Co2 Biofixation And Carbohydrate Biosynthesis By Locally Isolated Acidophilic Microalgae For Biobutanol Production Through Simultaneous Saccharification And Fermentation

Butanol is a common chemical that used as an additive for automotive fuel. Among the production methods, the biobutanol synthesised through acetone-butanol-ethanol (ABE) fermentation process. The ABE fermentation using microalgae biomass that contains high carbohydrate with less lignin, which is sui...

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Main Author:	Tan, Kean Meng
Format:	Thesis
Language:	English
Published:	2023
Subjects:	T1-995 Technology(General)
Online Access:	http://eprints.usm.my/60791/1/TAN%20KEAN%20MENG%20-%20TESIS24.pdf
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id	my-usm-ep.60791
record_format	uketd_dc
spelling	my-usm-ep.607912024-07-04T03:58:10Z Co2 Biofixation And Carbohydrate Biosynthesis By Locally Isolated Acidophilic Microalgae For Biobutanol Production Through Simultaneous Saccharification And Fermentation 2023-09 Tan, Kean Meng T1-995 Technology(General) Butanol is a common chemical that used as an additive for automotive fuel. Among the production methods, the biobutanol synthesised through acetone-butanol-ethanol (ABE) fermentation process. The ABE fermentation using microalgae biomass that contains high carbohydrate with less lignin, which is suitable to be biobutanol feedstock. The potential of microalgae for biological CO2 biosequestration, making them value-added compared to other bioresources. In addition, the promising single-step saccharification and fermentation (SSF) process during ABE fermentation, has opened up a novel ground for advancement in economic biobutanol production. Based on this study, a total of two native acidophilic microalgae were successfully isolated and were identified as Coccomyxa dispar and Scenedesmus parvus strains. The C. dispar and S. parvus exhibited highest in terms of biomass productivity, carbohydrate productivity, and CO2 biofixation when cultivated under the elevated condition. Apart from that, the carbohydrate-related genes and proteins were also been investigated in this study. Based on the transcriptomic analysis, the results showed that a significant upregulated of carbohydrate-related genes such as AGB, SS, ISA, AGPase, ME, G6PD, Accc, RuBC, and CA that involved in C. dispar, while PGM, AGB, SS, AGPase, ME, DGAT, RuBC, and CA involved in S. parvus 2023-09 Thesis http://eprints.usm.my/60791/ http://eprints.usm.my/60791/1/TAN%20KEAN%20MENG%20-%20TESIS24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Teknologi Industri
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	T1-995 Technology(General)
spellingShingle	T1-995 Technology(General) Tan, Kean Meng Co2 Biofixation And Carbohydrate Biosynthesis By Locally Isolated Acidophilic Microalgae For Biobutanol Production Through Simultaneous Saccharification And Fermentation
description	Butanol is a common chemical that used as an additive for automotive fuel. Among the production methods, the biobutanol synthesised through acetone-butanol-ethanol (ABE) fermentation process. The ABE fermentation using microalgae biomass that contains high carbohydrate with less lignin, which is suitable to be biobutanol feedstock. The potential of microalgae for biological CO2 biosequestration, making them value-added compared to other bioresources. In addition, the promising single-step saccharification and fermentation (SSF) process during ABE fermentation, has opened up a novel ground for advancement in economic biobutanol production. Based on this study, a total of two native acidophilic microalgae were successfully isolated and were identified as Coccomyxa dispar and Scenedesmus parvus strains. The C. dispar and S. parvus exhibited highest in terms of biomass productivity, carbohydrate productivity, and CO2 biofixation when cultivated under the elevated condition. Apart from that, the carbohydrate-related genes and proteins were also been investigated in this study. Based on the transcriptomic analysis, the results showed that a significant upregulated of carbohydrate-related genes such as AGB, SS, ISA, AGPase, ME, G6PD, Accc, RuBC, and CA that involved in C. dispar, while PGM, AGB, SS, AGPase, ME, DGAT, RuBC, and CA involved in S. parvus
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Tan, Kean Meng
author_facet	Tan, Kean Meng
author_sort	Tan, Kean Meng
title	Co2 Biofixation And Carbohydrate Biosynthesis By Locally Isolated Acidophilic Microalgae For Biobutanol Production Through Simultaneous Saccharification And Fermentation
title_short	Co2 Biofixation And Carbohydrate Biosynthesis By Locally Isolated Acidophilic Microalgae For Biobutanol Production Through Simultaneous Saccharification And Fermentation
title_full	Co2 Biofixation And Carbohydrate Biosynthesis By Locally Isolated Acidophilic Microalgae For Biobutanol Production Through Simultaneous Saccharification And Fermentation
title_fullStr	Co2 Biofixation And Carbohydrate Biosynthesis By Locally Isolated Acidophilic Microalgae For Biobutanol Production Through Simultaneous Saccharification And Fermentation
title_full_unstemmed	Co2 Biofixation And Carbohydrate Biosynthesis By Locally Isolated Acidophilic Microalgae For Biobutanol Production Through Simultaneous Saccharification And Fermentation
title_sort	co2 biofixation and carbohydrate biosynthesis by locally isolated acidophilic microalgae for biobutanol production through simultaneous saccharification and fermentation
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Teknologi Industri
publishDate	2023
url	http://eprints.usm.my/60791/1/TAN%20KEAN%20MENG%20-%20TESIS24.pdf
_version_	1804888997179162624

Co2 Biofixation And Carbohydrate Biosynthesis By Locally Isolated Acidophilic Microalgae For Biobutanol Production Through Simultaneous Saccharification And Fermentation

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