicity Studies Of Uncaria Attenuata (Korth.) Leaf Extract

Uncaria species from the family of Rubiaceae is a type of climbing vine found across Southeast Asia, East Asia, and South America. The Uncaria leaves and hooks (claw-like) traditionally been used to treat pain, neurological disorders, hypertension, stroke, rheumatism and other ailments. The study ai...

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Main Author:	Ga, Tan Ai Fein A/p Ching
Format:	Thesis
Language:	English
Published:	2023
Subjects:	RS1-441 Pharmacy and materia medica
Online Access:	http://eprints.usm.my/60772/1/TAN%20AI%20FEIN%20AP%20CHING%20GA%20-%20TESIS24.pdf
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id	my-usm-ep.60772
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spelling	my-usm-ep.607722024-07-02T01:36:50Z Phytochemical, Analgesic And Acute Toxicity Studies Of Uncaria Attenuata (Korth.) Leaf Extract 2023-08 Ga, Tan Ai Fein A/p Ching RS1-441 Pharmacy and materia medica Uncaria species from the family of Rubiaceae is a type of climbing vine found across Southeast Asia, East Asia, and South America. The Uncaria leaves and hooks (claw-like) traditionally been used to treat pain, neurological disorders, hypertension, stroke, rheumatism and other ailments. The study aimed to extract, isolate, and characterize the alkaloid constituents from the leaves of Uncaria attenuata (Korth.). In the present study, female Sprague Dawley rats were evaluated for acute toxicity for 14 days. In order to evaluate the antinociceptive activity of U. attenuata extracts and isolated alkaloid, a rat hot plate model was carried out. Two alkaloids have been successfully isolated and purified using chromatographic techniques: column chromatography, thin-layer chromatography and preparative thin-layer chromatography. Structure elucidation was performed using 1D NMR (1H, 13C, DEPT 90, DEPT 135), 2D NMR (COSY, HMBC, HSQC and NOESY), UV-Vis, FTIR, GC-MS and LC-MS. For the acute toxicity study, the rats were fed orally and employed in series of 175, 550 and 2000 mg/kg following the OECD 425 guideline. The rats were evaluated for physical abnormalities, infection, differences in posture and movement, and mortality. The relative organ weight of the rats’ harvested organs was calculated. Subsequently, for the analgesic study, the methanolic extract (250, 500 mg/kg), alkaloid extract (50, 100 mg/kg), and villocarine A (5, 10, 20 mg/kg) were given orally to the rats, respectively. 2023-08 Thesis http://eprints.usm.my/60772/ http://eprints.usm.my/60772/1/TAN%20AI%20FEIN%20AP%20CHING%20GA%20-%20TESIS24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Farmasi
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RS1-441 Pharmacy and materia medica
spellingShingle	RS1-441 Pharmacy and materia medica Ga, Tan Ai Fein A/p Ching Phytochemical, Analgesic And Acute Toxicity Studies Of Uncaria Attenuata (Korth.) Leaf Extract
description	Uncaria species from the family of Rubiaceae is a type of climbing vine found across Southeast Asia, East Asia, and South America. The Uncaria leaves and hooks (claw-like) traditionally been used to treat pain, neurological disorders, hypertension, stroke, rheumatism and other ailments. The study aimed to extract, isolate, and characterize the alkaloid constituents from the leaves of Uncaria attenuata (Korth.). In the present study, female Sprague Dawley rats were evaluated for acute toxicity for 14 days. In order to evaluate the antinociceptive activity of U. attenuata extracts and isolated alkaloid, a rat hot plate model was carried out. Two alkaloids have been successfully isolated and purified using chromatographic techniques: column chromatography, thin-layer chromatography and preparative thin-layer chromatography. Structure elucidation was performed using 1D NMR (1H, 13C, DEPT 90, DEPT 135), 2D NMR (COSY, HMBC, HSQC and NOESY), UV-Vis, FTIR, GC-MS and LC-MS. For the acute toxicity study, the rats were fed orally and employed in series of 175, 550 and 2000 mg/kg following the OECD 425 guideline. The rats were evaluated for physical abnormalities, infection, differences in posture and movement, and mortality. The relative organ weight of the rats’ harvested organs was calculated. Subsequently, for the analgesic study, the methanolic extract (250, 500 mg/kg), alkaloid extract (50, 100 mg/kg), and villocarine A (5, 10, 20 mg/kg) were given orally to the rats, respectively.
format	Thesis
qualification_level	Master's degree
author	Ga, Tan Ai Fein A/p Ching
author_facet	Ga, Tan Ai Fein A/p Ching
author_sort	Ga, Tan Ai Fein A/p Ching
title	Phytochemical, Analgesic And Acute Toxicity Studies Of Uncaria Attenuata (Korth.) Leaf Extract
title_short	Phytochemical, Analgesic And Acute Toxicity Studies Of Uncaria Attenuata (Korth.) Leaf Extract
title_full	Phytochemical, Analgesic And Acute Toxicity Studies Of Uncaria Attenuata (Korth.) Leaf Extract
title_fullStr	Phytochemical, Analgesic And Acute Toxicity Studies Of Uncaria Attenuata (Korth.) Leaf Extract
title_full_unstemmed	Phytochemical, Analgesic And Acute Toxicity Studies Of Uncaria Attenuata (Korth.) Leaf Extract
title_sort	phytochemical, analgesic and acute toxicity studies of uncaria attenuata (korth.) leaf extract
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Farmasi
publishDate	2023
url	http://eprints.usm.my/60772/1/TAN%20AI%20FEIN%20AP%20CHING%20GA%20-%20TESIS24.pdf
_version_	1804888996928552960

Phytochemical, Analgesic And Acute Toxicity Studies Of Uncaria Attenuata (Korth.) Leaf Extract

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